Introduction to the Microscope Care Parts Focusing Always

Introduction to the Microscope §Care §Parts §Focusing

• • • Always carry with 2 hands Only use lens paper for cleaning Do not force knobs Always store covered Keep objects clear of desk and cords

Eyepiece: Contains a magnifying glass – ours magnifies 10 X

Arm: Supports the body tube; carry the scope with one hand on this and one on the base

Revolving Nosepiece: Holds the objectives and can be rotated to changed the magnification Scanning = 4 X Low power = 10 X High power = 40 X

Objective Lenses: Provides magnification: • scanning objective • low-power objective • high-power objective

Coarse Adjustment: Moves the body tube to focus the image; moves quickly

Fine Focus: Moves the body tube slightly to sharpen the image; moves slowly

Stage: Supports the slide being observed

Stage Clips: Hold the slide in place

Diaphragm: Opens and closes the diagram; allows more or less light to come through

Light: Produces light so you can see through slide

Base: Supports the microscope

Eyepiece Body Tube Revolving Nosepiece Objective Lens Stage Clips Diaphragm Light Arm Stage Coarse Focus Fine Focus Base

• Place the Slide on the Microscope • Use Stage Clips • Allows start with scanning objective • Look into the Eyepiece • Use the Coarse Focus

• Follow steps to focus using low power • Click the nosepiece to the high power objective • Do NOT use the Coarse Focusing Knob on high power! • Use the Fine Focus Knob to bring the slide into focus

• Click the nosepiece to the scanning objective • Use the Coarse Focusing Knob to move lens all the way down towards stage • Wrap cord around base • Replace dust cover

Calculating Total Magnification Multiply the eyepiece (10 X) and the objective lens magnification (varies) Example: Objective lens magnifies 50 X. What is the total magnification?

Mystery cells -Eukaryotes