Introduction to the Microscope Care Parts Focusing Wet

Introduction to the Microscope §Care §Parts §Focusing §Wet mounts §Calculating magnification

Eyepiece Body Tube Revolving Nosepiece Objective Lens Stage Clips Diaphragm Light Arm Stage Coarse Focus Fine Focus Base

• Always carry with 2 hands- one hand on arm – other on base • Only use lens paper for cleaning • Do not force knobs

1/Use the coarse adj. to raise the body tube. 2/Turn the light on 3/Place slide on stage using stage clips 4/Position the lowest (shortest) objective into place 5/Without looking in the eyepiece, raise the stage to be very close to the objective but do not let them touch!

6/ Look through the eyepiece 7/Using the course adj. , bring it into focus 7/ Use fine adj. to get into focus further (you may have to use the fine adj. only if in using highest obj. ) 8. Move slide around if need – still keeping it clipped

Making a wet mount 1/Place specimen on slide 2/Place a drop of water on slide 3/Place cover slip on slide using the angle technique 4/Remove air bubbles by tapping gently with finger or pencil eraser 5/Dry excess water by taking a paper towel and dabbing the outside

To make a stained slide • 1/Make a wet mount with water and coverslip • 2/Place a drop of stain at one end of the cover slip • 3/ hold a piece of paper towel at the other end of cover slip - this will draw the stain across the speciman

Calculating Magnification • • Eyepiece = 10 x Scanning power obj = 4 x Low obj = 10 x Highest obj = 40 x • Ex. – eyepiece x low = 4 x x 10 x = 40 x total