200 1000 L 278 This is a Micropipette

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200 -1000 μL 278 This is a Micropipette it is used to accurately transfer

200 -1000 μL 278 This is a Micropipette it is used to accurately transfer small quantities of liquid. ﻳﺘﻢ ﺍﺳﺘﺨﺪﺍﻣﻬﺎ ﻟﻨﻘﻞ Micropipette ﻫﺬﻩ ﺍﻟـ . ﻛﻤﻴﺎﺕ ﺻﻐﻴﺮﺓ ﻣﻦ ﺍﻟﺴﺎﺋﻞ ﺑﺪﻗﺔ On this model the desired volume can be adjusted within the range of microliters (μL). ﻓﻲ ﻫﺬﺍ ﺍﻟﻤﻮﺩﻳﻞ ﻳﺘﻢ ﺿﺒﻂ ﺍﻟﺤﺠﻢ ﺍﻟﻤﻄﻠﻮﺏ . ( ﻣﻴﻜﺮﻭﻟﺘﺮ microliters ) ﺑﻮﺣﺪﺓ

200 μL Some Micropipettes have preset volumes and cannot be adjusted. This pipette will

200 μL Some Micropipettes have preset volumes and cannot be adjusted. This pipette will only deliver 200μL. ﻟﻬﺎ ﺃﺤﺠﺎﻡ ﻣﺤﺪﺩﺓ Micropipettes ﺑﻌﺾ ﺍﻟـ . ﻣﺴﺒﻘﺎ ﻭﻻ ﻳﻤﻜﻦ ﺗﻌﺪﻳﻠﻬﺎ

278 How does it work? It works much like a syringe that would deliver

278 How does it work? It works much like a syringe that would deliver an injection. Inside there is a spring loaded piston that moves up and down. ﻛﻴﻒ ﺗﻌﻤﻞ؟ . ﺗﻌﻤﻞ ﺗﻤﺎﻣ ﻣﺜﻞ ﺣﻘﻨﺔ ﺍﻟﺪﻭﺍﺀ . ﻓﻲ ﺍﻟﺪﺍﺧﻞ ﻳﻮﺟﺪ ﻛﺎﺑﺴﺔ ﺗﺘﺤﺮﻙ ﺻﻌﻮﺩﺍ ﻭﻫﺒﻮﻃﺎ

278 278μL -The second stop is extra travel used for certain circumstances like evacuating

278 278μL -The second stop is extra travel used for certain circumstances like evacuating the tip, or drawing up more than the volume indicated on the pipette ﻭﺣﻴﻦ ﺗﻀﻐﻄﻴﻦ ﺣﺘﻰ ﺍﻟﻤﺤﻄﺔ ﺍﻟﺜﺎﻧﻴﺔ ﺗﺰﻳﺪ ﺍﻟﻜﻤﻴﺔ ﻋﻦ ﺍﻟﻜﻤﻴﺔ ﺍﻟﻤﻘﺎﺳﺔ – ﻭﺗﺴﺘﺨﺪﻡ ﻓﻲ ﺣﺎﻻﺕ ﻣﻌﻴﻨﺔ ﻓﻘﻂ 278 - Extra volume (? μL)

Single Channel- Multi-Channel

Single Channel- Multi-Channel

Examples of Manual and Electronic Manual single channel Manual multi - channel Electronic single

Examples of Manual and Electronic Manual single channel Manual multi - channel Electronic single channel Electronic multichannel

Attaining a Sample 278 HOLD STEP ONE – press plunger to first stop and

Attaining a Sample 278 HOLD STEP ONE – press plunger to first stop and hold. ﺍﺿﻐﻂ ﺍﻟﻤﻜﺒﺲ ﻟﻠﻤﺤﻄﺔ ﺍﻷﻮﻟﻰ ﻣﻊ . ﺍﻻﺳﺘﻤﺮﺍﺭ

Attaining a Sample Removed tip from sample before the plunger was all the way

Attaining a Sample Removed tip from sample before the plunger was all the way home CORRECT Likely allowed the plunger to move to home too quickly.

278 Delivering the Sample KEEP the pipette VERTICAL at all times STEP FOUR –

278 Delivering the Sample KEEP the pipette VERTICAL at all times STEP FOUR – insert tip into the area you wish to deliver your sample. (in this case a gel for DNA fingerprinting) ﺿﻌﻲ ﻃﺮﻑ ﺍﻟﻤﺎﺻﺔ ﻓﻲ ﻗﻤﺔ ﺍﻟﻤﻨﻄﻘﺔ ﺍﻟﺘﻲ ﺗﺮﻏﺒﻴﻦ ﻓﻲ ﺗﺤﻤﻴﻞ )ﻓﻲ ﻫﺬﻩ ﺍﻟﺤﺎﻟﺔ ﺍﻟﺠﻞ. ﻋﻴﻨﺘﻚ ﺑﻬﺎ ( ﻟﺒﺼﻤﺔ ﺍﻟﺤﻤﺾ ﺍﻟﻨﻮﻭﻱ

Delivering the Sample HOLD STEP FIVE – depress the plunger slowly to the first

Delivering the Sample HOLD STEP FIVE – depress the plunger slowly to the first stop, then continue to the second stop, this will evacuate the entire contents of the tip. And HOLD 278 �� ������ ���� � �������� ��� ������� ��� KEEP the pipette VERTICAL at all times

Delivering the Sample 278 HOLD KEEP the pipette VERTICAL at all times STEP SIX

Delivering the Sample 278 HOLD KEEP the pipette VERTICAL at all times STEP SIX – While still holding the plunger at the second stop. Withdraw the tip from the well. ����� �� ������ �� ��. ������� �� ����

Delivering the Sample STEP SEVEN – Allow the plunger to return home. 278 �������

Delivering the Sample STEP SEVEN – Allow the plunger to return home. 278 ������� ��� �� ���� KEEP the pipette VERTICAL at all times

Discarding the Tip STEP EIGHT – Place tip into the opening of the waste

Discarding the Tip STEP EIGHT – Place tip into the opening of the waste container, then depress the tip ejector. Tip Ejector 278 Be sure to use a new tip each time. WASTE KEEP the pipette VERTICAL at all times

Things to AVOID !! 278 Never use a pipette without a tip.

Things to AVOID !! 278 Never use a pipette without a tip.

HAPPY PIPETTING

HAPPY PIPETTING