31 Dr James Watson codiscoverer of the DNA

День Генома (31 мая) Dr. James Watson, co-discoverer of the DNA helix and father of the Human Genome Project, became the first human to receive the data encompassing his personal genome sequence at Baylor College of Medicine in Houston in May 31, 2007. James Watson (left) receives a digital copy of his genome sequence from Jonathan Rothberg in May 2007.

Эволюция геномных проектов • • • Human Genome Project Jim 1000 Genomes Project 100, 000 Genomes Project Precision Medicine Initiative (PMI) (1 миллион геномов) Mission statement (PMI): To enable a new era of medicine through research, technology, and policies that empower patients, researchers, and providers to work together toward development of individualized care. 1990… 2003 2005… 2007 2008… 2015 2012… 2017 2016… 2019

“Нацпроекты” • • • Saudi human genome project (SGP) ~ 33 млн. геномов Qatar genome project (QGP) ~ 0, 3 млн. геномов Iceland’s Genome Project ~ 0, 3 млн. геномов Korea's Personal Genome Project ~ 51 млн. геномов China Precision Medicine Initiative 100 млн. геномов ($9, 2 млрд. ; 15 лет)

Секвенаторы первой пятилетки 2005 2006 2007 2008 2009 2010 GS 20 GS FLX GA 1 G Titanium GS Junior GA II……x………e Hi. Seq 2000 SOLi. D 2. 0 SOLi. D 3 2011 FLX+ Hi. Seq 1000 Mi. Seq SOLi. D 4 5500 xl 5500 PGM Pac. Bio RS Max. Seq

Лидеры 2012 года 1 Tbp 100 Gbp 10 Gbp Ion Proton Mi. Seq 1 Gbp PGM

Секвенаторы 2017 года 1 Tbp Prometh. ION Nova. Seq 6000 100 Gbp BGISEQ-500 Grid. ION X 5 10 Gbp Min. ION Ion S 5 Mini. Sequel BGISEQ-50

Лидеры WGS $850, 000 $985, 000 http: //enseqlopedia. com/2017/01/almost-everything-wanted-know-illumina-novaseqand-stuff-didnt/

Ценовая политика http: //enseqlopedia. com/2017/01/almost-everything-wanted-know-illumina-novaseqand-stuff-didnt/

Полупроводниковый секвенатор (Ion Torrent / Life Technologies / Thermo Fisher Scientific) Ion S 5

Последняя модель https: //s 3. amazonaws. com/files. pacb. com/pdf/2015_11_Marty+Badgett+Pac. Bio+-+RSII+%26+Sequel. pdf

География SMRT-секвенирования http: //www. pacb. com/wp-content/uploads/2015/09/Pac. Bio_Sequencing_Providers. pdf

Presentation of Oxford Nanopore technology at @IBV_CSIC Published on Mar 9, 2017 https: //www. slideshare. net/Venmica. Estructuraly. F/oxford-nanopore-minion

http: //omics. blogspot. ru/2016/03/oxfords-no-thanks-ive-already-got-one. html

Точность нанопорового секвенирования (Min. ION) https: //www. slideshare. net/Venmica. Estructuraly. F/oxford-nanopore-minion

Особенности национального секвенирования Next. Seq CN 500 BGISEQ-50 BGISEQ-100 BGISEQ-500 HYK-PSTAR-IIA (Direct Genomics) BGISEQ-1000 Bioelectron Seq 4000 DA 8600 Geno. Care

Лучший китайский секвенатор (бета-тестирование BGISEQ-500) A reference human genome dataset of the BGISEQ-500 sequencer. Huang J. , Liang X. , Xuan Y. et al. Gigascience. 2017 Apr 1. doi: 10. 1093/gigascience/gix 024. [Epub ahead of print]

Самодельный геномный секвенатор https: //www. youtube. com/watch? v=eojg 02 AUAxw