Principles and Techniques of Instrument Processing and Sterilization

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Principles and Techniques of Instrument Processing and Sterilization Chapter 21 Copyright © 2009, 2006

Principles and Techniques of Instrument Processing and Sterilization Chapter 21 Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Chapter 21 Lesson 21. 1 Copyright © 2009, 2006 by Saunders, an imprint of

Chapter 21 Lesson 21. 1 Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Learning Objectives Pronounce, define, and spell the Key Terms. Discuss the seven steps in

Learning Objectives Pronounce, define, and spell the Key Terms. Discuss the seven steps in processing dental instruments. Describe the classification of instruments used to determine the type of processing. Describe the Centers for Disease Control and Prevention (CDC) guidelines for the sterilization and disinfection of patient-care items. (Cont’d) Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Learning Objectives (Cont’d) Describe the CDC guidelines for cleaning and decontaminating instruments. Explain the

Learning Objectives (Cont’d) Describe the CDC guidelines for cleaning and decontaminating instruments. Explain the purpose of a holding solution. Understand the safety precautions necessary in the operation of an ultrasonic cleaner. Describe the precautions necessary in the packaging of materials for sterilization. Describe the CDC guidelines for the preparation and packaging of instruments for sterilization. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Introduction One of the most important responsibilities of the dental assistant is to process

Introduction One of the most important responsibilities of the dental assistant is to process contaminated instruments for reuse. Instrument processing involves much more than sterilization. Sterilization is a process intended to kill all microorganisms and is the highest level of microbial destruction. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

CDC Guidelines for Instrument-processing Area Copyright © 2009, 2006 by Saunders, an imprint of

CDC Guidelines for Instrument-processing Area Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Instrument Processing Instrument processing involves much more than sterilization. Proper processing of contaminated dental

Instrument Processing Instrument processing involves much more than sterilization. Proper processing of contaminated dental instruments is actually a seven-step process. Although the seven steps are not difficult to learn, it is very important for you to have a clear understanding of how and why each step is performed. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Terms Used in Instrument Processing Copyright © 2009, 2006 by Saunders, an imprint of

Terms Used in Instrument Processing Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Classification of Patient-Care Items Patient-care items are categorized into three classifications: Critical Ø Semicritical

Classification of Patient-Care Items Patient-care items are categorized into three classifications: Critical Ø Semicritical Ø Noncritical Ø (Cont’d) Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Classification of Patient-Care Items (Cont’d) The categories are based on the risk of infection

Classification of Patient-Care Items (Cont’d) The categories are based on the risk of infection associated with their intended use. The classifications are used to determine the minimal type of posttreatment processing. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Critical Instruments Critical instruments are items used to penetrate soft tissue or bone. They

Critical Instruments Critical instruments are items used to penetrate soft tissue or bone. They have the greatest risk of transmitting infection and should be sterilized with the use of heat. Examples of critical instruments include forceps, scalpels, bone chisels, scalers, and burs. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Semicritical Instruments Semicritical items instruments touch mucous membranes or nonintact skin and have a

Semicritical Instruments Semicritical items instruments touch mucous membranes or nonintact skin and have a lower risk of disease transmission. The majority of semicritical items in dentistry are heat-tolerant, and they should also be sterilized. If the item will be damaged by heat, it should undergo, at a minimum, high-level disinfection. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Noncritical Instruments Noncritical instruments pose the least risk of transmission of infection because they

Noncritical Instruments Noncritical instruments pose the least risk of transmission of infection because they come into contact only with intact skin, which is an effective barrier to microorganisms. These items should be cleaned and processed with the use of an EPA-registered intermediate-level or low-level disinfectant after each patient use. Noncritical clinical devices include the position indicator device of the x-ray unit tube head, the lead apron, and the curing light that comes into contact only with intact skin. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Personal Protective Equipment For disease agents from a previous patient to be prevented from

Personal Protective Equipment For disease agents from a previous patient to be prevented from being transferred to yourself, another dental-team member, or the next patient, instrument processing must be performed in a consistent and disciplined manner. You must always use personal protective equipment (PPE), including utility gloves, mask, eyewear, and protective clothing, when processing instruments. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Fig. 21 -1 PPE must be worn during preparation of instruments for sterilization. Copyright

Fig. 21 -1 PPE must be worn during preparation of instruments for sterilization. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Transporting and Processing Contaminated Patient-Care Items The dental assistant may be exposed to microorganisms

Transporting and Processing Contaminated Patient-Care Items The dental assistant may be exposed to microorganisms through contact with contaminated instruments or other patientcare items. Exposure may occur through percutaneous injury (e. g. , needlesticks, cuts) or contact with the mucous membranes of the eyes, nose, or mouth. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Instrument-Processing Area The instrument-processing area, or sterilization area, should be centrally located in the

Instrument-Processing Area The instrument-processing area, or sterilization area, should be centrally located in the office to permit easy access from all patient-care areas. A central location minimizes the need to carry contaminated items through clean areas of the office (where sterilized instruments, fresh disposable supplies, and prepared trays are stored). (Cont’d) Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Instrument-Processing Area (Cont’d) The ideal instrument-processing area: Should be dedicated only to instrument processing

Instrument-Processing Area (Cont’d) The ideal instrument-processing area: Should be dedicated only to instrument processing Ø Should be physically separated from the operatories and dental laboratory Ø Should not be a part of a common walkway Ø The area should not have a door or windows that open to the outside, because dust may enter the area. (Cont’d) Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Instrument Processing Area (Cont’d) The processing area should have good air circulation to control

Instrument Processing Area (Cont’d) The processing area should have good air circulation to control the heat generated by the sterilizers. The size of the area should accommodate all the equipment and supplies necessary for instrument processing, with multiple outlets and proper lighting, water, and air and vacuum lines for flushing of high-speed handpieces. (Cont’d) Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Instrument Processing Area (Cont’d) A deep sink should have hands-free controls for instrument rinsing

Instrument Processing Area (Cont’d) A deep sink should have hands-free controls for instrument rinsing and (if space permits) a foot-operated or other hands-free trash receptacle. The flooring should be an uncarpeted, seamless, hard surface. The size, shape, and accessories of the instrument- processing area vary among dental offices. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Rankings of Evidence Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc.

Rankings of Evidence Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Workflow Pattern Regardless of the size or shape of the instrumentprocessing area, four basic

Workflow Pattern Regardless of the size or shape of the instrumentprocessing area, four basic areas govern the pattern of workflow. Processing of instruments should proceed in a single loop, from dirty to clean to sterile to storage, without ever “doubling back. ” If the instrument-processing area is small, you can use signs: Ø Ø Ø “Contaminated items only” “Precleaning area” “Cleaned items only” “Sterile items only” “Sterilization area” Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Fig. 21 -2 A, Linear and B, U-shaped instrument-processing areas. Copyright © 2009, 2006

Fig. 21 -2 A, Linear and B, U-shaped instrument-processing areas. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Contaminated Area All soiled instruments are brought into the contaminated area, the initial receiving

Contaminated Area All soiled instruments are brought into the contaminated area, the initial receiving area, where they are held for processing. Any disposable items that have not already been discarded in the treatment room are removed from the instrument tray and disposed of as contaminated waste. Thorough cleaning should be done before all disinfection and sterilization processes. It should involve the removal of all debris and organic materials (e. g. , blood and saliva). The contaminated area contains clean protective eyewear and utility gloves, counter space, a sink, a waste-disposal container, holding solution, an ultrasonic cleaner, an eyewash station, and supplies for the wrapping of instruments before sterilization. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Fig. 21 -3 Waste items are properly discarded. Copyright © 2009, 2006 by Saunders,

Fig. 21 -3 Waste items are properly discarded. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Preparation and Packaging Area In the area set aside for preparation and packaging area,

Preparation and Packaging Area In the area set aside for preparation and packaging area, cleaned instruments and other dental supplies should be inspected, assembled into sets or trays, and wrapped or placed in packages for sterilization. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Fig. 21 -4 A modern sterilization center. Copyright © 2009, 2006 by Saunders, an

Fig. 21 -4 A modern sterilization center. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Holding Solution If a instruments cannot be cleaned immediately after a procedure, they should

Holding Solution If a instruments cannot be cleaned immediately after a procedure, they should be placed in a holding solution to prevent the drying of blood and debris on the instruments. The holding solution may be any noncorrosive liquid. A commercial enzymatic solution that partially dissolves organic debris may be used. Dishwasher detergent also makes a good holding solution because it is low-cost, low-foaming, and readily available. It is neither cost-effective nor desirable to use a disinfectant alone as a holding solution. (Cont’d) Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Holding Solution (Cont’d) The container must have a lid and must be labeled with

Holding Solution (Cont’d) The container must have a lid and must be labeled with both a biohazard label (because of the contaminated instruments) and a chemical label (because of the cleaner/detergent). The holding solution should be changed at least twice daily and even more frequently if it becomes clouded. Remember, a holding solution is necessary only when contaminated instruments cannot be processed immediately. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Fig. 21 -5 Commercial holding solutions are available for use in precleaning. (Courtesy of

Fig. 21 -5 Commercial holding solutions are available for use in precleaning. (Courtesy of Biotrol International. ) Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Precleaning and Packaging Instruments may be precleaned in one of three ways: Hand scrubbing

Precleaning and Packaging Instruments may be precleaned in one of three ways: Hand scrubbing Ø Ultrasonic cleaning Ø Instrument-washing machine Ø Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Hand Scrubbing Hand scrubbing is the least desirable method of cleaning instruments because it

Hand Scrubbing Hand scrubbing is the least desirable method of cleaning instruments because it requires direct hand contact with the contaminated instrument. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Hand-Scrubbing Instruments Wear goggle-type eyewear and puncture-resistant gloves in addition to your protective clothing.

Hand-Scrubbing Instruments Wear goggle-type eyewear and puncture-resistant gloves in addition to your protective clothing. Clean only one or two instruments at a time. Use only a long-handled brush, preferably one with a hand guard or wide surface. Keep items above the waterline; fully immersing them in a basin of soapy water interferes with your ability to see the sharp ends. Allow instruments to air-dry or carefully pat them with thick toweling. To decrease the risk of accidental injury, never rub or roll instruments in a towel. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Ultrasonic Cleaning Ultrasonic cleaners are used to loosen and remove debris from instruments. These

Ultrasonic Cleaning Ultrasonic cleaners are used to loosen and remove debris from instruments. These cleaners also reduce the risk of cuts and punctures to the hands during the cleaning process. Puncture-resistant utility gloves, a mask, protective eyewear, and a protective gown should always be worn when the ultrasonic cleaner is being used. To further limit contact with contaminated instruments, keep a set of tongs near the ultrasonic unit with which to remove instruments after the cleaning cycle. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Fig. 21 -6 Ultrasonic cleaning system. A, Ultrasonic cleaner. B, Instrument basket. C, Beaker

Fig. 21 -6 Ultrasonic cleaning system. A, Ultrasonic cleaner. B, Instrument basket. C, Beaker holder. D, Bur tray. E, Suspension bracket. F, Beaker band. G, Beaker cover. H, Glass beaker. I, Cover. (Courtesy of L & R Manufacturing Co, Kearny, NJ. ) Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Fig. 21 -7 It is important to keep the ultrasonic cleaner covered while it

Fig. 21 -7 It is important to keep the ultrasonic cleaner covered while it is in use to reduce splatter and contaminated aerosols. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Ultrasonic Cleaning The ultrasonic cleaner works by producing sound waves beyond the range of

Ultrasonic Cleaning The ultrasonic cleaner works by producing sound waves beyond the range of human hearing. These sound waves, which can travel through metal and glass containers, cause cavitation (formation of bubbles in liquid). Instruments should be processed in the ultrasonic cleaner until they are visibly clean. The time may vary from 5 to 15 minutes, depending on the amount and type of material on the instruments and the efficiency of the ultrasonic unit. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Ultrasonic Cleaning Solutions You should only use ultrasonic solutions that are specially formulated for

Ultrasonic Cleaning Solutions You should only use ultrasonic solutions that are specially formulated for use in the ultrasonic cleaner. Some ultrasonic cleaning products have enzyme activity. Other ultrasonic cleaning products have antimicrobial activity, which reduces the buildup of microbes in the solutions with repeated use. The antimicrobial activity does not disinfect the instruments; it merely prevents the microorganisms from multiplying. (Cont’d) Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Fig. 21 -8 A commercial allpurpose ultrasonic cleaner. Fig. 21 -9 An enzyme ultrasonic

Fig. 21 -8 A commercial allpurpose ultrasonic cleaner. Fig. 21 -9 An enzyme ultrasonic cleaner in tablet form. (Courtesy of Certol. ) (Courtesy of Crosstex. ) Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Ultrasonic Cleaning Solutions (Cont’d) Do not use other chemicals such as plain disinfectants in

Ultrasonic Cleaning Solutions (Cont’d) Do not use other chemicals such as plain disinfectants in the ultrasonic cleaner. Some disinfectants can “fix” the blood and debris on the instruments, making subsequent cleaning more difficult. Specific ultrasonic solutions are available for the removal of difficult materials such as cement, tartar, stains, plaster, and alginate. Refer to the instructions of the ultrasonic unit’s manufacturer regarding the specific solution to be used. The ultrasonic cleaning unit should be labeled with both a chemical label and a biohazard label because it contains a chemical and contaminated instruments. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Care of the Ultrasonic Cleaner The ultrasonic cleaning solution is highly contaminated and must

Care of the Ultrasonic Cleaner The ultrasonic cleaning solution is highly contaminated and must be discarded at least once a day or sooner if it becomes visibly cloudy. When the solution is being changed, the inside of the pan and lid should be rinsed with water, disinfected, rinsed again, and dried. All PPE should be worn while solutions are being changed in the ultrasonic cleaner. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Testing the Ultrasonic Cleaner If you notice that the instruments are not being cleaned

Testing the Ultrasonic Cleaner If you notice that the instruments are not being cleaned completely with processing in the ultrasonic cleaner, the unit may not be functioning properly. To determine whether the ultrasonic cleaner is working properly, hold a 5 -by-5 -inch sheet of lightweight aluminum foil vertically (like a curtain) half-submerged in fresh, unused solution. Run the unit for 20 seconds, then hold the foil up to the light. The surfaces that were submerged in the solution should be evenly marked with a tiny pebbling effect over the entire surface. An area without pebbling of more than ½ inch indicates a problem with the unit, and it needs to be serviced by the manufacturer. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Automated Washers/Disinfectors Automated instrument washers/disinfectors look and work similar to a household dishwasher. However,

Automated Washers/Disinfectors Automated instrument washers/disinfectors look and work similar to a household dishwasher. However, they must be approved by the U. S. Food and Drug Administration. Automated washing/disinfecting units use a combination of very hot recirculating water and detergents to remove organic material. After washing, the instruments are automatically dried. These units are classified as thermal disinfectors because they have a disinfecting cycle that subjects the instruments to a level of heat that kills most vegetative microorganisms. Instruments processed in an automatic washer/disinfector must be wrapped and sterilized before use on a patient. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Drying, Lubrication, and Corrosion Control Instruments and burs made of carbon steel will rust

Drying, Lubrication, and Corrosion Control Instruments and burs made of carbon steel will rust during steam sterilization. Rust inhibitors such as sodium nitrate and commercial products, which are available as spray and dip solution, can be used to help reduce rust and corrosion. An alternative to a rust inhibitor is to dry the instrument thoroughly with the use of dry heat or unsaturated chemical-vapor sterilization (discussed later), which does not cause rusting. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Packaging of Instruments Before sterilization, the instruments should be wrapped or packaged to protect

Packaging of Instruments Before sterilization, the instruments should be wrapped or packaged to protect them from becoming contaminated after sterilization. When instruments are sterilized without being packaged, they are exposed to the environment as soon as the sterilizer door is opened. They can be contaminated by aerosols in the air, dust, improper handling, or contact with nonsterile surfaces. An additional advantage to packaging instruments is that they can be grouped into special setups, such as crown/bridge, amalgam, prophylactic, or composite. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Fig. 21 -10 Special tartar- and stain-removing ultrasonic solution. (Courtesy of Crosstex. ) Copyright

Fig. 21 -10 Special tartar- and stain-removing ultrasonic solution. (Courtesy of Crosstex. ) Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Packaging Materials Sterilization packaging materials and cassettes are medical devices and therefore must be

Packaging Materials Sterilization packaging materials and cassettes are medical devices and therefore must be FDA-approved. It is of critical importance to use only products and materials that are labeled as “sterilization” packaging. Never substitute products such as plastic wraps, paper, or zipper-lock freezer bags that are not registered for this purpose. These products may melt or prevent the sterilizing agent from reaching the instruments inside. Specific types of packaging material are available for each method of sterilization. You should use only the type of packaging material designed for the particular method of sterilization that you are using. (Cont’d) Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Packaging Materials (Cont’d) A wide variety of sterilization packaging materials are available. Self-sealing or

Packaging Materials (Cont’d) A wide variety of sterilization packaging materials are available. Self-sealing or heat-sealed “poly” bags or tubes make excellent wraps. Paper and cloth wraps are also available. If the package is not the self-sealing type, you should only use sterilization indicator tape to seal the package. Never use safety pins, staples, paper clips, or other sharp objects that could penetrate the packaging material. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Fig. 21 -12 Self-seal packages make an excellent wrap for sterilized materials. (Courtesy of

Fig. 21 -12 Self-seal packages make an excellent wrap for sterilized materials. (Courtesy of SPS Medical Supply Corp. ) Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Sterilization of Unwrapped Instruments An unwrapped cycle (sometimes called flash sterilization) is a method

Sterilization of Unwrapped Instruments An unwrapped cycle (sometimes called flash sterilization) is a method for sterilizing unwrapped patient-care items for immediate use. The time for unwrapped sterilization cycles depends on the type of sterilizer and the type of item (i. e. , porous or nonporous) to be sterilized. Unwrapped sterilization should be used only under certain conditions. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Chapter 21 Lesson 21. 2 Copyright © 2009, 2006 by Saunders, an imprint of

Chapter 21 Lesson 21. 2 Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Learning Objectives Describe three most common methods of heat sterilization and the advantages and

Learning Objectives Describe three most common methods of heat sterilization and the advantages and disadvantages of each. Explain the primary disadvantage of flash sterilization. Explain how sterilization failures can occur. Explain the limitation of chemical liquid sterilizers. Describe three forms of sterilization monitoring. Explain the differences between process indicators and process integrators. Describe when and how biologic monitoring is done. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Methods of Sterilization destroys all microbial forms, including bacterial spores. Sterile is an absolute

Methods of Sterilization destroys all microbial forms, including bacterial spores. Sterile is an absolute term; there is no “partially sterile” or “almost sterile. ” All reusable items (critical and semicritical instruments) that come into contact with the patient’s blood, saliva, or mucous membranes must be heatsterilized. The three most common forms of heat sterilization in the dental office are Steam Ø Chemical vapor Ø Dry heat Ø Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Steam Autoclave Sterilization An autoclave is used to sterilize dental instruments and other items

Steam Autoclave Sterilization An autoclave is used to sterilize dental instruments and other items by means of steam under pressure. Steam sterilization involves heating water to generate steam, producing a moist heat that rapidly kills microorganisms. As the steam fills the sterilizing chamber, the cooler air is pushed from an escape valve, which then closes and allows the pressure to increase. It is actually the heat, not the pressure, that kills the microorganisms. (Cont’d) Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Fig. 21 -13 Steam autoclave. Copyright © 2009, 2006 by Saunders, an imprint of

Fig. 21 -13 Steam autoclave. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Steam Autoclave Sterilization (Cont’d) Packaging material for steam sterilization must be porous enough to

Steam Autoclave Sterilization (Cont’d) Packaging material for steam sterilization must be porous enough to permit the steam to penetrate to the instruments inside. The packaging material may be fabric but most often is sealed film or paper pouches, nylon tubing, sterilizing wrap, or paper-wrapped cassettes. One disadvantage of steam sterilization is that the moisture may cause corrosion on some high-carbon steel instruments. Distilled water should be used in autoclaves instead of tap water, which often contains minerals and impurities. Distilled water helps minimize corrosion and pitting. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Autoclave Operation Cycles Dental-office steam sterilizers usually operate in four cycles: Heat-up Ø Sterilization

Autoclave Operation Cycles Dental-office steam sterilizers usually operate in four cycles: Heat-up Ø Sterilization Ø Depressurization Ø Drying Ø Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Flash Sterilization Rapid or “flash” sterilization of dental instruments is accomplished by means of

Flash Sterilization Rapid or “flash” sterilization of dental instruments is accomplished by means of rapid heat transfer, steam, and unsaturated chemical vapor. Flash sterilization may be used only on instruments that are placed in the chamber unwrapped. This involves a compromise, because the sterility of the instruments is immediately defeated when the instruments are removed from the sterilizer. Flash sterilization should also be used only for instruments that are to be promptly used on removal from the sterilizer. It is always the best policy to use a method of sterilization in which the instruments can be packaged before use and remain packaged until the time of use. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Fig. 21 -15 STAT/M sterilizer. (Courtesy of Sci. Scan. ) Copyright © 2009, 2006

Fig. 21 -15 STAT/M sterilizer. (Courtesy of Sci. Scan. ) Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Chemical-Vapor Sterilization Chemical-vapor sterilization is similar to autoclaving, except that a combination of chemicals

Chemical-Vapor Sterilization Chemical-vapor sterilization is similar to autoclaving, except that a combination of chemicals (alcohol, formaldehyde, ketone, acetone, and water) is used instead of water to create a vapor for sterilization. OSHA requires a material-safety data sheet, or MSDS, on the chemical-vapor solution because of the chemicals’ toxicity. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Advantages of Chemical-Vapor Sterilization The major advantage of the chemical-vapor sterilizer is that it

Advantages of Chemical-Vapor Sterilization The major advantage of the chemical-vapor sterilizer is that it does not rust, dull, or corrode dry metal instruments. The low water content of the vapor prevents destruction of items such as endodontic files, orthodontic pliers, wires, bands, and burs. A wide range of items can be sterilized routinely without damage. Other advantages include the short cycle time and the availability of a dry instrument after the cycle. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Disadvantages of Chemical-Vapor Sterilization The primary disadvantage is that adequate ventilation is essential because

Disadvantages of Chemical-Vapor Sterilization The primary disadvantage is that adequate ventilation is essential because residual chemical vapors containing formaldehyde and methyl alcohol may be released when the chamber door is opened at the end of the cycle. These vapors can temporarily leave an unpleasant odor in the area and may be irritating to the eyes. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Filtration and Monitoring of Chemical Vapors Newer sterilizers are equipped with a special filtration

Filtration and Monitoring of Chemical Vapors Newer sterilizers are equipped with a special filtration device that further reduces the amount of chemical vapor remaining in the chamber at the end of the cycle. Older models can usually be retrofitted. Formaldehyde-monitoring badges, similar to radiation monitoring devices, are available for employees. The badge measures personal exposure to formaldehyde for a period and is then mailed to the monitoring service, which sends a laboratory analysis to the employee. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Packaging for Chemical-Vapor Sterilization Standard packaging for chemical-vapor sterilization includes film pouches or paper

Packaging for Chemical-Vapor Sterilization Standard packaging for chemical-vapor sterilization includes film pouches or paper bags, nylon seethrough tubing, sterilization wrap, and wrapped cassettes. Thick or tightly wrapped items require longer exposure because of the inability of the unsaturated chemical vapors to penetrate as well as saturated chemical vapors under pressure do. As with autoclaving, closed containers (e. g. , solidmetal trays and capped glass vials) and aluminum foil cannot be used in a chemical-vapor sterilizer because they prevent the sterilizing agent from reaching the instruments inside. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Pressure, Temperature, and Duration of Chemical-Vapor Sterilization The three major factors in chemical-vapor sterilization

Pressure, Temperature, and Duration of Chemical-Vapor Sterilization The three major factors in chemical-vapor sterilization are: Pressure, which should measure 20 psi Ø Temperature, which should measure 131° C (270° F) Ø Time, which should measure 20 to 40 minutes Ø Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Dry-Heat Sterilization Dry-heat sterilizers operate by heating up air and transferring that heat from

Dry-Heat Sterilization Dry-heat sterilizers operate by heating up air and transferring that heat from the air to the instruments. This form of sterilization requires higher temperatures than does steam or chemical-vapor sterilization. Dry-heat sterilizers operate at approximately 160° to 190° C (320°-375° F), depending on the type of sterilizer. The advantage of dry heat is that the instruments will not rust if they are thoroughly dry before being placed in the sterilizer. There are two types of dry-heat sterilizers: static-air and forced-air. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Static-Air Sterilizers The static-air sterilizer is similar to an oven: The heating coils are

Static-Air Sterilizers The static-air sterilizer is similar to an oven: The heating coils are on the bottom of the chamber, and the hot air rises inside by way of natural convection. Heat is transferred from the static (nonmoving) air to the instruments in 1 to 2 hours. Disadvantages of static dry heat: The sterilization process is time-consuming, and it may not be effective if the operator errs in calculating the correct processing time. The wrapping material must be heat-resistant. Aluminum foil, metal, and glass containers may be used. Paper and cloth packs should be avoided because they may burn or discolor in the intense heat. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Forced-Air Sterilizers The forced-air sterilizer, also called the rapidheat-transfer sterilizer, circulates the hot air

Forced-Air Sterilizers The forced-air sterilizer, also called the rapidheat-transfer sterilizer, circulates the hot air throughout the chamber at a high velocity. This action permits rapid transfer of heat energy from the air to the instruments, reducing the time needed for sterilization. Exposure time in a forced-air sterilizer, after the sterilizing temperature has been reached, ranges from 6 minutes for unpackaged items to 12 minutes for packaged items. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Ethylene Oxide Sterilization The use of ethylene oxide gas is a recognized method of

Ethylene Oxide Sterilization The use of ethylene oxide gas is a recognized method of sterilization. This method is carried out at low temperatures, which is an advantage for plastic and rubber items that would melt in heat sterilizers. However, ethylene oxide sterilization requires 4 to 12 hours, depending on the sterilizer model, and at least 16 hours of poststerilization aeration is required to remove the gas molecules bound to plastic and rubber surfaces. Ethylene oxide is ineffective on wet items. Toxicity is possible if the gas is not handled properly. Ethylene oxide sterilizing units are often used in large clinics or hospital settings but are only rarely found in private dental practices. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Chemical-Liquid Sterilization Not all items can withstand heat sterilization. Some types of plastics, such

Chemical-Liquid Sterilization Not all items can withstand heat sterilization. Some types of plastics, such as some rubber dam frames, shade guides, and x-ray film–holding devices, are damaged by heat sterilization. A liquid sterilant such as 2. 0% to 3. 4% glutaraldehyde must be used for sterilization of these items. Sterilization in glutaraldehyde requires 10 hours of contact time; anything less than 10 hours is disinfection, not sterilization. Be sure that you have an MSDS for these products. All employees should be properly trained to handle them. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Fig. 21 -17 SPOROX II is a high-level disinfectant/sterilant used for instruments that cannot

Fig. 21 -17 SPOROX II is a high-level disinfectant/sterilant used for instruments that cannot tolerate heat sterilization. (Courtesy of Sultan Chemists Inc, Englewood, NJ. ) Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Sterilization Failures Sterilization may fail when direct contact for the correct time between the

Sterilization Failures Sterilization may fail when direct contact for the correct time between the sterilizing agent (chemical or steam) and all surfaces of the items being processed is insufficient. Several factors can cause the sterilization process to fail, including improper instrument cleaning or packaging and sterilizer malfunction. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Table 21 -7 Results of Sterilization Errors. Copyright © 2009, 2006 by Saunders, an

Table 21 -7 Results of Sterilization Errors. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Sterilization Monitoring It is critical that dental instruments be properly sterilized. Ø Because microorganisms

Sterilization Monitoring It is critical that dental instruments be properly sterilized. Ø Because microorganisms cannot be seen with the naked eye, the major difficulty in sterilization is determining when an item is sterile. Currently, three forms of sterilization monitoring are used: physical, chemical, and biologic. Ø All three processes are unique, have different functions, and must be used consistently to ensure sterility. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Physical Monitoring Physical monitoring of the sterilization process involves looking at the gauges and

Physical Monitoring Physical monitoring of the sterilization process involves looking at the gauges and readings on the sterilizer and recording temperatures, pressure, and exposure time. Although correct readings do not guarantee sterilization, an incorrect reading is the first signal of a problem. Remember that the reading reflects the temperature in the chamber, not inside the pack. For this reason, problems with overloading or improper packaging would not be revealed by the reading on the gauges. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Chemical Monitoring Chemical monitoring (external and internal) involves the use of a heat-sensitive chemical

Chemical Monitoring Chemical monitoring (external and internal) involves the use of a heat-sensitive chemical that changes color when exposed to certain conditions. There are two types of chemical indicators: Process indicators Ø Process integrators Ø Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Process Indicators Process indicators (external) are placed outside the instrument packages before sterilization. Examples

Process Indicators Process indicators (external) are placed outside the instrument packages before sterilization. Examples include autoclave tape and color-change markings on packages or bags. Process indicators simply identify instrument packs that have been exposed to a certain temperature; they do not indicate duration or pressure. Process indicators are useful in distinguishing between packages that have been processed and those have not. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Fig. 21 -18 A, Unprocessed instruments. B, Wrapped instruments after processing. Note the color

Fig. 21 -18 A, Unprocessed instruments. B, Wrapped instruments after processing. Note the color change in the tape. (From Young AP, Kennedy DB: Kinn’s the medical assistant: an applied learning approach, ed 9, Philadelphia, 2003, Saunders. ) A Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved. B

Process Integrators Process integrators (internal) are placed inside instrument packages. They respond to a

Process Integrators Process integrators (internal) are placed inside instrument packages. They respond to a combination of pressure, temperature, and time. Process integrators are also known as multiparameter indicators. All sterilization factors are integrated. Process indicators and integrators provide immediate visual control of sterilizing conditions. They do not indicate sterility and are not a replacement for biologic monitoring. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Biologic Monitoring Biologic monitoring, or spore testing, is the only way to determine whether

Biologic Monitoring Biologic monitoring, or spore testing, is the only way to determine whether sterilization has occurred and all bacteria and endospores have been killed. The CDC, American Dental Association, and Office of Safety and Asepsis Procedures Research Foundation recommend at least weekly biologic testing of sterilization equipment. Several states also require routine biologic checks at weekly, monthly, or cycle-specific intervals, such as spore testing every 40 hours of use or every 30 days. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Biologic Indicators Also known as spore tests, biologic indicators (BIs) are vials or strips

Biologic Indicators Also known as spore tests, biologic indicators (BIs) are vials or strips of paper that contain harmless bacterial spores (which are highly resistant to heat). Three BIs are used in testing. Two BIs are placed inside instrument packs, and the sterilizer is operated under normal conditions. The third strip is set aside as a control. After the load has been sterilized, all BIs are cultured. If the spores survive the sterilization cycle (a positive culture), sterilization failure has occurred. If the spores are killed (a negative culture), the sterilization cycle was successful. The culturing of the spore test is usually handled with the use of a mail-in monitoring service. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Fig. 21 -21 Use of a mail-in service is a convenient method of biologic

Fig. 21 -21 Use of a mail-in service is a convenient method of biologic monitoring. Fig. 21 -22 In-office biologic monitoring system. (Courtesy of Certol. ) (Courtesy of SPSmedical Supply Corp. ) Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Chapter 21 Lesson 21. 3 Copyright © 2009, 2006 by Saunders, an imprint of

Chapter 21 Lesson 21. 3 Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Learning Objectives Describe the steps in cleaning and sterilization of the high-speed dental handpiece.

Learning Objectives Describe the steps in cleaning and sterilization of the high-speed dental handpiece. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Handpiece Sterilization High-speed dental handpieces rotate at speeds up to 400, 000 revolutions per

Handpiece Sterilization High-speed dental handpieces rotate at speeds up to 400, 000 revolutions per minute (rpm). Blood, saliva, and tooth fragments, as well as restorative materials, may lodge in the head of the handpiece, where they may be retained and transferred to another patient. Therefore, dental handpieces must be properly cleaned and heat-sterilized. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Handpiece Flushing Techniques Flushing the handpiece is the best way to remove debris from

Handpiece Flushing Techniques Flushing the handpiece is the best way to remove debris from the head of the handpiece. To flush a dental handpiece: Attach a pressurized handpiece cleaner to the intake tube of the handpiece (where the air passes through). Ø Flush the head of the handpiece to remove debris. Ø Blow out the handpiece using compressed air to remove debris before sterilization. Ø Running coolant water from the dental unit through the handpiece at chairside is insufficient. Coolant water does not run through the turbine chamber, where debris can collect. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.

Handpiece Sterilization Techniques Only steam sterilization and chemical vapor sterilizers are recommended, because handpiece

Handpiece Sterilization Techniques Only steam sterilization and chemical vapor sterilizers are recommended, because handpiece sterilization temperatures should not exceed 275° F (135° C). Unless they will be used immediately after sterilization, handpieces should be packaged in bags, wraps, or packs to protect them from contamination before use. Never run a handpiece “hot” out of the sterilizer, and avoid rapid cool-downs, such as running the handpiece under cold water. Handpieces use very small metal components; extreme cold changes stresses the metal. If handpieces need to be cooled quickly after sterilization, use an air fan to blow room-temperature air over them. Copyright © 2009, 2006 by Saunders, an imprint of Elsevier Inc. All rights reserved.