Platelet adhesion aggregation test Platelet Multifunction test 40

  • Slides: 24
Download presentation

공식학술명 : 혈소판 부착 및 응집능검사 (Platelet adhesion & aggregation test) 보험분류 : 혈소판

공식학술명 : 혈소판 부착 및 응집능검사 (Platelet adhesion & aggregation test) 보험분류 : 혈소판 복합 기능 검사 (Platelet Multi-function test) 보험수가 (비급여): 40, 000원 원리 : High shear test system 검사결과 : CT (Closure Time) 검사시간 : <5분

Platelet Function Test Conventional tests Bleeding time Platelet aggregation test New tests PFA-100 Verify.

Platelet Function Test Conventional tests Bleeding time Platelet aggregation test New tests PFA-100 Verify. Now

® PFA-100 system Built-In Printer LCD screen Soft keys Test cartridge Trigger solution Cassette

® PFA-100 system Built-In Printer LCD screen Soft keys Test cartridge Trigger solution Cassette Carousel

Two kinds of Test Cartridges Collagen/Epinephrine Routine screening High sensitivity Detects Aspirin (like) effects,

Two kinds of Test Cartridges Collagen/Epinephrine Routine screening High sensitivity Detects Aspirin (like) effects, v. WD Thrombocytopathies, etc. Collagen/ADP ASA confirmation Detection of v. WD, Thrombocytopathies, etc.

Test principle (High shear test system) In Vivo Hemostasis Endothelium Collagen PFA-100® Aperture 150

Test principle (High shear test system) In Vivo Hemostasis Endothelium Collagen PFA-100® Aperture 150 µm Collagen+ EPI or ADP v. WF Fibrinogen Platelet Erythrocyte 200µm - 40 mbar Erythrocyte

operation Steps Easy Three Start test Insert test cartridge Pipette 800µl sample

operation Steps Easy Three Start test Insert test cartridge Pipette 800µl sample

15 sec 45 sec 80 sec 110 sec

15 sec 45 sec 80 sec 110 sec

Closure Time (CT) in SECONDS indicating “Platelet Haemostasis Capacity (PHC)” PFA-100 REV. 2. 20

Closure Time (CT) in SECONDS indicating “Platelet Haemostasis Capacity (PHC)” PFA-100 REV. 2. 20 S/N: 2179 20/02/04 ID#: 09: 50 394179 Test Type: Collagen/EPI SAMPLE A: 110 SEC

Interpretation 참고치 범위 (3. 2% sodium citrate tube) Collagen / EPI : 81 -192

Interpretation 참고치 범위 (3. 2% sodium citrate tube) Collagen / EPI : 81 -192 sec (국내 n=120) , 5% 범위 내 (본원 n=28) Collagen / ADP : 61 -110 sec (국내 n=120) , 5% 범위 내 (본원 n=25) 결과 해석 Normal Aspirin v. WD Other platelet disorder Col/EPI Normal Abnormal Col/ADP Normal Abnormal

Positive Bleeding History n=628 (11. 2%) Abnormal Screening Tests n=256 (4. 5%) PFA-100® (COL/EPI)

Positive Bleeding History n=628 (11. 2%) Abnormal Screening Tests n=256 (4. 5%) PFA-100® (COL/EPI) BT APTT PT Protein C All Patients n=5649 n=250 (98%) n=188 (73%) n=24 (9%) n=10 (4%) n=2 (1%)

PFA-100® (COL/EPI) n=250 (98%) BT n=188 (73%) APTT n=24 (9%) PT n=10 (4%) PC

PFA-100® (COL/EPI) n=250 (98%) BT n=188 (73%) APTT n=24 (9%) PT n=10 (4%) PC n=2 (1%) Secondary haemostatic Disorders = 2명 Primary haemostatic disorders; 187명 Combined haemostatic - Drug induced 162명 disorders; 67명 (Aspirin 87명) - Vwd 54명 PFA-100은 preoperative bleeding risk원인의 97. 8%에 해당하는 1차 지혈이상과 Vwd 를 screen

Collagen / ADP CT : Normal CT : Abnormal 5 1 87 27 Collagen

Collagen / ADP CT : Normal CT : Abnormal 5 1 87 27 Collagen / Epinephrine CT : Normal Collagen / Epinephrine CT : Abnormal 120 samples, Aspirin ingestion (325 mg) Platelet dysfunction detected in Aspirin indicated in 95. 0 % 72. 5 %

PFA-100 영향 인자 검체 내의 microthrombin 혈액형 Hematocrit level (<30%) 혈소판 수 (<50, 000

PFA-100 영향 인자 검체 내의 microthrombin 혈액형 Hematocrit level (<30%) 혈소판 수 (<50, 000 ~ 80, 000/ul) Lipemic sample 용혈

Test numbers of bleeding time

Test numbers of bleeding time

Test numbers of Col/Epi

Test numbers of Col/Epi

Test numbers of Col/ADP

Test numbers of Col/ADP