蛋白质PTM结果解读方式改变 pp. ERK 2 处理后 ERK 1 88% Phospho ERK 2 87% Phospho 处理前 ERK 1 7% Phospho ERK 2 8% Phospho pp. ERK 1 4 5 p. ERK 2 6 p. I * He. La 细胞对 EGF 处理的反应 ERK 2 7 8
Nanopro 1000 � 器概� 10
Nanopro 1000 � 器概� 11
Nanopro 1000 � 器概� 12
Nanopro 1000 � 器概� 13
Nanopro 1000 � 器概� 14
Nanopro 1000 � 器概� 15
Nanopro 1000 � 器概� 16
Nanopro 1000 � 器概� 17
Nanopro 1000�� 18
Nanopro 1000基��� 盒 ● 基��� 盒 XDR Charge Separation Master Kit For Peggy Sue, Peggy or Nano. Pro 1000 ��盒会根据需要�行更新,���前和 Protein. Simple�售人�确� 19
举例2:AKT 1磷酸化组合pattern
蛋白� isoform pattern与Biological response 32
33
responding patient (#104) with marrow complete response nonresponder (#111) whose disease progressed on treatment 42
蛋白� PTM profiling与疾病 43
蛋白� PTM profiling与疾病 44
nature protocols | VOL. 10 NO. 1 | 2015 | 149
nature protocols | VOL. 10 NO. 1 | 2015 | 149
patient-derived CML CD 34+CD 38+ committed progenitor cells (treated for 24 h with drug) 造血祖�胞 clinical CML CD 34+CD 38− primitive progenitor cells 原始祖�胞 Dasatinib,DAS,达沙替尼 Imatinib ,IM,伊�替尼 p-Crk. L peaks Chemiluminescence data for DAS and IM drug
Establishment of Namalwa cell lines stably expressing s. M-CSF and m. M-CSF 建立� 定表达膜型M-CSF和分泌型M-CSFBurkitt‘s淋巴瘤� 胞 系
LCM 切片中PTM isoform检测 LCM Samples WT K-RAS Anti-ERK 1/2 4. 5 5 5. 5 6 6. 5 Anti-ERK 1/2 200个�瘤� 胞 4. 5 mutant K-RAS 5 5. 5 6 6. 5 KRAS mutations result in elevated levels of the activated, duallyphosphorylated ERK 2 isoform 激光捕获显微切割样本(LCM) Nano proteomic analysis of extracellular receptor kinase-1/2 posttranslational activation in microdissected human hyperplastic colon lesions Proteomics,2012
��� 化 参考:Charge Assay Development v 3 55
p. I peak 确� 已有文献参考 CST网站数据�: https: //www. cellsignal. com/common/content. jsp? id=phositeplus 63