Denaturation of Enzymes Factors that Denature Enzymes n

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Denaturation of Enzymes

Denaturation of Enzymes

Factors that Denature Enzymes n n Enzymes are proteins. Denaturation: n n n changes

Factors that Denature Enzymes n n Enzymes are proteins. Denaturation: n n n changes the structure of the protein stops enzyme activity Enzymes denatured by: n n heat p. H salts enzyme inhibitors B-3. 03 -- Denaturation of Enzymes 2

Heat n Enzymatic reaction rates increase as temperature increases. n n Denaturation occurs at

Heat n Enzymatic reaction rates increase as temperature increases. n n Denaturation occurs at 104°F or hotter. Too much heat slows and stops enzyme activity. n n Heating can destroy enzymes that alter food quality. Example -- blanching vegetables to prevent spoilage during frozen storage. B-3. 03 -- Denaturation of Enzymes 3

p. H n p. H and rate of enzymatic reaction n n p. H

p. H n p. H and rate of enzymatic reaction n n p. H and denaturation of enzymes n n n Specific to each enzyme Optimal p. H range. Most enzymes will denature if p. H is too high or too low. Changing the p. H can speed up, slow, or stop enzymatic reactions n Lemon juice to apples B-3. 03 -- Denaturation of Enzymes 4

Salts n Salt is commonly used in food production n n Binds to enzymes

Salts n Salt is commonly used in food production n n Binds to enzymes in food that cause spoilage Changes the water activity B-3. 03 -- Denaturation of Enzymes 5

Enzyme Inhibitors n Substance that will prevent the enzymesubstrate complex n n Naturally occurring

Enzyme Inhibitors n Substance that will prevent the enzymesubstrate complex n n Naturally occurring n n n Enzymatic reactions slowed or stopped. Egg whites Pesticides that are naturally part of a plant structure Synthetic compounds B-3. 03 -- Denaturation of Enzymes 6