Introduction to the Microscope Care Parts Focusing Always

Introduction to the Microscope §Care §Parts §Focusing

• • • Always carry with 2 hands Only use lens paper for cleaning Do not force knobs Always store covered Keep objects clear of desk and cords

Parts of a Compound Microscope

Eyepiece Body Tube Revolving Nosepiece Objective Lens Stage Clips Diaphragm Light Arm Stage Coarse Focus Fine Focus Base

Compound Microscope • A microscope is a very powerful magnifying glass • A microscope helps you see things like cells up close

Eyepiece • View the specimen through the eyepiece

Stage Clips & Objectives • Stage clips hold the slide in place • Low power objective is used to focus the microscope (short & fat) • High power objective is used to view details of a specimen

Coarse Adjustment, Fine Adjustment, & Base • Coarse adjustment focuses adjustment • Fine adjustment fine tunes & gives detailed focus(usually smaller than coarse adjustment knob) • Base is where the microscope rests

Stage • Stage is part where the slide rests • Mirror (or light source) directs light upwards onto the slide.

Diaphragm • Diaphragm allows light in

Nosepiece • Nosepiece is the rotating device that holds the objectives (lenses)

Arm • Arm is the part where you carry the microscope

Can you name the parts of a compound microscope?

Answers 1) base 2) mirror (light source) 3) diaphragm 4) stage 5) stage clips 6) low power objective lens 7) high power objective lens 8) nosepiece 9) arm 10) fine focus knob 11) body tube 12) coarse focus knob 13) eyepiece

Types of Microscopes

Light microscope • (for small objects similar to the way binoculars magnify objects far away. )

The Compound Light Microscope http: //www. cas. muohio. edu/~mbi-ws/microscopes/compoundscope. html

• May have many lenses that magnify the object • Eyepiece = 10 x magnification • Objective = 10 x (low) or 40 X (high)

• Total magnification= • eyepiece X objective • ex: 10 x X 40 x = 400 x total magnification

Compound Light Microscope • Powered by Light & Lenses a) Powers of Magnification • Ocular Lens= _____ • Low Power Objective= _______ • Medium Power Objective= _______ • High Power Objective= _______ • Calculating Total Magnification= ______ b) Resolution c) Parts

Stereomicroscope • To look at large things that light cannot pass through (one eyepiece for each eye)

Stereomicroscopes http: //scientificsonline. com/product. asp_Q_pn_E_3119600 http: //www. martinmicroscope. com/Microscope. Pages/Stereomicroscopes. htm

Stereomicroscopes • 3 - D image • can only magnify 10 x to 600 x

Electron Microscope • can magnify more than 500, 000 x

House Fly • http: //www. pbrc. hawaii. edu/bemf/microangela/hfly. htm

• Place the Slide on the Microscope • Use Stage Clips • Click Nosepiece to the lowest (shortest) setting • Look into the Eyepiece • Use the Coarse Focus

• • Follow steps to focus using low power Click the nosepiece to the longest objective Do NOT use the Coarse Focusing Knob Use the Fine Focus Knob to bring the slide into focus What can you find on your slide?