Water structures carrying DNA information Application to HIVAIDS

Water structures carrying DNA information, Application to HIV/AIDS and Autism L. Montagnier, Borovets, October 23, 2013

The Evolution of Medicine Toward 4 P Qualities • P 1 Preventive • P 2 Predictive • P 3 Personalized • P 4 Participative

Emergence or reemergence of new epidemics due to : ü Globalization of exchanges and travels ü Demography: concentration in large cities ü Nutrition (pesticides, water) ü Environmental factors ü Climate changes, electromagnetic radiations ü Contacts with wild and farm animals ü Decline of immune defenses

The most important burden Chronic Diseases - Cancers - Cardiovascular - Neurodegenerative - Arthritic - Autoimmune - Multifactorial, but in common: oxidative stress infectious agents (? )

Various environmental factors effects accumulate Radiations ( , X, UV, visible, hertzian) + + + + + Air chemical pollution Inadequate Food Excessive physical exercize tobacco smoke alcohol ischemia Parasitic infections Bacterial infections Viral infections = DISEASE

Pathological effects of Oxydative Stress Lipid hyperoxydation (plasma, cell membranes) Protein Oxydation --> destruction, agregates DNA oxydation --> Mutations, Chromosomal Breakage

Oxidative stress Weakens the immune system Activates transcription factors (NF-kappa B) Activates genes involved in cell division, inflammatory cytokines, lymphocytes activation Immune dysfunction, apoptosis (TH 1 TH 2)

The Human Genome Genes (+ introns +promoters) Retroelements: retroviruses retrotransposons LTR Total 3% 8% 13% 23% 47% Repeats 50% Unknown LINE SINE 22% 13% ?

Our microbial ecosystem : Mucosa Skin Constant exposure to microbial agents and immune protection

Extreme genetic plasticity of microorganisms: ü virus : HIV, Influenza ü Bacteria (plasmids) ü Parasites (retrotransposons) against reaction of the immune system

How bacteria have learned how to persist despite the immune system - Biofilms - Mobile antibiotic resistant genes - Nanoforms - Nanostructures - Endosymbiontes

Parasite Bacterium Virus (Worm) intracellular Filarial nematode Wolbachia

Persistent cold infections • Inability of the immune system to eradicate them (tolerance, oxidative stress) • Non-multiplicative forms of « classical » bacteria • intracellular bacteria • Sanctuaries (bone marrow, joints, intestine, brain, …. ) • Vectors (Parasites) • Remote effects (toxin, nanostructures) Antibiotics affect them only when they go out of sanctuaries

EXPLORING THE ROLE OF LATENT INFECTION IN CHRONIC DISEASES A physical and molecular approach

Two technologies for detecting bacteria and viral DNA’s • A new technology based on the production of electromagnetic waves • The Polymerase Chain Reaction (PCR) using the 16 S ribosomal DNA variability

Classical model of PCR oligo Taq polymerase oligo

A newly discovered property of DNA : Resonance emission of low frequency electromagnetic waves by high water dilutions of DNA.

Molecular recognition without close contact (by waves and resonance)

Capture of the signals Sample X 500 Sensor coil Amplifier Signal Analysis software Computer

FACTS Detection of Ultra Low Frequencies Waves (ULF 500 -2000 hertz) in certain dilutions of filtrates (100 n. M, 20 n. M, 15 n. M) from cultures of microorganisms (virus, bacteria) or from the plasma of humans infected with the same agents.

7 -100 Hz Filtrati on 0. 1 0. 02 2 ng/1 ml 10 -2 10 -3 10 -4 10 -5 10 -6 10 -7 10 -8 10 -9 10 -10 10 -12 10 -13 10 -14 10 -15 1016 1000 3000 Hz

Amplitude Noise (+) Time (sec)

Spectral Frequency Analysis Fourier Tranformation A positive signal is defined by: üamplitude increase üShift to higher frequencies (500 -2000 Hertz)

Noise Tim e (s ec) (+) Frequency (1 -20000 Hertz)

Suspensions of pure culture of bacteria with pathogenic potential are producing electromagnetic signals (EMS) in the range of dilution usually 10 -8 10 -13, sometime (E. coli) up to 10 -18 They are: E. coli, Streptococcus, Salmonella, Staphylococcus, B. subtilis, Clostridium, Pseudomonas, etc.

Micro-organisms involved in EMS induction 1. DNA from main pathogenic bacteria Streptococcus Staphylococcus Pseudomonas Mycoplasma pirum Salmonella Clostridium Proteus mirabilis B. Subtilis Borrelia burgdorferi Sutterella - From viruses - Genes involved HIV 1 Influenza group A HBV HCV M. pirum adhesin HIV genes

Interpretation 10 -2 DNA Water excitated state crystal like gel no EMS 10 -9 Free polymer self maintained by EMS

I – DNA’s emit EMS II – EMS are produced by water nanostructures (naneons) III – EMS are producing naneons IV – Naneons and EMS carry specific DNA information

EMS are produced by water nanostructures (naneons) Evidence : from filtration ü Size : between 20 and 100 n. M for bacterial sequences ü Smaller that 20µM for viral sequences from biophysical studies ü indicating spectrometral changes in the dilutions producing EMS

Naneons and EMS carry specific DNA information Natural and digital transmission

Classical model of PCR oligo Taq polymerase oligo

PCR on water nanostructures oligo Taq EMS oligo

DNA Water Naneons EMS Water Naneons 7 Hz PCR DNA

Generator 7 Hz DNA -6 -6 water Tube 1 Tube 2 µmetal -2 -3 -4 -5 -6 18 hrs water + + + EMS

D-4 LTR HIV DNA (104 bp) 7 Hz, 18 Hrs and then PCR (35 cycles) from D-2 to D-15 after filtration 450 and 20 n. M Transmission in water of D-4 LTR HIV DNA (104 bp) 7 Hz, 18 Hrs and then PCR (35 cycles) from D-2 to D-15 after filtration 450 and 20 n. M DW: Distilled Water / FD 2: Dilution 10 -2 after filtration 450 and 20 n. M

Water-mediated photonic transmission of DNA Water Naneons EMS Computer Digitized Receiver EMS Analog PCR Computer Water Naneons DNA

Reproduction of DNA transduction in other laboratories File EMS of 194 bp DNA from HIV 1 LTR Sent to Benevento University, Molecular Biology Laboratory DNA reproduced and sequenced 100 % identical to original File EMS of 499 bp DNA from Borrelia burgdorferi Sent to Laboratory of Chronix Biomedicals University of Gottingen

Molecular Biology Laboratory DNA reproduced and sequenced 100 % identical to original File EMS of 499 bp DNA from Borrelia burgdorferi Sent to Laboratory of Chronix Biomedicals University of Gottingen

Water-mediated photonic transmission of DNA Gel electrophoresis of the PCR DNA product (Borrelia Burgdorferi) E. Schutz et al. Goettingen, 2011

ELF DNA WATER (plasma) DNA DNA How pathogenic information can persist, and escape immune defence and treatment.

Two main problems for AIDS research in 2013 - The viral DNA reservoir - The Higher sensitivity of African people to HIV infection

The dual origin of EMS in HIV/AIDS EMS plasma RBC HIV < 20 n. M ++ +/Agent X < 100 n. M - ++

Evidence that the agent X is a bacterium - Presence of a sequence close to 16 S ribosomal DNA of Rickettsiales - Sensitive to antibiotics ( in vitro and in vivo) - Growth in cell line - Electron microscopy - Associated with Red Blood Cells

Human sequences associated with the agent X 237 bp (human chromosome 1) 213 bp (human chromosome 7) and more…

Strong correlation with HIV infection - Contains human DNA sequences emitting EMS only in pathogenic HIV infection -Sequence 213 bp from Chromosome 7 EMS in HIV + patients no EMS in HIV - patients

DNA HIV plasma RBC Risk factor 2 RBC Risk factor 1 RBC PCR EMS (LTR 195 bp) (all HIV) (20 n. M) + + + - (100 n. M) (213/237 bp) ( all HIV+, a few HIV-) (HIV+) ( all HIV+, many HIV-)(HIV- & HIV+) (100 n. M) RISK FACTORS OF HIV INFECTION

CONCLUSION 1 « A new intracellular bacterium has been found in human red blood cells. A variant bearing extra DNA sequences is present in all HIV infected patients and also in some healthy HIV negative African individuals”.

CONCLUSION 2 Origin of EMS : not necessarily depending on DNA sequence - depending on transmissible modification of DNA (free radicals of water ? ) - Association with pathogenicity

AUTISM : THE GUT, BLOOD and BRAIN MICROBIAL CONNECTION

GUT BLOOD BRAIN

% EMS/noise Correlation between EMS, antibiotic treatment and clinical signs in an autistic child 40 CEFUROXIM 30 - 20 - Starts speaking Accept various food More present with Family members AZITHROMYCIN 10 - 0 - 27 50 100 133 days

Environment al factors genetic susceptibility Oxidative stress immunosuppres Bacterial sion agents Oxidative Somatic stress mutations « prion » effect Reversible Less and less reversible

In press Electromagnetic detection of HIV DNA in the blood of AIDS patients treated by antiretroviral therapy. Luc MONTAGNIER†×^, Jamal AISSA†, Claude LAVALLEE×, Mireille MBAMY⁰, Joseph VARON#, and Henri CHENAL⁰ × World Foundation for AIDS research and Prevention (UNESCO), 1 rue Miollis, 75015, Paris, France † Nanectis Biotechnologies, France ⁰ CIRBA Centre Intégré de Recherches Biocliniques d’Abidjan, Ivory Coast # The University of Texas Health Science Center, Houston, USA. Abstract : Electromagnetic signals of low frequency have been shown to be durably produced in aqueous dilutions of the Human Imunodeficiency Virus DNA. In vivo, HIV DNA signals are detected only in patients previously treated by antiretroviral therapy and having no detectable viral RNA copies in their blood. We suggest that the treatment of AIDS patients pushes the virus towards a new mode of replication implying only DNA, thus forming a reservoir insensitive to retroviral inhibitors. Implications for new approaches aimed at eradicating HIV infection are discussed. Key words: DNA, Electromagnetic signals, bacteria

Centre Intégré de Recherche Bioclinique d’Abidjan

World Foundation of AIDS Research and Prevention R. Olivier, Cl. Lavallee, H. Chenal, M. Mbamy, Nanectis Biotechnologies SA J. Aissa, Cl. Lavallee, R. Olivier Goettingen University and Chronix Biomedicals E. Schutz, H. Urnovitz University of Washington, Seattle Gerald H. Pollack

“Absence of evidence is not evidence of absence” Carl Sagan