Dr James Watson codiscoverer of the DNA helix

День Генома Dr. James Watson, co-discoverer of the DNA helix and father of the Human Genome Project, became the first human to receive the data encompassing his personal genome sequence at Baylor College of Medicine in Houston in May 31, 2007. James Watson (left) receives a digital copy of his genome sequence from Jonathan Rothberg in May 2007.

Next Generation Sequencing (NGS) 1. Pyrosequencing 2. Cluster-SBS 3. SOLi. D

Jonathan Rothberg – основатель компании 454 Life Sciences Dr. Rothberg was born in 1963 in New Haven, M. S. , M. Phil, and Ph. D. in biology from Yale University. Dr. Rothberg is the founder of 454 Life Sciences, Clarifi Corporation, Cura. Gen Corporation, The Rothberg Institute for Childhood Diseases, and the co-founder and Chairman of Rain. Dance Technologies.

Распределение длины FLX-сиквенсов (Titanium) Run completed with improved Genome Sequencer FLX Titanium series chemistry demonstrating a modal read length of 766 base pairs. http: //www. 454. com/products-solutions/future-of-454 -sequencing. asp

Парноконцевые библиотеки ДНК http: //www. 454. com/downloads/De. Novo. Complex. Genomes_Flyer. pdf 23 Apr 2009

Области применения • Metagenomic Analysis • RNA Virus Sequencing • Small RNA Analysis • Comprehensive Analysis of Micro. RNAs • Transcriptome sequencing • Expression tags • Ch. IP-seq/Methylation/Epigenetics • Amplicon Sequencing • Mitochondria/Viruses/Plastids/Plasmids • Ancient DNA • Prokaryote Whole Genome Eukaryote Whole Genome ?

SBS / SBL (sequencing by synthesis / sequencing by ligation) Полимеразный синтез ДНК Лигазный синтез ДНК A new class of cleavable fluorescent nucleotides: synthesis and optimization as reversible terminators for DNA sequencing by synthesis / Turcatti G. , Romieu A. , Fedurco M. , Tairi A. -P. // Nucleic Acids Research, 2008, Vol. 36, No. 4 e 25. 5’ 3’ 3’ 5’

Динамика роста производительности cеквенаторов SOLi. D http: //www 3. appliedbiosystems. com/AB_Home/applicationstechnologies/SOLi. DSystem. Sequencing/overviewofsolidsystem/index. htm

Mate-Paired & Paired End Reads

SMRT sequencing (Single-Molecule, Real-Time sequencing)

True Single Molecule Sequencing (t. SMS) • Two glass flow cells • Each flow cell is divided into 25 channels • 50 individual samples per run • Proprietary surface chemistry • ~25 bases in length • Multi-pass sequencing Heli. Scope™

Polonator G. 007

Прочие конкуренты Ultra-Fast Real Time sequencer Single Molecule Sequencing Gene. Engine™ technology Visi. Gen® sequencing system GE Global Research

Nanopore Sequencing

http: //genomics. ucsd. edu/yingja/

The Beijing Genomics Institute (BGI) http: //www. genomics. org. cn/en/platform_detail. php? id=150