Ch 12 Section 3 DNA RNA and Protein
- Slides: 40
Ch. 12 Section 3: DNA, RNA, and Protein MAIN IDEA: DNA CODES FOR RNA, WHICH GUIDES PROTEIN SYNTHESIS
The Central Dogma The flow of genetic information is from DNA, to RNA, to Proteins directly cause outward physical traits
Protein Synthesis 2 Steps: Transcription: synthesis of m. RNA complementary to a specific part of the DNA Translation: m. RNA code is read and translated to build a specific protein
RNA: Ribonucleic Acid 2 nd type of nucleic acid (What’s the 1 st? ) Made up of nucleotides Phosphate 5 -C 1 Sugar – Ribose of 4 nitrogenous bases
Nitrogenous Bases A, G, C, U U = Uracil (Replaces Thymine) Base-Pairing A-U, Rules C-G Function: to transfer genetic material (in the nucleus) to site of protein synthesis (in the cytoplasm)
Differences from DNA Different sugars – ribose vs. deoxyribose Different bases – A, U, C, G vs. A, T, C, G Different structure – RNA=single-stranded vs. DNA=double-stranded
Types of RNA Messenger RNA (m. RNA) – carries genetic information from nucleus to cytoplasm Transfer RNA (t. RNA) – carries specific amino acids to the ribosome to assemble a protein Ribosomal RNA (r. RNA) – major component of ribosome (protein factory)
How is RNA made? Transcription – process by which RNA is made complementary to a specific segment of DNA (gene) in the nucleus
Steps of Transcription RNA Polymerase binds to promoter segment of DNA unwinds and separates RNA Polymerase adds complementary RNA nucleotides to the 3’ end making m. RNA (SAME DIRECTION AS DNA POLYMERASE!) Process ends when RNA Polymerase reached termination sequence on DNA
RNA Processing Once transcription ends, the m. RNA is processed further Introns Exons – non-coding sequences are removed from the m. RNA – leftover sequences are spliced back together Additional modifications made – 5’ cap and poly-A tail Final m. RNA leaves the nucleus through nuclear pores to be translated
Protein Synthesis Proteins carry out genetic instructions contained in DNA Amount and type of proteins determine cell’s structure and function
Protein Synthesis Proteins are assembled at the ribosome (in the cytoplasm) Amino acids link together via peptide bonds (polypeptide) Sequence of amino acids determine protein’s structure and function
The Code Correlation between the nucleotide sequence of DNA and amino acid sequence of protein Codon – combination of 3 m. RNA nucleotides that code for a specific amino acid 20 different AA’s, but MILLIONS of types of proteins
Codons 64 different possible codons – 20 amino acids What does this mean? Start Codon (AUG) – starts translation Stop Codons (UGA, UAG) – stops translation
Translation The process of assembling a polypeptide (protein) from a nucleotide sequence (m. RNA) Essentially, “translating” from one language (nucleotides) to another language (proteins)
Steps to Translation Amino acids are put together from information encoded on the m. RNA As m. RNA moves through the ribosome, t. RNA’s add a specific amino acid to the growing polypeptide chain Process continues until a Stop codon is reached, and the polypeptide is released Each t. RNA has a complementary nucleotide sequence to the m. RNA codon (called an anticodon)
Let’s Watch The Process… https: //www. youtube. com/watch? v=g. G 7 u. Csk. UOr. A
SEQUENCE IS KEY! THE SEQUENCE OF AMINO ACIDS IN A POLYPEPTIDE DETERMINES THE STRUCTURE AND FUNCTION OF THE FINAL PROTEIN!!! CHANGE IN SEQUENCE = CHANGE IN PROTEIN
One Gene, One Enzyme Each gene located on a chromosome codes for one, and only one, protein
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