Lecture 1 Power Point Lecture Slides are prepared
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Lecture 1 Power. Point® Lecture Slides are prepared by Dr. Isaac Barjis, Biology Instructor MCAT Prep Exam Cell Structure and Function 1
Outline l Cellular Level of Organization l l Cell theory Cell size Prokaryotic Cells l Eukaryotic Cells l l l l Organelles Nucleus and Ribosome Endomembrane System Other Vesicles and Vacuoles Energy related organelles Cytoskeleton l Centrioles, Cilia, and Flagella 2
Cell Theory Cell was not discovered untill the development of Microscope l Detailed study of the cell began in the 1830 s l A unifying concept in biology l States that: l l l All organisms are composed of cells All cells come only from preexisting cells Cells are the smallest structural and functional unit of organisms Cells carry genetic information in the form of DNA 3
Sizes of Living Things 4
Cell Size Cells range in size from one millimeter down to one micrometer l Cells need a large surface area of plasma membrane to adequately exchange materials. l The surface‑area‑to‑volume ratio requires that cells be small l 5
Surface to Volume Ratio 6
Microscopy Today: Compound Light Microscope l Light passed through specimen l Focused by glass lenses l Max magnification about 1000 X l Resolves objects separated by 0. 2 mm, 500 X better than human eye l Resolution is limited by the wavelength of light (nanometer) 7
Compound Light Microscope l Diaphragm – controls amount of light – important for image contrast l Coarse Adjustment Knob – focuses the image l Fine Adjustment Knob – finely focuses the image 8
Microscopy Today: Transmission Electron Microscope l Abbreviated T. E. M. l Uses a beam of electrons to allow 100 fold higher magnification l Because it uses beam of electrons, its resolution is at the atomic level (picometer) l Tissue must be fixed and sectioned l Can living specimen be examined by T. E. M? 9
Transmission Electron Microscope 10
Microscopy Today: Immunofluorescence Light Microscope l Antibodies developed against a specific protein l l l Ultra-violet light (black light) passed through specimen l l l Fluorescent dye molecule attached to antibody molecules Specimen exposed to fluorescent antibodies Fluorescent dye glows in color where antigen is located Emitted light is focused by glass lenses onto human retina Allows mapping distribution of a specific protein in cell 11
Microscopy and Amoeba proteus 12
Cells Under the Microscope l phase-contrast light microscope - look at unstained living animal cells. l electron microscope - look at organelles e. g. ribosomes. l fluorescence microscope - look at a living cell expressing green fluorescent protein or to do confocal microscopy.
Autoradiography l Radioactive compounds decay or transform into other compounds or elements. l An autoradiograph is an image on an xray film or nuclear emulsion produced by the pattern of decay emissions (e. g. , beta particles or gamma rays) from a distribution of a radioactive substance l Autoradiography can also uses radioactive molecule to study biochemical activity, Protein synthesis 14
Cell Fractionation and Differential Centrifugation l Cell fractionation is the breaking apart of cellular components l Differential centrifugation: l Allows separation of cell parts l Separated out by size & density l Works like spin cycle of washer l The faster the machine spins, the smaller the parts that are settled out 15
Cell Fractionation and Differential Centrifugation 16
Eukaryotes Vs Prokaryotes Eukaryotic Cells Prokaryotic Cells The cells of “complex” organisms, including all plants, Protists, fungi and animals Contain a nucleus and membrane bound organelles “Simple” organisms, including bacteria and cyanobacteria (blue-green algae) Lack a nucleus and other membrane-encased organelles. Can specialize for certain functions, such as absorbing nutrients from food or transmitting nerve impulses; multicellular organs and organisms Cell Wall present in Plants and Fungi only Ribosome: 40 s, 60 S Usually exist as single, virtually identical cells Cell Wall Ribosome: 30 S, 50 S 17
The Structure of Bacteria l Occur in three basic shapes: l l Spherical coccus, Rod-shaped bacillus, Spiral spirillum (if rigid) or spirochete (if flexible). Cell Envelope includes: l l Plasma membrane - lipid bilayer with imbedded and peripheral protein Cell wall - maintains the shape of the cell 18
The Structure of Bacteria 19
The Structure of Bacteria 20
The Structure of Bacteria Cytoplasm & Appendages l Cytoplasm l Semifluid solution l l l Bounded by plasma membrane Contains water, inorganic and organic molecules, and enzymes. Nucleoid is a region that contains the single, circular DNA molecule. Plasmids are small accessory (extrachromosomal) rings of DNA Appendages l l l Flagella – Provide motility Fimbriae – small, bristle-like fibers that sprout from the cell surface Sex pili – rigid tubular structures used to pass DNA from cell to cell 21
Eukaryotic Cells l l Domain Eukarya includes: l Protists l Fungi l Plants l Animals Cells contain: l Membrane-bound nucleus that houses DNA l Specialized organelles l Plasma membrane l Much larger than prokaryotic cells l Some cells (e. g. , plant cells) have a cell wall 22
Hypothesized Origin of Eukaryotic Cells 23
Eukaryotic Cells: Organelles l Eukaryotic cells are compartmentalized l They contain small structures called organelles l l l Perform specific functions Isolates reactions from others Two classes of organelles: l Endomembrane system: l Organelles that communicate with one another l l l Via membrane channels Via small vesicles Energy related organelles l l Mitochondria & chloroplasts Basically independent & self-sufficient 24
Plasma Membrane 25
Animal Cell Anatomy 26
Plant Cell Anatomy 27
Cytosole l Cytosol, contains many long, fine filaments of protein that are responsible for cell shape and structure and thereby form the cell’s cytoskeleton 28
Nucleus Command center of cell, usually near center l Separated from cytoplasm by nuclear envelope l l Consists of double layer of membrane Nuclear pores permit exchange between nucleoplasm & cytoplasm Contains chromatin in semifluid nucleoplasm l l Chromatin contains DNA of genes, and proteins (Histones) Condenses to form chromosomes l l Chromosomes are formed during cell division Nucleolus is a dense structure in the nucleus l Synthesize ribosome RNA (r. RNA) 29
Anatomy of the Nucleus 30
Ribosomes l Are the site of protein synthesis in the cell l Composed of r. RNA and protein l l Consists of a large subunit and a small subunit l Subunits made in nucleolus May be located: l On the endoplasmic reticulum (thereby making it “rough”), or l Free in the cytoplasm 31
Nucleus, Ribosomes, & ER 32
Endomembrane System Series of intracellular membranes that compartmentalize the cell l Restrict enzymatic reactions to specific compartments within cell l Consists of: l l l Nuclear envelope Membranes of endoplasmic reticulum Golgi apparatus Vesicles l l Several types Transport materials between organelles of system 33
Endomembrane System: The Endoplasmic Reticulum A system of membrane channels and saccules (flattened vesicles) continuous with the outer membrane of the nuclear envelope l Rough ER l l l Studded with ribosomes on cytoplasmic side Protein anabolism l l Synthesizes proteins Modifies and processes proteins l l l Adds sugar to protein Results in glycoproteins Smooth ER l l No ribosomes Synthesis of lipids Site of various synthetic processes, detoxification, and storage Forms transport vesicles 34
Endoplasmic Reticulum 35
Endomembrane System: The Golgi Apparatus l Consists of flattened, curved saccules l Resembles stack of hollow pancakes l Modifies proteins and lipids l Receives vesicles from ER on cis (or inner face) l Modifies them and repackages them in vesicles l Release the vesicles from trans (or outer face) l Within cell l Export from cell (secretion, exocytosis) 36
Golgi Apparatus 37
Endomembrane System: Lysosomes l Membrane-bound vesicles (not in plants) l Produced by the Golgi apparatus l Contain powerful digestive enzymes and are highly acidic l Digestion of large molecules l Recycling of cellular debris and resources l Autolysis may occur in injured or dying cell to cause apoptosis (programmed cell death, like tadpole losing tail) 38
Lysosomes 39
Endomembrane System: Summary Proteins produced in rough ER and lipids from smooth ER are carried in vesicles to the Golgi apparatus. l The Golgi apparatus modifies these products and then sorts and packages them into vesicles that go to various cell destinations. l Secretory vesicles carry products to the membrane where exocytosis produces secretions. l Lysosomes fuse with incoming vesicles and digest macromolecules. l 40
Endomembrane System: A Visual Summary 41
Peroxisomes l Similar to lysosomes l l l Membrane-bounded vesicles Enclose enzymes that rid the cell of toxic peroxides Participate in the metabolism of fatty acids and many other metabolites 42
Peroxisomes 43
Vacuoles l Membranous sacs that are larger than vesicles l l l Store materials that occur in excess Others very specialized (contractile vacuole) Plants cells typically have a central vacuole l l Up to 90% volume of some cells Functions in: l l l Storage of water, nutrients, pigments, and waste products Development of turgor pressure Some functions performed by lysosomes in other eukaryotes 44
Vacuoles 45
Energy-Related Organelles: Chloroplast Structure l Bounded by double membrane l Inner membrane infolded l l Forms disc-like thylakoids, which are stacked to form grana l Suspended in semi-fluid stroma Chlorophyll l Green photosynthetic pigment l Chlorophyll capture solar energy 46
Energy-Related Organelles: Chloroplasts l Serve as the site of photosynthesis l Captures light energy to drive cellular machinery l Photosynthesis l Synthesizes carbohydrates from CO 2 & H 2 O l Makes own food using CO 2 as only carbon source l Inorganic molecules (Energy-poor compounds) are converted to organic molecules (energy-rich compounds) l Only plants, algae, and certain bacteria are capable of conducting photosynthesis 47
Chloroplast Structure 48
Energy-Related Organelles: Mitochondria l Smaller than chloroplast l Contain ribosomes and their own DNA l Surrounded by a double membrane l Inner membrane surrounds the matrix and is convoluted (folds) to form cristae. l Matrix – Inner semifluid containing respiratory enzymes l Break down carbohydrates l Involved in cellular respiration l Produce most of ATP utilized by the cell l Contain their own DNA and ribosome i. e. they are semiautonomous l Inherited from Oocyte 49
Mitochondrial Structure 50
Mitochondrial Origin Hypothesis 51
The Cytoskeleton l Maintains cell shape l Assists in movement of cell and organelles l Aids movement of materials in and out of cells l Three types of macromolecular fibers l l Microfilament l Intermediate Filaments l Microtubules Assemble and disassemble as needed 52
The Cytoskeleton: Actin Filaments Microfilament are rods of actin Extremely thin filaments like twisted pearl necklace l Support for microvilli in intestinal cells l Intracellular traffic control l l l For moving stuff around within cell Cytoplasmic streaming Function in pseudopods of amoeboid cells Important component in muscle contraction 53
The Cytoskeleton: Actin Filament Operation 54
The Cytoskeleton: Intermediate Filaments l Intermediate in size between actin filaments and microtubules l Rope-like assembly of fibrous polypeptides l Functions: l Support nuclear envelope l Cell-cell junctions, like those holding skin cells tightly together 55
The Cytoskeleton: Microtubules Hollow cylinders made of two globular proteins called a and b tubulin l Spontaneous pairing of a and b tubulin molecules form structures called dimers l Dimers then arrange themselves into tubular spirals of 13 dimers around l Assembly: l l Under control of Microtubule Organizing Center (MTOC) Most important MTOC is centrosome Function: l l l Provide framework for movement of organelle within cell Direct separation of chromosomes during cell division (e. g. Centrioles are composed of microtubules) Provide locomotion and movement (e. g. flagella and cilia) 56
The Cytoskeleton: Microtubule Operation 57
The Cytoskeleton 58
Microtubular Arrays: Centrioles l Short, hollow cylinders l One pair per animal cell l Located in centrosome of animal cells l Oriented at right angles to each other l Separate during mitosis to determine plane of division 59
Cytoskeleton: Centrioles 60
Microtubular Arrays: Cilia and Flagella Hair-like projections from cell surface that aid in cell movement l In eukaryotes, cilia are much shorter than flagella l l l Cilia move in coordinated waves like oars Flagella move like a propeller or cork screw 61
Structure of a Flagellum 62
Comparison of Prokaryotic and Eukaryotic Cells 63
Lecture 1 Power. Point® Lecture Slides are prepared by Dr. Isaac Barjis, Biology Instructor MCAT Exam Prep Membrane Structure and Function 64
Outline l Membrane Models l l Plasma Membrane Structure and Function l l l Phospholipids Proteins Plasma Membrane Permeability l l Fluid-Mosaic Diffusion Osmosis Transport Via Carrier Proteins Cell Surface Modifications 65
Structure and Function: The Phospholipid Bilayer l The plasma membrane is common to all cells l Separates: l l l Internal living cytoplasmic from External environment of cell Phospholipid bilayer: l External surface lined with hydrophilic polar heads l Cytoplasmic surface lined with hydrophilic polar heads l Nonpolar, hydrophobic, fatty-acid tails sandwiched in between 66
Unit Membrane 67
Membrane Models l Fluid-Mosaic Model l Three components: Basic membrane referred to as phospholipid bilayer l Protein molecules l Float around like icebergs on a sea l Membrane proteins may be peripheral or integral l l Peripheral proteins are found on the inner membrane surface Integral proteins are partially or wholly embedded (transmembrane) in the membrane Some have carbohydrate chains attached Cholesterol 68
The Fluid Mosaic Model 69
Transmembrane Proteins 70
Functions of Membrane Proteins l Channel Proteins: l l l Carrier Proteins: l l Provides unique chemical ID for cells Help body recognize foreign substances Receptor Proteins: l l l Combine with substance to be transported Assist passage of molecules through membrane Cell Recognition Proteins: l l Tubular Allow passage of molecules through membrane Binds with messenger molecule Causes cell to respond to message Enzymatic Proteins: l Carry out metabolic reactions directly 71
Membrane Protein Diversity 72
Science Focus: Cell Signaling 73
Types of Transport: Active vs. Passive l l l Plasma membrane is differentially (selectively) permeable l Allows some material to pass l Inhibits passage of other materials Passive Transport: l No ATP requirement l Molecules follow concentration gradient Active Transport l Requires carrier protein l Requires energy in form of ATP 74
Passage of Molecules Across the Membrane 75
Types of Membrane Transport: Overview 76
Types of Transport: Diffusion l A solution consists of: l l l A solvent (liquid), and A solute (dissolved solid) Diffusion l l Net movement of solute molecules down a concentration gradient Molecules move both ways along gradient Molecules move from high to low Equilibrium: l l When NET change stops Solute concentration uniform – no gradient 77
Gas Exchange in Lungs: Diffusion Across Lung 78
Types of Transport: Osmosis l Osmosis: l l l Special case of diffusion Focuses on solvent (water) movement rather than solute Diffusion of water across a differentially (selectively) permeable membrane l l l Solute concentration on one side high, but water concentration low Solute concentration on other side low, but water concentration high Water diffuses both ways across membrane but solute can’t Net movement of water is toward low water (high solute) concentration Osmotic pressure is the pressure that develops due to osmosis 79
Types of Transport: Carrier Proteins l Facilitated Transport Small molecules l Can’t get through membrane lipids l Combine with carrier proteins l Follow concentration gradient l l Active Transport Small molecules l Move against concentration gradient l Combining with carrier proteins l Requires energy l 80
Types of Transport: Carrier Proteins l Facilitated Transport Small molecules l Can’t get through membrane lipids l Combine with carrier proteins l Follow concentration gradient l l Active Transport Small molecules l Move against concentration gradient l Combining with carrier proteins l Requires energy l 81
Types of Transport: Membrane-Assisted Transport l Macromolecules transported into or out of the cell inside vesicles Exocytosis – Vesicles fuse with plasma membrane and secrete contents l Endocytosis – Cells engulf substances into pouch which becomes a vesicle l Phagocytosis – Large, solid material into vesicle l Pinocytosis – Liquid or small, solid particles go into vesicle l Receptor-Mediated – Specific form of pinocytosis using a coated pit l 82
Cell Surface Modifications: Junctions l Cell l Surfaces in Animals Junctions Between Cells l Adhesion Junctions l l Tight Junctions l l Intercellular filaments between cells Form impermeable barriers Gap Junctions l Plasma membrane channels are joined (allows communication) 83
Cell Surface Modifications l l Extracellular Matrix l External meshwork of polysaccharides and proteins l Found in close association with the cell that produced them Plant Cell Walls l Plants have freely permeable cell wall, with cellulose as the main component l Plasmodesmata penetrate cell wall l Each contains a strand of cytoplasm l Allow passage of material between cells 84
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