Chromatography 1903 Mikhail Tswett Chromatography Adsorption Partition Ion

ﺍﻟﺒﺪﺍﻳﺔ Chromatography 1903 ﻋﺎﻡ Mikhail Tswett

ﺗﻘﺴﻴﻢ ﺍﻟﻜﺮﻭﻣﺎﺗﻮﺟﺮﺍﻓﻲ Chromatography Adsorption Partition Ion Exchange Exclusion GCs, LC, CC, PC, TLC SFC IC CZE, GPC Affinity CE

ﺍﻻﺳﺘﺨﻼﺹ ﺃﺤﺪﺙ ﻧﻈﻢ EXTRACTION 8 Supercritical Fluid Ext. (SFE * Microwave Solvent Ext. (MSE) * Accelerated solvent Ext. (ASE) * Sonication Ext. (SAE) * Solid Phase Ext. (SPE) * Pressurized Solvent Ext. (PSE) * Purge trap & Purge Headspace *EPA & FDA

ﺃﺤﺪﺙ ﻧﻈﻢ ﺍﻟﺘﻘﺪﻳﺮ ﻭﺍﻟﺘﺄﻜﻴﺪ * SFC * GC/ECD-NPD-FID-TCD-FPD…. * GC/ECD-ECD, NPD-NPD, FPD-FPD * LC/MS, LC/MS-MS * ELISA * CE, CEC * FTIR # GC/ MS, GC/MS-MS # LC/ MS, LC/MS-MS

GC

SFC/UVD-ECD-FPD

ELISA THEORY

ELISA PLATE

HPLC/UV/FL

75 ﻷﻜﺜﺮ ﻣﻦ ﻣﺒﻴﺪ ﺍﻻﺳﺘﺮﺟﺎﻉ 8 Pyrethroids 8 Herbicides 7 Fungicides 12 Carbamates (SFC) 8 Carbamates(HPLC) 16 OCPs 25 OPPs ﺍﺳﺘﺨﻼﺻﻬﻢ ﻭﺗﻘﺪﻳﺮﻫﻢ ﺑﻨﻔﺲ ﺍﻟﻄﺮﻕ ﻣﻌﺪﻝ 96 -103%. n 95 -102% n 94 -101% n 98 -103% n 88 -103% n 99 -109% n 98 – 106% n ﻣﺒﻴﺪﺍ ﻳﻤﻜﻦ 16 ﺗﻢ ﺍﺿﺎﻓﺔ n

ﺍﻟﺰﻣﻦ Supercritical Fluid Ext. (SFE) 15. 0 min Microwave Solvent Ext. (MSE) 5. 0 Accelerated solvent Ext. (ASE) 20. 0 Sonication Ext. (SE) 10. 0 Solid Phase Ext. (SPE) 15. 0 Pressurized Solvent Ext. (PSE) 15. 0 Purge trap & Purge Headspace 25. 0 10. 0 * SFC * ELISA 2. 0 * GCs 30. 0 * HPLC 50. 0 * LC/MS 40. 0 ú * GC-MS 45. 0 ú * CE 22. 0

Atrazine residues (ppb) & RSD in frozen samples & strawberry jam.

Acrylamide Method Limit of Specificit Percent Quantitatio y CV n GC/MS (Underivatized) 10 ppb High 10 GC/MS (Derivatized) 50 ppb High 5 -10 LC/MS 20 ppb High 5 -10 * LC/MS/MS 10 ppb High 5 -10

PYRETHROID RESIDUS / SFC ppm SFE Cyperm. Khullas. Sukkari N. Seif MSE Deltam. Cyperm. Deltam. 0. 02 0. 01 0. 03 0. 02 ND 0. 02 0. 01 ND ND 0. 03 0. 02 ND 0. 02 0. 01 ND

ACARICIDE RESIDUES / SFC ppm SFE Amitraz MSE 0. 05 -0. 08 0. 04 -0. 07 cofol 0. 05 -0. 09 Tourk Abamectin 0. 04 -0. 07 0. 06 -0. 09 0. 06 -0. 08 0. 05 -0. 06 -0. 08

OCP RESIDUES/GC-ECD ppm SFE Lidane. MSE Dield. Lindane. Dield. Khullas 0. 11 0. 06 0. 13 0. 06 0. 12 0. 03 0. 15 0. 01 Sukkari 0. 16 0. 04 0. 16 0. 08 0. 18 0. 04 0. 17 0. 05 N. Seif 0. 14 0. 08 0. 15 0. 03 0. 16 0. 02 0. 16 0. 08

CONCLUSION NEW EXTRACTION METHODOLOGY BY SFE, ASE, SONICATION, SPE &MSE WAS SUCCESSFUL FOR MORE THAN 90 PESTICIDE RESIDUES IN FOODS. NEW DETERMIATION METHODOLOGY BY SFC, HPLC& GC WAS SUCCESSFUL FOR 90 PESTICIDE RESID UES IN FOODS. APPLICATION OF NEW METHODOLOGY OBTAINED TO PROTECT THE FOOD CUSTOMERS.

* ELISA techniques coupled with SFE & n MSE is less time consuming, high recovery% and more sensitive with lower detection limit of Pesticide residues. * New techniques very applicable with Foods, Waters, Soils, Air and others.

RECOMENDATIONS MODERN UNITS AND LABS WITH NEW TECH. FOR RESEARCHES n DATA BASE n MARKETS n ANALYTICAL QC/QA ACCRED. n PESTICIDE RESIDUE MAPS. n n