Peshawar Medical College NWFP Pakistan Peshawar Medical College

Peshawar Medical College NWFP Pakistan

Peshawar Medical College NWFP Pakistan

Phosphorus Peshawar Medical College NWFP Pakistan

Phosphorous P At. No. 15 Atomic Mass: 30. 77 Peshawar Medical College NWFP Pakistan

PHOSPHORUS Peshawar Medical College NWFP Pakistan

Learning Objectives • Dietary sources • Daily Requirements • Metabolism • Important functions and • Related health problems Peshawar Medical College NWFP Pakistan

• Inorganic phosphate (Pi) is required for cellular function and skeletal mineralization. • Serum Pi level is maintained within a narrow range through a complex interplay between intestinal absorption, exchange with intracellular and bone storage pools, and renal tubular reabsorption. Peshawar Medical College NWFP Pakistan

• Pi is abundant in the diet. • Intestinal absorption of Pi is efficient and minimally regulated. • The kidney is a major regulator of Pi. • Kidney can increase or decrease its Pi reabsorptive capacity to accommodate Pi need. • The crucial regulatory step in Pi homeostasis is the transport of Pi across the renal proximal tubule. Peshawar Medical College NWFP Pakistan

• Survey in USA and Japan have shown that the amount of phosphorus from food is gradually increasing. • It is thought that excess amounts of phosphorus intake for long periods are a strong factor in bone impairment and ageing. • The restriction of phosphorus intake seems to be important under low calcium intake to keep QOL on high level. Peshawar Medical College NWFP Pakistan

Dietary Source • Present in all foods, Dietary deficiency is therefore unknown. • Distribution is similar to Ca++ Adequate, intake of one ensures that of the other • Best sources (Milk and milk products) • Lean meat is also a good source. Peshawar Medical College NWFP Pakistan

Recommended Dietary Allowance Infants Children Up to 6 months 240 mg 6 m – 1. 0 year 360 mg 1– 3 800 mg 4– 6 800 mg 7 – 10 800 mg Peshawar Medical College NWFP Pakistan

Recommended Dietary Allowance Adults (Male/Females) 11 – 14 1200 mg 15 – 18 1200 mg 19 – 22 800 mg 23 – 50 800 mg 51+ 800 mg Pregnant and lactating women should take an additional 400 mg. Peshawar Medical College NWFP Pakistan

Absorption and Metabolism • Mid jejunum is the main site of absorption for free PO 4. • In ileum absorption occurs via active process. • Organic phosphate in food is hydrolyzed in GIT by Pancreatic & intestinal enzymes. Peshawar Medical College NWFP Pakistan

Absorption and Metabolism • Absorption and Excretion depend upon Ca : P ratio in diet. • Excess of either one causes, an increased excretion of other. • Normally about 70% of dietary P is absorbed (For calcium 10 – 30%) Peshawar Medical College NWFP Pakistan

Absorption and Metabolism • Most of phosphorus in food is in combined form, absorption requires splitting off of PO 4 by intestinal enzymes (Phosphatases) • Factors affecting Ca++ absorption also apply to Phosphorus, like enhancement of Vit D and inhibition by binding agents i. e. Iron and Aluminium. Peshawar Medical College NWFP Pakistan

Absorption and Metabolism • Inorganic phosphorus is released and absorbed • Its absorption decreases during period of increased utilization of carbohydrate Peshawar Medical College NWFP Pakistan

Excretion • Phosphate level is regulated by urinary excretion • 2 mg / dl is the renal thresh hold • 500 mg / day is excreted in urine Peshawar Medical College NWFP Pakistan

Excretion • General factors affecting intestinal absorption • Plasma concentration and Hydrolysis of PO 4 esters by phosphatases in the kidney. • Decrease Ca++ intake will increase urinary PO 4 excretion Peshawar Medical College NWFP Pakistan

Ratio of P : Ca, Ideal 1 : 1 • Specially during the period of rapid growth (childhood), pregnancy and lactation. • Ratio in other age groups if different, have no serious adverse effect Peshawar Medical College NWFP Pakistan

In Infants • Care should be exercised as kidneys can’t handle high phosphate load. • For Prevention of hypocalcemic tetany Ca : P ratio be 1. 5 : 1 • Ratio should be reduced to 1: 1 by age 1 yr Peshawar Medical College NWFP Pakistan

Distribution • Phosphorus accounts for about 1% of TBW. • ¼th of total body mineral matter • 80 – 90% joined with Ca++ in bones and teeth (Ratio 2: 1) as a component of calcium phosphate and is being constantly deposited and liberated from bone structure. • 10 – 20% present in all cells as phosphate ion (PO=4) Peshawar Medical College NWFP Pakistan

Functions of Phosphate ions 1. Involved in formation of bones and teeth 2. Production of high enegy po 4 compounds ATP, GTP, CP 3. Synthesis of nucleotide coenzymes NAD and NADP 4. DNA and RNA synthesis, where phospho diester linkage form the back bone of the structure. Peshawar Medical College NWFP Pakistan

FUNCTION OF PHOSPHATE IONS 5. phosphorylation of glucose in glycolysis. 6. Activation and inactivation of co-enzyme by phosphorylation, TPP, PLP, HMP shunt 7. Role in blood buffer system. Peshawar Medical College NWFP Pakistan

Functions • Component of many compounds involved in many metabolic reactions. • Sugar – PO 4 linkage in DNA and RNA • Phospholipids Transport of fat in blood • Phospholipid Bilayer cell membrane transport of substances into and out of cell Peshawar Medical College NWFP Pakistan

Functions • Phosphorylation • Necessary for glucose absorption from intestine • Glucose uptake by individual cells • Resorption of glucose by kidney Peshawar Medical College NWFP Pakistan

Functions • Monosaccharides are Phosphorylated several times during metabolic break down to yield energy. • Involved in storage and release of Energy through high energy phosphate bond of ATP and ADP. Peshawar Medical College NWFP Pakistan

Functions • Essential part of body’s delicate buffer system • Many B vitamins becomes active only when combine with phosphate. Peshawar Medical College NWFP Pakistan

Hypo-Phosphatemia a. Diminished Supply: • Starvation • Malnutrition • Vit D – Deficiency b. Increased excretion / loss • Hyperparathyroidism • Hyper thyroidism • Renal defects Peshawar Medical College NWFP Pakistan

Hypo-Phosphatemia c. Intracellular shift of phosphorus • Glucose induced • Insulin induced • Respiratory alkalosis d. Electrolytes administration • Hypercalcaemia • Hypomagnesimia Peshawar Medical College NWFP Pakistan

Hypophosphatemia • Signs and Symptoms – Anorexia – Dizziness – Bone pain – Muscle weakness Peshawar Medical College NWFP Pakistan

Hyper-Phosphatemia 1. Endocrine disease – Increased growth hormones (acromegaly) – Hypo parathyroidism low calcium – Pseudo hypo parathyroidism 2. Renal Diseases • Chronic renal insufficiency • Acute renal failure Peshawar Medical College NWFP Pakistan

Hyper-Phosphatemia 3. Catabolic states • Stress or injury • Chemotherapy for malignant disease 4. Excess intake or Absorption • Laxatives or Enemas containing phosphate • Hyper vitaminosis – D Peshawar Medical College NWFP Pakistan

Hyperphosphatemia • signs and symptoms – Excessive phosphorus can bind calcium – Low calcium can cause nerve fibers to discharge repeatedly without provocation. This can lead to muscle spasms and convulsions. – Excess phosphorus and not enough calcium can lead to increased bone loss. – Increased phosphorus intake may lead to osteoporosis later in life. Peshawar Medical College NWFP Pakistan

Serum Phosphorous level • • • Plasma level in adult ---- = 3 -4 mg/dl In children------------- = 5 -6 mg/dl Occurs as free ion, almost 50% combined with proteins almost 40% and complexed with calcium and Magneessium about 10%. • Fasting levels are higher than PP Peshawar Medical College NWFP Pakistan

Clinical Importance Peshawar Medical College NWFP Pakistan

Thanks Peshawar Medical College NWFP Pakistan