Circadian rhythm March 14 1088 tongli zhang Circadian

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Circadian rhythm • March 14 1088 • tongli zhang

Circadian rhythm • March 14 1088 • tongli zhang

Circadian rhythm

Circadian rhythm

Circadian rhythms in different organisms

Circadian rhythms in different organisms

 • Control network in mammalian

• Control network in mammalian

Circadian rhythms in Cyanobacyteria

Circadian rhythms in Cyanobacyteria

cyanobacteria

cyanobacteria

Their interaction • Kai. A, Kai. B and Kai. C interact with each other,

Their interaction • Kai. A, Kai. B and Kai. C interact with each other, forming large complexes in vivo. • Kai. C is the core of the complex. • The complex is mainly at night.

kai. C

kai. C

Kai. A • kai. A enhances Kai. C phosphorylation • Kai. A has two

Kai. A • kai. A enhances Kai. C phosphorylation • Kai. A has two domains- N-terminal is a “pseudo-receive” domain • N-terminal domain of Kai. A does not promote Kai. C autophosphorylation • Kai. A from anabaena even does not have a N-terminal domain • However, mutation of N-terminal domain of Kai. A lengthen the circadian period

Kai. A 2 • Kai. A C-terminal binds Kai. C and promotes its phosphorylation

Kai. A 2 • Kai. A C-terminal binds Kai. C and promotes its phosphorylation • One Kai. A is enough to enhance the phosphotylation of one Kai. C hexamer

Kai. A 3 • Kai. A exists as a domain-swapped homodimer • Kai. A's

Kai. A 3 • Kai. A exists as a domain-swapped homodimer • Kai. A's pseudo-receiver domain • The C-terminal domain of Kai. A, C-Kai. A, is all ahelical and adopts a novel dimeric structure.

Kai. A 4 open and close • kai. A dimer N of one and

Kai. A 4 open and close • kai. A dimer N of one and C of another interact • This is closed • When the interaction is broken- Kai. A dimer is open • Closed is supposed to be the active form

kai. C binding with Kai. A

kai. C binding with Kai. A

kai. B • Positively charged residues R 23 and K 75

kai. B • Positively charged residues R 23 and K 75

kai. C binds with Kai. B • The potentials (positive, blue; negative, red)

kai. C binds with Kai. B • The potentials (positive, blue; negative, red)

A rotary clock

A rotary clock

Happy everyone? • • • What about 1. entrainment? 2. temperature compensation? 3. fitness?

Happy everyone? • • • What about 1. entrainment? 2. temperature compensation? 3. fitness? 4. cancer development?

Way to solutions.

Way to solutions.