Pentose Phosphate CH 339 K Pentose Phosphate An
- Slides: 33
Pentose Phosphate CH 339 K
Pentose Phosphate An example of a pathway that can be both: • Anabolic – – • Generates ribose-5 -phosphate for nucleotide synthesis Metabolizes dietary pentoses into glycolytic/gluconeogenic intermediates. Catabolic – – – Generates reducing power (NADPH) Can completely oxidize glucose Can carry on into glycolysis Aka Hexose Monophosphate Shunt
NADH vs NADPH As a general rule of thumb: • NAD+/NADH is used in catabolic processes • NADP+/NADPH is used in anabolic processes
G 3 P to Glycolysis
Oxidative Phase 1) Lose a carbon 2) Reduce 2 NADP+ lactonase H 2 O
Better Picture
Glutathione -SH containing tripeptide Glu-Cys-Gly Amino of Cysteine linked to g-carboxyl of glutamate Commonly used for reducing agent in cells Oxidizes to for disulfide-linked GSSG Rereduced to GSH using NADPH
2 GSH NADP+ Glutathione Reductase NADPH + H+ GSSG
Making Glutathione • For the Reaction to form GSH: GSSG + 2 e- + 2 H+ ⇄ 2 GSH -0. 23 V NADPH + H+ ⇄ NADP+ + 2 e- + 2 H+ +0. 32 V GSSG + NADPH + H+ ⇄ 2 GSH + NADP+ +0. 09 V We can figure out DGo from what we learned about redox reactions
For what it’s worth… Divicine is found in fava beans and some other legumes Favas (broad beans) are common foodstuffs in the old world. Largest production in Europe and China. The parent plant, Vicia faba, is among the oldest cultivated plants ~6, 000 years.
Glucose-6 -P Dehydrogenase Deficiency • • • Effects ~ 4*108 people worldwide Most common human genetic disease Lack of G-6 PD means lack of NADPH Lack of NADPH means lack of GSH Lack of GSH means excess of peroxides RBC membranes particularly susceptible to peroxides • Hemolytic Anemia
Harmful Agents for G 6 PDD Sufferers Antimalarials Analgesics Antibiotics Anthelmintics Miscellaneous Primaquine Pamaquine Chloroquine Aspirin Bufferin Anacin Excedrin Empirin APC Tablets Darvon Compound Coricidin Sulfanilamide Sulfapyridine Sulfadimidine Sulfacetamide Glucosulfone sodium Nitrofurantoin Furazolidone Nitrofurazone Dapsone Sulfoxone Sulfisoxazole B-Naphthol Stibophen Niridazole Probenecid Thiazide Diuretics Phenothiazine Chloramphenicol Orinase Dimercaprol Methylene blue Naphthalene (moth balls) Vitamin K Fava beans
G 3 P to Glycolysis
Non-oxidative phase
Transketolase moves 2 -carbon units
Transaldolase moves 3 -carbon units Lack of transketolase can cause hepatosplenomegaly and liver cirrhosis in childhood. Verhoeven, N. M. et al (2001) Transaldolase Deficiency: Liver Cirrhosis Associated with a New Inborn Error in the Pentose Phosphate Pathway , Amer. J. Hum. Gen. 68(5): 1086 -1092.
Control • Conversion of glucose-6 -Pi to the lactone is essentially irreversible. • The enzyme, glucose-6 -phosphate dehydrogenase, controls the rate of the pathway. – NADPH competes with NADP for binding in he active site; – ATP competes with glucose-6 -phosphate. • At high [NADPH] and/or high [ATP], entrance into the pathway is restricted.
Products in the pathway can be withdrawn at several points
- Pentose phosphate shunt
- Pentose phosphate pathway
- Glucogenesis vs gluconeogenesis
- 6 phosphogluconate structure
- Pentose phosphate pathway
- Purpose of pentose phosphate pathway
- Oxidative pentose phosphate pathway
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- Lesson 8 extra practice quadratic functions
- Pentose sugar structure in dna
- Monosaccharide
- Where are polysaccharides found
- Barfoed's test for carbohydrates
- Lewis dot shapes
- Calcium phosphate atomic mass
- Bone fluid
- Khbo2
- Magnesium ammonium phosphate stones
- Frameshift
- Iron(iii) chloride (aq) + cesium phosphate (aq)
- What is a phosphate head
- The body's three energy systems
- Deamination of glutamine
- Glucogenic amino acids
- Phosphate buffer system equation
- Enzyme function in metabolism
- Phosphate formula and charge
- Zinc phosphate cement composition
- Fates of glucose 6 phosphate
- The phosphorus cycle
- Cell membrane phosphate head
- Phosphate binders examples
- H2po4
- Triphosphorus pentasulfide