Serial Dilutions Get 2 x 3 inch jewelry
- Slides: 25
Serial Dilutions
Get 2 x 3 inch jewelry bags jewelry supply store. See product # 52 -002 on second table @ http: //www. aajewelry. com/ templates/product_list_dro pdown_pix. aspx? Group. Gui d=5523 Cut piece of paper towel and insert into bag Staple across the bag such that there is space for roots to grow Add 10 to 15 seeds to each bag
Make a 0. 2 -g/m. L Na. Cl STOCK solution - Add 2 g of sodium chloride to beaker and add water to 10 m. L 0. 2 g/m. L Na. Cl Stock Solution
Get as many test tubes (minus two controls) as you have plastic jewelry bags for your experiment
Add 5 m. L of water to each of your test tubes
Add 5 m. L of Stock Solution to first test tube.
Mix the first Tube
Take 5 m. L of first tube and add to second tube
Mix
Repeat and remove 5 ml from last tube and discard
Using a 5 m. L disposable pipette 1. Add 3 m. L from stock to a bag 2. Add 3 m. L of water to another bag 3. Add the 3 m. L from each test tube to its respective bag
Stock Solution
Sto ck
Water
r ate W
0. 1 g/m. L Solution
0. 1 g/m L
0. 05 g/m. L Solution
0. 5 g/m L
Etc.
Let Grow in Dark for ~ 1 week
100 Mung Beans LD 50 90 80 70 % Dead 60 50 40 30 20 10 0 0. 0000 0. 0781 0. 1563 0. 3125 0. 6250 1. 2500 2. 5000 5. 0000 10. 000020. 0000 Na. Cl Concentration (%)
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