PfuDNAPCR Taq DNA Polymerase Tth DNA Polymerase Ampli

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�Pfu能糾正DNA擴增（PCR）過程中產 生的錯誤，而傳統的Taq DNA Polymerase, Tth DNA Polymerase以 及Ampli. Taq, Klen. Taq等都不能進行糾 錯。

�Pfu能糾正DNA擴增（PCR）過程中產 生的錯誤，而傳統的Taq DNA Polymerase, Tth DNA Polymerase以 及Ampli. Taq, Klen. Taq等都不能進行糾 錯。

�高溫DNA聚合酶Vent, Deep. Vent, Tli, Pwo, Pfu, Ul. Tma等具有校正功能（ Proof reading），但是Pfu是目前已發 現的所有高溫DNA聚合酶中在擴增 DNA時出錯率（error rate）最低的。 它比Taq

�高溫DNA聚合酶Vent, Deep. Vent, Tli, Pwo, Pfu, Ul. Tma等具有校正功能（ Proof reading），但是Pfu是目前已發 現的所有高溫DNA聚合酶中在擴增 DNA時出錯率（error rate）最低的。 它比Taq DNA Polymerase及Ampli. Taq, Klen. Taq低10倍，比Vent和Pwo低4倍， 比Ul. Tma低30倍。

Methanogenus (產甲烷菌) Characteristic： 1. strict anaerobe 2. energy and carbon sources— H 2 /CO

Methanogenus (產甲烷菌) Characteristic： 1. strict anaerobe 2. energy and carbon sources— H 2 /CO 2 formic acid methyl alcohol methylamine acetic acid 3. metabolite—

Structure: *Cell envelope(細胞封套)： cell wall, superficial layer, vaginae, capsule *plasma membrance *protoplasm *chromosome

Structure: *Cell envelope(細胞封套)： cell wall, superficial layer, vaginae, capsule *plasma membrance *protoplasm *chromosome

Special Coenzyme: F 420 characteristic: *irradiated in the UV-light(420 nm), it turns blue&green *it

Special Coenzyme: F 420 characteristic: *irradiated in the UV-light(420 nm), it turns blue&green *it can be decomposed by the aerobic bacteria

application Organic waste Nutural gas Methanogenu s CH 4 coal

application Organic waste Nutural gas Methanogenu s CH 4 coal

圖片出處 火山 http: //www. nipic. com/show/1/65/fe 47 c 9 fa 292 bf 9 de.

圖片出處 火山 http: //www. nipic. com/show/1/65/fe 47 c 9 fa 292 bf 9 de. html 古菌. 黃石公園火山. 系統發生樹圖 http: //en. wikipedia. org/wiki/Archaea

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