Serial Dilutions Get 2 x 3 inch jewelry

Serial Dilutions

Get 2 x 3 inch jewelry bags jewelry supply store. See product # 52 -002 on second table @ http: //www. aajewelry. com/ templates/product_list_dro pdown_pix. aspx? Group. Gui d=5523 Cut piece of paper towel and insert into bag Staple across the bag such that there is space for roots to grow Add 10 to 15 seeds to each bag

Make a 0. 2 -g/m. L Na. Cl STOCK solution - Add 2 g of sodium chloride to beaker and add water to 10 m. L 0. 2 g/m. L Na. Cl Stock Solution

Get as many test tubes (minus two controls) as you have plastic jewelry bags for your experiment

Add 5 m. L of water to each of your test tubes

Add 5 m. L of Stock Solution to first test tube.

Mix the first Tube

Take 5 m. L of first tube and add to second tube

Mix

Repeat and remove 5 ml from last tube and discard

Using a 5 m. L disposable pipette 1. Add 3 m. L from stock to a bag 2. Add 3 m. L of water to another bag 3. Add the 3 m. L from each test tube to its respective bag

Stock Solution

Sto ck

Water

r ate W

0. 1 g/m. L Solution

0. 1 g/m L

0. 05 g/m. L Solution

0. 5 g/m L

Etc.

Let Grow in Dark for ~ 1 week

100 Mung Beans LD 50 90 80 70 % Dead 60 50 40 30 20 10 0 0. 0000 0. 0781 0. 1563 0. 3125 0. 6250 1. 2500 2. 5000 5. 0000 10. 000020. 0000 Na. Cl Concentration (%)