Pro Star 325 Detector Petro van Poppel Field

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Pro. Star 325 Detector Petro van Poppel – Field Support Specialist Friday, 2 October

Pro. Star 325 Detector Petro van Poppel – Field Support Specialist Friday, 2 October 2020

Overview Introduction to PS 325 Detector • Dual Wavelength Option • LC Verify Software

Overview Introduction to PS 325 Detector • Dual Wavelength Option • LC Verify Software • Flow cells • Communications LC Verify • Production Tests with 0 x 0 flow cell • Wavescan Test (Mono Options) • Diagnostics Mode (Main Tab) • Electronics Test (Input/Output) • System Details (EEPROM) • Communications (COMM) PS 335 Shipping Test Problems

325 Optics Module Light Path Vis D 2

325 Optics Module Light Path Vis D 2

325 Optics Ray Beam

325 Optics Ray Beam

Dual Wavelength Option Dual wavelength is a firmware change that allows you to view

Dual Wavelength Option Dual wavelength is a firmware change that allows you to view 2 wavelengths “simultaneously” Galaxie Software LC Diagnostics Software

325/335 Back Panel P/N 02 -101875 -00 Relay Adaptor board I/O adaptor board for

325/335 Back Panel P/N 02 -101875 -00 Relay Adaptor board I/O adaptor board for the sync signals P/N 02 -101865 -00

Flow Cell Ray Beam General design of the 9 x 0 flow cell General

Flow Cell Ray Beam General design of the 9 x 0 flow cell General design of the 9 x 1 flow cell

Flow Cell Specs Flow Cell Prep (9 x 1) Pressure rating 1000 psi Flow

Flow Cell Specs Flow Cell Prep (9 x 1) Pressure rating 1000 psi Flow Cell Volume 30 µl Material (non Ti & PEEK optical) Noise Performance Super Prep (4 x 0. 15) Analytical (9 x 0) Micro (4 x 0 ) 1000 psi 145 µl 1. 5 µl Ti & PEEK 200 ml/min 10 -500 ml 800 ml/min 0. 80 -10 L 40 ml/min 0. 1 µl-100 ml 1 ml/min 0 -20 ml 1 x 10 -4 AU 5 x 10 -3 AU 7. 5 x 10 -3 AU

Communications TCP/IP communications for Detector: • Static or Dynamic • Networked or standalone •

Communications TCP/IP communications for Detector: • Static or Dynamic • Networked or standalone • Firewall • Access on Network, OS or PC

LC Verify side

LC Verify side

LC Diagnostics Side Ctrl-Shift-F 10 to enable Diagnostics side Ctrl-Shift-F 9 to hide Diagnostics

LC Diagnostics Side Ctrl-Shift-F 10 to enable Diagnostics side Ctrl-Shift-F 9 to hide Diagnostics side

PS 325 Test Certificate provided by factory Parameters tested: 1) Noise 2) Wavelength Accuracy

PS 325 Test Certificate provided by factory Parameters tested: 1) Noise 2) Wavelength Accuracy 3) Wavelength Reproducibility 4) Spectral Bandwidth Accuracy 5) Auto. Zero Precision Test 6) Detector Linearity Test

LC Verify use in Final Testing

LC Verify use in Final Testing

PS 325 Noise Test (FT)

PS 325 Noise Test (FT)

PS 325 Wavelength Accuracy Test (FT)

PS 325 Wavelength Accuracy Test (FT)

PS 325 Wavelength Repeatability Test (FT)

PS 325 Wavelength Repeatability Test (FT)

PS 325 Spectral Bandwidth Test (FT)

PS 325 Spectral Bandwidth Test (FT)

PS 325 Auto Precision Test (FT)

PS 325 Auto Precision Test (FT)

PS 325 Detector Linearity Test (FT) pump water for at least 10 minutes at

PS 325 Detector Linearity Test (FT) pump water for at least 10 minutes at a pump head pressure of at least 100 atm using a back pressure restrictor of 40 psi.

PS 325 LCVerify Wavescan

PS 325 LCVerify Wavescan

PS 325 Wavescan results

PS 325 Wavescan results

PS 325 Contributions from each Lamp

PS 325 Contributions from each Lamp

PS 325 LCVerify Diagnostics Mode

PS 325 LCVerify Diagnostics Mode

PS 325 LCVerify Input/Output Test

PS 325 LCVerify Input/Output Test

PS 325 Main PWB Power Supply LED’s A: + 24 V power Supply B:

PS 325 Main PWB Power Supply LED’s A: + 24 V power Supply B: + 9 V and – 9 V Power Supply C: + 5 V Power Supply C A Front of the Unit B

PS 325 LCVerify System Details

PS 325 LCVerify System Details

PS 325 LCVerify Communications

PS 325 LCVerify Communications

Caffeine Shipping Test PS 325 Caffeine Shipping Test: Test made up of 1 components:

Caffeine Shipping Test PS 325 Caffeine Shipping Test: Test made up of 1 components: • Loop injection using 25 µg/ml caffeine Test is conducted using Galaxie Software

PS 325 Lamp Intensity Test 1: Lamp Intensities

PS 325 Lamp Intensity Test 1: Lamp Intensities

PS 325/335 Caffeine Loop Injection Test Calculates: Peak Height Peak Area Noise S/N

PS 325/335 Caffeine Loop Injection Test Calculates: Peak Height Peak Area Noise S/N

Common Problems • High Noise • Lamp on, not ready • SIG_CC_ZERO_ORDER_PEAK_FIND_FAILED • SIG_CC_SIGNAL_OVERRANGE_REPREFORM_

Common Problems • High Noise • Lamp on, not ready • SIG_CC_ZERO_ORDER_PEAK_FIND_FAILED • SIG_CC_SIGNAL_OVERRANGE_REPREFORM_ LAMP_CAL • COM_CC_CIRCULAR_BUFFER_OVERRUN _OCCURED • 9555 LMP_CC_D 2_FILAMENT_FAILURE • PROSTAR 325 DRIVER: Collection not started - Acquisition time too short for the method. • Foggy Optics PS 325 Error Messages Table can be found: • PS 325 Service Manual • Dealernet

Foggy Optics What is Foggy Optics?

Foggy Optics What is Foggy Optics?

What is Foggy Optics? Three types of Foggy optics • White powdery coating •

What is Foggy Optics? Three types of Foggy optics • White powdery coating • Rust/brown spot burn • Speckled mirror (delamination)

Foggy Optics What is Foggy Optics? Diagnosing Foggy Optics

Foggy Optics What is Foggy Optics? Diagnosing Foggy Optics

Diagnosing Foggy Optics Run a “wavescan” test, When bad Open the optics and inspect.

Diagnosing Foggy Optics Run a “wavescan” test, When bad Open the optics and inspect.

Wavescan test results Intensity for the 254 nm line Currently is 10 – 50

Wavescan test results Intensity for the 254 nm line Currently is 10 – 50 K counts

False positive New D 2 vs Aged D 2 Intensities

False positive New D 2 vs Aged D 2 Intensities

Foggy Optics What is Foggy Optics? Diagnosing Foggy Optics Treating Foggy optics

Foggy Optics What is Foggy Optics? Diagnosing Foggy Optics Treating Foggy optics

0603 Project (Foggy Optics) Proposed Fixes and their status: White powder fogging: due to

0603 Project (Foggy Optics) Proposed Fixes and their status: White powder fogging: due to leaky flow cells and customer sample depositing on the mirrors New Flow cells & fittings available for all cells except 4 x 0. 15 Brown powder fogging: due to UV attack on some of the foam parts close to the UV source. Eliminating as much of the foam as possible and shielding the rest. Mirror delamination: due to UV attack on the thin Al coating of the mirrors Eliminated by putting a Ni. Cr coating on the mirrors to strengthen the Al coating.

Opening and cleaning the optics

Opening and cleaning the optics

Cleaning 325 optics Clean area in circle Do not adjust!!

Cleaning 325 optics Clean area in circle Do not adjust!!

Replacing Optics Wavelength calibration data must be entered into the 335 EEPROM

Replacing Optics Wavelength calibration data must be entered into the 335 EEPROM

Summary • Single and Dual Wavelength, UV and UV/vis • Optical path is open

Summary • Single and Dual Wavelength, UV and UV/vis • Optical path is open • Flow cell is dual path • Diagnostics Tests, like wavescan • Shipping Test • Main Problems (Foggy Optics)