- Slides: 29
DNA, Chromosomes and the Nucleus
DNA Structure § What are some of the important structural features of the DNA double helix?
DNA Structure § In addition to B-DNA what other forms exist?
DNA Structure § What is supercoiled DNA and what effect does it have? § Can it occur in linear DNA molecules?
DNA Structure § What is the difference between topoisomerases and DNA gyrase? § How can one bring about the denaturation and renaturation of DNA?
DNA Structure § What is the DNA melting temperature?
Genomic DNA § How do we define the term “genome”? § How is genomic size expressed? § Is there a relationship between genomic size and complexity of the organism or virus?
Genomic DNA § What are restriction enzymes and how have they helped us to study DNA?
Genomic DNA § How can restriction fragments be characterized?
Genomic DNA § How is restriction mapping carried out and what information does it provide?
Genomic DNA § How is the chain termination method of Sanger used to determine the sequence of a DNA molecule?
Genomic DNA § What is bioinformatics and proteomics? § How much variation is there in the human genome?
Genomic DNA § How did the work of Britten and Kohne help explain why eukaryotic genomes are so large?
Genomic DNA § What is the difference between tandemly repeated DNA versus interspersed repeated DNA?
Genomic DNA § What are the possible functions of this tandemly repeated DNA? § What about the role of this DNA fingerprinting? § in certain genetic diseases?
DNA Packaging § How is the bacterial genome structured to fit inside of the small cell? § What are bacterial plasmids?
DNA Packaging § What is the relationship between DNA and chromosomes in eukaryotic cells? § What are nucleosomes?
DNA Packaging § What experimental evidence supported the existence of nucleosomes?
DNA Packaging § How is the nucleosome organized? Where is the H 1 histone protein?
DNA Packaging § How are nucleosomes packaged to form chromosomes? § What is the DNA packing ratio?
DNA Packaging § How does mitocondrial and chloroplast DNA compare with nuclear DNA?
Nucleus § What do we know about nuclear structure?
Nucleus § What are the major features of the nuclear pore complex?
Nucleus § What kinds of molecules are transported across the nuclear envelope?
Nucleus § How are molecules transported across the nuclear envelope?
Nucleus § What role does the nuclear matrix serve? § What role does the nuclear lamina serve? § What is the function of the nucleolus?