CHAPTER 7 A TOUR OF THE CELL q

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CHAPTER 7 A TOUR OF THE CELL

CHAPTER 7 A TOUR OF THE CELL

q. Cytology: science/study of cells q Light microscopy resolving power~ measure of clarity q

q. Cytology: science/study of cells q Light microscopy resolving power~ measure of clarity q Electron microscopy TEM ~ electron beam to study cell ultrastructure (internal anatomy) SEM ~ electron beam to study cell surfaces q Cell fractionation ~ cell separation; organelle study q Ultracentrifuge ~ cell fractionation; 130, 000 rpm

A cell is a living unit greater than the sum of its parts •

A cell is a living unit greater than the sum of its parts • While the cell has many structures that have specific functions, they must work together. • Cell Theory – Cells are the basic unit of structure and function – All cell arise from other cells – All living things are made of one or more cells

Cell Types: Prokaryotic Domains: Bacteria and Archaea q. Nucleoid: DNA concentration q. No membrane

Cell Types: Prokaryotic Domains: Bacteria and Archaea q. Nucleoid: DNA concentration q. No membrane bound organelles q. Ribosomes: protein synthesis q. Plasma membrane: (all cells); semi-permeable q. Cytoplasm/cytosol(all cells)

Cell types: Eukaryotic Domains: Protist, fungi, Plants, and Animals q. Nucleus: membrane enclosed organelle

Cell types: Eukaryotic Domains: Protist, fungi, Plants, and Animals q. Nucleus: membrane enclosed organelle containing chromosomes q. Membrane bound organelles of specialized form and function q Generally larger than prokaryotic cells

Cell Size q As cell size increases, the surface area to volume ratio decreases

Cell Size q As cell size increases, the surface area to volume ratio decreases (as well as the % Diffusion) q Rates of chemical exchange may then be inadequate for cell size q Cell size, therefore, remains small

Nucleus q. Genetic material… § chromatin § Chromosomes § Nucleolus: r. RNA; ribosome synthesis

Nucleus q. Genetic material… § chromatin § Chromosomes § Nucleolus: r. RNA; ribosome synthesis q. Nuclear Envelope: double membrane with pores m. RNA~ protein synthesis

Ribosomes q. Protein manufacture q Types: a) free cytosol; protein function in cell b)

Ribosomes q. Protein manufacture q Types: a) free cytosol; protein function in cell b) bound: on ER; proteins function in membranes, organelles and export

The Endomembrane System q Endoplasmic reticulum(ER) q Continuous with nuclear envelope q Smooth ER

The Endomembrane System q Endoplasmic reticulum(ER) q Continuous with nuclear envelope q Smooth ER § § no ribosomes Synthesis of lipids Metabolism of carbohydrates Detoxification of drugs &poisons q Rough ER § With ribosomes § Synthesis of secretory proteins (glycoproteins) § Membrane production

The Golgi apparatus q ER products are modified, stored, and then shipped to either:

The Golgi apparatus q ER products are modified, stored, and then shipped to either: lysosomes, central vacuole, plasma membrane q Cisternae: flattened membranous sacs q Trans face(shipping) & cis face (receiving) q Transport vesicles

Lysosomes q Sac of hydrolytic enzymes; digestion of macromolecules q Phagocytosis q Autophagy: recycle

Lysosomes q Sac of hydrolytic enzymes; digestion of macromolecules q Phagocytosis q Autophagy: recycle cell’s own organic material q Tay-Sachs disease~ lipid digestions disorder

Vacuoles q. Membrane-bound sacs(larger than vesicles) q. Food (phagocytosis) q. Contractile (pump excess water)

Vacuoles q. Membrane-bound sacs(larger than vesicles) q. Food (phagocytosis) q. Contractile (pump excess water) q. Central (storage in plants as well as lysosomal functions) § Tonoplast membrane

Other Membranous Organelles 1. Mitochondria and chloroplasts are the main energy transformers of cells

Other Membranous Organelles 1. Mitochondria and chloroplasts are the main energy transformers of cells • • Both organelles have small quantities of DNA that direct the synthesis of the polypeptides produced by their internal ribosomes. Mitochondria and chloroplasts grow and reproduce as semiautonomous organelles. 2. Peroxisomes generate and degrade H 2 O 2 in performing various metabolic functions • What enzyme breaks down H 2 O 2?

Mitochondria q Site of cellular respiration q have a smooth outer membrane and a

Mitochondria q Site of cellular respiration q have a smooth outer membrane and a highly folded inner membrane, the cristae q inner membrane encloses the mitochondrial matrix, a fluid-filled space with DNA, ribosomes, and enzymes.

chloroplasts qfound in plants, and eukaryotic algae (protista) qsite of photosynthesis. q. Inside the

chloroplasts qfound in plants, and eukaryotic algae (protista) qsite of photosynthesis. q. Inside the innermost membrane is a fluidfilled space, the stroma, in which float membranous sacs, the thylakoids.

Peroxisomes • generate and degrade H 2 O 2 in performing various metabolic functions

Peroxisomes • generate and degrade H 2 O 2 in performing various metabolic functions • bounded by a single membrane. • They form not from the endomembrane system, but by incorporation of proteins and lipids from the cytosol.

The Cytoskeleton • Providing structural support to the cell, the cytoskeleton also functions in

The Cytoskeleton • Providing structural support to the cell, the cytoskeleton also functions in cell motility and regulation

There are three main types of fibers in the cytoskeleton: microtubules, microfilaments, and intermediate

There are three main types of fibers in the cytoskeleton: microtubules, microfilaments, and intermediate filaments.

Microtubules • the thickest fibers, are hollow rods about 25 microns in diameter. •

Microtubules • the thickest fibers, are hollow rods about 25 microns in diameter. • They move chromosomes during cell division. • Another function is as tracks that guide motor proteins carrying organelles to their destination.

cilia and flagella. • Microtubules are the central structural support • Cilia usually occur

cilia and flagella. • Microtubules are the central structural support • Cilia usually occur in large numbers on the cell surface. • There are usually just one or a few flagella per cell

cilia and flagella. • A flagellum has an undulatory movement

cilia and flagella. • A flagellum has an undulatory movement

cilia and flagella. • Cilia move more like oars with alternating power and recovery

cilia and flagella. • Cilia move more like oars with alternating power and recovery strokes.

cilia and flagella • have the same ultrastructure.

cilia and flagella • have the same ultrastructure.

Microfilaments • the thinnest class of the cytoskeletal fibers, are solid rods of the

Microfilaments • the thinnest class of the cytoskeletal fibers, are solid rods of the globular protein actin. designed to resist tension • form a three-dimensional network just inside the plasma membrane.

Microfilaments • In muscle cells, thousands of actin filaments are arranged parallel to one

Microfilaments • In muscle cells, thousands of actin filaments are arranged parallel to one another. • Thicker filaments, composed of a motor protein, myosin, interdigitate with the thinner actin fibers

Microfilaments • In other cells, these actin-myosin aggregates are less organized but still cause

Microfilaments • In other cells, these actin-myosin aggregates are less organized but still cause localized contraction • Pseudopodia, cellular extensions, extend and contract through the reversible assembly and contraction of actin subunits into microfilaments.

Microfilaments • In plant cells (and others), actin-myosin interactions and sol-gel transformations drive cytoplasmic

Microfilaments • In plant cells (and others), actin-myosin interactions and sol-gel transformations drive cytoplasmic streaming.

Intermediate filaments, • more permanent fixtures of the cytoskeleton than are the other two

Intermediate filaments, • more permanent fixtures of the cytoskeleton than are the other two classes • reinforce cell shape • and fix organelle location.

Cell Surfaces and Junctions 1. Plant cells are encased by cell walls 2. The

Cell Surfaces and Junctions 1. Plant cells are encased by cell walls 2. The extracellular matrix (ECM) of animal cells functions in support, adhesion, movement, and regulation 3. Intercellular junctions help integrate cells into higher levels of structure and function 4. The cell is a living unit greater than the sum of its parts

Plant cells are encased by cell walls • The cell wall, found in prokaryotes,

Plant cells are encased by cell walls • The cell wall, found in prokaryotes, fungi, and some protists, has multiple functions. • In plants, the cell wall protects the cell, maintains its shape, and prevents excessive uptake of water. • It also supports the plant against the force of gravity.

A mature cell wall consists of a primary cell wall, a middle lamella with

A mature cell wall consists of a primary cell wall, a middle lamella with sticky polysaccharides that holds cell together, and layers of secondary cell wall.

The extracellular matrix (ECM) of animal cells functions in support, adhesion, movement, and regulation

The extracellular matrix (ECM) of animal cells functions in support, adhesion, movement, and regulation • In many cells, fibronectins in the ECM connect to integrins, intrinsic membrane proteins.

. Intracellular junctions help integrate cells into higher levels of structure and function •

. Intracellular junctions help integrate cells into higher levels of structure and function • Plant cells are perforated with plasmodesmata, channels allowing cysotol to pass between cells.

Animal have 3 main types of intercellular links: tight junctions, desmosomes, and gap junctions

Animal have 3 main types of intercellular links: tight junctions, desmosomes, and gap junctions • In tight junctions, membranes of adjacent cells are fused, forming continuous belts around cells. – This prevents leakage of extracellular fluid.

Desmosomes (or anchoring junctions) fasten cells together into strong sheets, much like rivets. •

Desmosomes (or anchoring junctions) fasten cells together into strong sheets, much like rivets. • Gap junctions (or communicating junctions) provide cytoplasmic channels between adjacent cells.

Microtubules • In many cells, microtubules grow out from a centrosome near the nucleus.

Microtubules • In many cells, microtubules grow out from a centrosome near the nucleus. • In animal cells, the centrosome has a pair of centrioles, each with nine triplets of microtubules arranged in a ring. • During cell division the centrioles replicate.