AGAR PLATES v Sterile petri dish containing agar

AGAR PLATES

v. Sterile petri dish containing agar and usually nutrients v. Used to culture microorganisms

ABOUT AGARS “BLOOD” REGULAR “SUGAR” AGARS ADDITIVES PREPOURED SUSPEND IN H 20

AGAR DEFINED UNDEFINED SELECTIVE PERMISSIVE Chemicals required Natural products Certain organisms Many organisms

v. Agar plates are used as a medium for growing microorganisms, usually bacteria v. Agar plates contain a gelatin, this is because this substance usually isn't degraded by bacteria. This is why some plates contain different material v. Used to help make decisions with prescribing medications or just for identification WHY?

v. Commonly used due to high number of microbes, fungus and bacteria that can be grown (not selective) v. Beef broth and extracts from yeast make it non selective NUTRIENT AGAR

v. Contains 5% blood (sheep) v. Can be contaminated from human contact Blood Agar

v. Eosin Methylene Blue v. Isolates and differentiation of gram negative Bacilli v. One of the many agars that are irritating to the eyes, skin and respiratory system EMB AGAR

v. Most of agar is made of chocolate, 1% hemoglobin (sheep) v. Usually for Haemophilus growth, fastidious bacteria v. Can be contaminated by human contact CHOCOLATE AGAR

v. Usually for growth of gram negative v. E. Coli grow into red colonies v. AGAR POWDER: v. Sugar Lactose v. Non Sugar MACCONKEY AGAR

10 g lactose 20 g peptone 5 g bile salts "pinch" or small amount of 1 mg/m. L Bromcresol purple or Crystal violet solution. 15 g agar 1 L distilled water

v. Kills gram - and + v. Contains neomycin antibiotic, which is naturally made from Streptomyces fradiae v. Relatively toxic but can cause allergic reactions in some people NEOMYCIN AGAR

AGAR TY[ES LB (Luria Bertani) Agar Non-nutrient Agar Sabouraud Agar Thayer-Martin Agar Tryptic Soy Agar XLD Agar Xylose lysine Deoxycholate Mannitol salt Potato dextrose Millers LB

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