The Pedigree Method History Practice of the pedigree
- Slides: 22
The Pedigree Method
History • Practice of the pedigree method predates rediscovery of Mendel’s work • Vilmorin used the pedigree method in France in the 1830’s • Institute at Svalof - used pedigree type breeding in the 1880’s
Nillson • Represented families by one or more plants • Noted that when harvested seed came from only one plant, the progeny were much more uniform • Came to call this the “system of pedigree”
Begin with the F 2 generation • May be space planted to provide maximum individual plant expression • May be planted at the same density as the crop normally is
F 2 plant selection • Identify best plants, e. g. short, early, clean, vigorous • Tag or spray paint plants to signify selects • May tag at flowering to indicate traits that will not be readable at maturity • Historical - pull or dig up whole plants at maturity to provide plenty of F 2: 3 seed
F 2 Selection • To harvest (dig up) single plants or simply harvest single heads is a decision with several implications • Single head harvest is much quicker and easier, but it limits the amount of seed and thus the experimental unit that is grown in the F 3
F 2 selection • Space-planted or solid seeded - another critical decision • Space planted populations allow for individual plant expression, maximum tillering (etc. ), and maximum individual plant yield • But if it does not mirror the way the crop is grown, how “real” is it?
Variability Issues Generation Among Within F 2: 3 1 0. 5 F 3: 4 F 4: 5 1. 75 0. 25 0. 125 F 5: 6 1. 875 0. 0625
Selection Pressure / No. of Lines • Maximize the number of F 2: 3 lines because the among line variation is greater than the within line variation • If you do not maximize the number of distinct F 2: 3 families, then you will be relying on within line variation, which is of diminishing magnitude as inbreeding progresses.
Selection Pressure / No. of Lines • If you have selected only 5 - 10 plants from an F 2 population, then you will be relying on variation within inbred lines that trace back to those few F 2 plants. • The problem is that theory tells us that the within line variation will simply not be there.
Pedigree Information • The keeping of pedigree information is very tedious • One rationale for keeping it is to make sure that lines trace back to different F 2 plants
Pedigree Information • Sister lines - what are they? • Pedigree information allows us to keep track of sister lines • E. g. 1) KY 95 C - 100 - 14 -23 - 8 - 6 - 0 and 2) KY 95 C - 100 - 14 -25 -2 - 0 and 3)KY 95 C - 100 - 14 -23 - 8 - 12 - 0 • 1 and 2 are sisters at the F 2: 3 level and 1 and 3 are sisters at the F 4: 5 level
Characters for Selection • High heritability desirable • Amenable to visual selection • Traits that can be evaluated in individual plants or progeny rows • Number of plants grown must be adequate for number of traits evaluated • Must maintain sufficient number of plants such that variability for low h 2 traits like yield still remains.
Choice of Environments • Assume F 2 will be grown in main nursery environment (ex. Lexington) • With pedigree selection, early generations will all be grown there because individual plant selection is practiced • Alternative is to grow a bulk version of a generation at another location
Pros • Discard inferior types early • Each generation grown in different year which exposes material to different environments • Genetic relatedness of lines known, so variability among lines retained can be maximized
Cons • Can’t be used in environments where genetic variability for trait not expressed (off season nursery) • Tremendous record keeping • May need experienced person to select • Land labor intensive
Pedigree Selection • Most breeders use some aspect of it • Few breeders use very strict pedigree selection • Too expensive and labor intensive • May be most suitable for small program where quality considerations limit size and genetic variability (e. g. , malting barley)
Pedigree of Compton Wheat Fulhio / Purkof /3/ Trumbull // Hope / Hussar /5/ Fairfield /4/ PI 94587 /3/ Hungarian // Fultz / Hungarian /8/ La. Porte /7/ Minhardi / Wabash /5/ Fultz / Hungarian // Illinois No. 1, W 38 /3/ Wabash /4/ Fairfield /6/ Redcoat / Wisconsin 245 / Afghanistan /9/4* Knox /8/( Purdue 5127 ) Kawvale /5/ Fultz / Hungarian // Illinois No. 1, W 38 /3/ Wabash /4/ Fairfield /6/3* Trumbull // Hope / Hussar /7/ Knox sib /5/ Fairfield /4/ PI 94587 // Fultz / Hungarian /3/ Fultz / Hungarian / 10 /2* Knox / Frex // Riley sib.
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