Photosynthesis Solar Power Photoautotrophs Photosynthetic organisms plants algae
- Slides: 53
Photosynthesis Solar Power
Photoautotrophs Photosynthetic organisms (plants, algae and some bacteria) make their own food from light and CO 2 Mostly occurs in chloroplasts of the leaf cells (spongy mesophyll)
Chloroplasts Found in all green parts Most abundant in mesophyll cells Chlorophyll is a green pigment which give plants their color
Chloroplast Structure • Thylakoids are membrane-bound discs in the chloroplast • Outside fluid is called the stroma • Contained in a double membrane
Chloroplast x 5, 755
Basic Chemical Equation 6 CO 2 + 12 H 2 O + Light Energy → C 6 H 12 O 6 + 6 O 2 + 6 H 2 O Or CO 2 + H 2 O → CH 2 O + O 2 Carbon Dioxide + Water + Light Carbohydrates + Oxygen
Water is Split and Reformed Page 171 of your book describes the discovery process (interesting read) The water in the products is not the same water! 6 C O 2 + 12 H 2 O ↓ C 6 H 12 O 6 + 6 H 2 O + 6 O 2
Oxidation/Reduction An oxidation reaction removes electrons from an atom A reduction reaction adds electrons to an atom H H | | HO-C-OH → O=C=O → HO-C-OH | | H H (20 electrons) (16 electrons)
OIL RIG Oxidation is Loss Reduction is Gain (of electrons) (reducing the charge)
Reduction Process Splitting of water releases electrons and hydrogen ions These bond to the carbon, reducing it to a sugar Electrons increase the potential energy of the molecule ∆G is + (endergonic) Light provides the extra free energy
Steps of Photosynthesis Light Reactions Sunlight harvested to create ATP and NADPH Calvin Cycle ATP and NADPH turn CO 2 into sugar
The Basics of Light = electromagnetic energy Shorter the wavelength, the higher the energy Chlorophyll absorbs the energy contained in light
Part 1: The Light Reactions
Phase 1: Excitation of Electrons
How Chlorophyll Absorbs Light The electron in chlorophyll gets excited and jumps to a higher orbital When it falls back into its normal orbital, it releases light energy This can then energize the molecule next to it Step 1 Light light
Light and heat! High free energy = unstable Energy is spontaneously given off as heat and light
These electrons can be excited by energy from the sun
Photosystems Step 1 Chlorophyll is arranged in photosystems Hundreds of chlorophyll molecules and proteins All molecules “pass their energy” to one particular chlorophyll Called the reaction center
The Primary Electron Acceptor When the reaction center chlorophyll molecule's electron is excited it passes to the primary electron acceptor Does not fall back down Step 1
Photsystem Energy! e- Energy! ee- To Primary Electron Acceptor e- e- e- Energy! e- e- e-
Photosystem I and II Have slightly different reaction centers and absorb slightly different wavelengths of light Located in the thylakoid membrane Chlorophyll is the same! Protein arrangement around the chlorophyll is different
Phase 2: Electron Flow generates ATP and NADPH 2 A Noncyclic Electron Flow 2 B Cyclic Electron Flow
2 A Non-cyclic electron flow The predominate route Electron from photosystem II transferred to photosystem I ATP is made during this process Electron ends up on NADP+ molecule, forming NADPH Water is split, replacing the electron of the Reaction center
Step 1 Photosystem II absorbs light Energy passed to reaction center Electron is excited Electron is passed to primary electron receptor Reaction center has an electron hole
Step 2 Enzyme splits water 2 H 2 O → 4 H+ + O 2 + 4 e- Electrons replace the reaction center electron Forms O 2
Splitting of Water H e e H e O e e e H H e e e O e H e e e H
Step 3 Each electron is passed down an Electron Transport Chain to Photosystem I ATP made via chemiosmosis!
Step 4 Electrons “falling” down the chain allow proteins to pump H+ ions into thylakoid space Buildup of H+ in thylakoid space H+ ions diffuse through ATP synthase
Chemiosmosis Electrons falling down the chain is coupled with pumping H+ into the thylakoid space (innermost part of thylakoids) This causes the concentration of H+ in the thylakoid to be much higher than in the stroma H+ ions cannot simply pass through the membrane Instead they diffuse through ATP Synthase which uses the energy of the concentration gradient to produce ATP
Step 5 Electron ends up in the reaction center of Photosystem I Replaces an electron that is passed to the primary electron acceptor
Step 6 The primary electron acceptor of photosystem I passes electrons to NADP+ forming NADPH No ATP is made
Note this diagram uses a different set of numbers
H+ Light H+ H+ e. HH+2 OO 2 e- NADPH
Noncyclic Electron Flow Review http: //trc. ucdavis. edu/biosci 10 v/bis 10 v/media/ch 05/noncyclic_v 2. html http: //www. youtube. com/watch? v=e. Y 1 Reqi. Yw. Ys Sunlight energizes electrons, which drive the synthesis of ATP as they are passed from photosystem II to photosystem I They are again excited in photosystem I and transferred to NADP+ to form NADPH and ATP are used in the Calvin cycle to make sugar
Review of Light Reactions http: //www. youtube. com/watch? v=Q_1 mx. Zd. F 2 TY&feature=video_response
2 B Cyclic Electron Flow Non-cyclic electron flow creates equal amounts of ATP and NADPH but the Calvin cycle requires more ATP than NADPH So electrons are sometimes rerouted back to the ETC from photosystem I to produce more ATP No NADPH is produced, nor is water split
e- No water split! No O 2 made No NADPH made
Review of Light Reactions Light is harnessed by chlorophyll pigments in chloroplast's thylakoid membranes This energy is used to create NADPH and ATP via Non Cyclic Electron Flow Water is split and O 2 is released as a byproduct Extra ATP is made via Cyclic Electron Flow The ATP and NADPH is used in the Calvin Cycle
DNA • Make a list of everything needed in the light reactions of photosynthesis
Light Reaction Requirements • • Chloroplast/ thylakoid membrane Chlorophyll / photosystems H 2 O – provides electrons Light – provides energy ETC / NADP+ reductase NADP+ / ADP + Pi ATP Synthase H+
The Two Steps
Part II: The Calvin Cycle
Overview Takes place in stroma of chloroplast Uses CO 2 and energy from NADPH and ATP to make sugar Actual product is glyceraldehyde 3 phosphate (G 3 P) G 3 P can then form glucose and other sugars 3 CO 2 + 9 ATP + 6 NADPH → 1 G 3 P + 9 ADP + 9 Pi + 6 NADP+
Review of Calvin Cycle http: //www. science. smith. edu/departments/Bio logy/Bio 231/calvin. html http: //www. youtube. com/watch? v=OYSD 1 j. OD 1 d. Q
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- What are photoautotrophs
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- Unlike photoautotrophs chemoautotrophs obtain energy
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- Organism
- Diatoms unicellular or multicellular
- Algae vs plants
- Terrestrial plant
- What is a photosynthetic organism
- Euglena and volvox
- Photosynthetic apparatus
- Chloroplast contain saclike photosynthetic membranes called
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- Photosynthetic cells
- Photosynthetic autotroph
- What kingdom is photosynthetic aquatic and unicellular
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- Objectives of photosynthesis
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