Types of Liquid Chromatography I Ion Exchange Chromatography
- Slides: 20
Types of Liquid Chromatography I. Ion Exchange Chromatography A. Factors influencing retention B. Suppressed ion exchange II. Partitioning Chromatography A. Normal phase/ reverse phase III. Size Exclusion Chromatography IV. Supercritical Fluid Chromatography/ SFE V. Capillary Electrophoresis
Factors Influencing retention in Ion Exchange • Ionic strength: not real important in selectivity • p. H: anion exchange p. H % retention % cation exchange p. H % retention & • Temperature: T % efficiency % Flow rate: Slightly slower than other HPLC methods to maximize resolution & improve mass transfer kinetics • Buffer salt: Influences p. H & selectivity • Organic Modifier: Solvent strength increases with increases in modifier
Suppressed Ion Chromatography
Partitioning Chromatography • Analyte interacts with mobile and stationary phase, differential interaction leads to selectivity • Interactions that are important – Proton accepting ability * most important – Dipole interaction – Proton Donor * most important – e- pair donating ability – Van der Waals dispersion forces
Types of Partitioning Chromatography Normal Phase Stationary phase: Polar with short carbon chains Mobile phase: Non-polar such as hexane Polar things are retained on column Applications: oil soluble vitamins, nitrophenols Example Stationary Phase
Types of Partitioning Chromatography REVERSE PHASE More common Stationary Phase: Hydrophobic C 18 or C 8 Mobile Phase: Polar usually aqueous Polar substance elute first
Solvophobic Theory • Water has a lot of intermolecular interactions in the liquid phase • Solute dissolved in water disrupts those intermolecular interactions • Solute is forced out of aqueous phase not because of favorable interactions between analyte and stationary phase but because of unfavorable interactions between solute and water when solute is dissolved in aqueous phase hence: SOLVOPHOBIC THEORY • Polar functional groups such as –OH would increase the favorability of interaction and thus decrease retention (in mobile phase longer) • Polar things elute non-polar things elute
Sample Column Mobile Packing phase C-18 C-2
Size Exclusion Chromatography • Molecules partition into bead • Large molecules can’t get in and are unretained, small molecules get in and never get out, medium size will differentiate • Need at least 10% difference in MW to differentiate • GPC organics • Gel filtration chromatography aqueous
Size 1) Short, well defined retention times Exclusion Chromatography 2) Narrow bands high sensitivity 3) No sample loss b/c no interaction with stat. phase 4) No column destruction b/c no interaction with stat. phase
Size Exclusion Chromatography Disadvantages 1) Only limited # of peaks can be separated b/c time scale of separation is short 2) Not good for separating compounds of similar size
Summary Phase/ Mode Reverse phase Normal phase Ion Exchange Size Exclusion Chiral Hydrophobic % Use 50. 6 24. 1 14. 1 6. 6 3. 5 1. 1
Supercritical Fluids
Supercritical Fluid Chromatography Instrumentation
Properties of Mobile Phases Used in Chromatography Mobile Density (g/m. L) Phase Gas 0. 6 – 2. 0 x 10 -3 Super- 0. 2 – 0. 9 critical fluid liquid 0. 8 – 1. 0 Viscosity (poise 10 -4) 0. 5 – 3. 5 Diffusion coefficient (cm 2/sec) 0. 01 – 1. 0 2. 0 – 9. 9 0. 5 – 3. 3 x 10 -4 30 -240 0. 5 – 2. 0 x 10 -5
Fluid Dipole Tc(o. C) momen t Pc(atm) Density c(g/m. L) 400(g/m L) CO 2 N 2 O NH 3 N-C 5 N-C 4 SF 6 Xe CCl 2 F 2 CHF 3 0 0. 51 1. 65 0 0 0. 17 1. 47 72. 9 72. 5 112. 5 33. 3 37. 5 37. 1 58. 4 40. 7 46. 9 31. 3 36. 5 132. 5 196. 6 152. 0 45. 5 16. 6 111. 8 25. 9 0. 47 0. 45 0. 24 0. 23 0. 74 1. 10 0. 56 0. 52 0. 96 0. 94 0. 40 0. 51 0. 50 1. 61 2. 30 1. 12
Supercritical fluid extraction (SFE) Used instead of soxhlet extraction Advantages 1. Fast: rate of diffusion between sample matrix & extraction 3. Less Harmful solvent fluid 10 -60 min vs. 4. Many SCF are gases at days RT, recovery of analytes 2. Solvent strength can is easy be varied by 5. Many SCF are cheap, changes in P & T inert, and non-toxic 6. On-line extraction
Supercritical fluid extraction • Disadvantages 1. Method development is more complex 2. Limited # of mobile phases 3. Capital equipment & CO 2 expensive 4. Requires more operator time to do 1 at time
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