Introduction to the NGC Chromatographic System Protein purification

Introduction to the NGC Chromatographic System

Protein purification systems – yesterday & today • Simplified, a chromatography system is just: – Pumps – Valves – (Columns) – Detector(s) – Fraction Collector 2

NGC System Capabilities Bulletins 6286, 6326, and 6608 3

A chromatography platform that aligns to your needs Quest™ & Quest™ Plus Workhorse system for the purification of proteins peptides and nucleic acids Scout™ & Scout™ Plus Discover ™ All purpose purification system with buffer scouting Versatile automated purfication system for advanced method development 4

Expandable system that grows with you Get what you need today with a single, scalable platform that grows and adapts to your throughput and purification needs over time Tier Expansion 5

Plug-n-Play Modular Design A truly modular design § Plug-n-play modules can be easily moved within the system § New modules can be added to expand system capabilities as your purification needs change Plug-n-Play Modules Note system must be switched off before modules are removed 6

Chrom. Lab Software Highlights • • • Touchscreen Intuitive Method Templates – Column Library – Scouting – Multi-D • • Import GE Unicorn SW and Bio-Rad Duo. Flow files Analysis made easy – – • • 7 One click peak integration Easy overlay Fraction heat map Concentration determination User Management Edition and Security Edition FREE Bulletin 6675

The Heart of the System - Pumps • Two versions available, 10 and 100 m. L/min • Buffer blending module allows for double flow and simplified buffer preparation 8 8

Inlets – Great for Automation • 9 A buffer inlet valve provides automated buffer switching. 9

Sample Loading – Volume is Key • Volumes >10 ml use a sample loading pump with air sensor. • Smaller volumes should be done with loops. 10 10

Column Switching Valve • • 11 Allows reverse flow CSV can also serve as a “loop multiplier” allowing for up to 5 sample loops 11

Superman’s Eyes Human Eyes Detectors – The “eyes” of the system 12 • Single wavelength for labs doing simple protein OR DNA purification • Multi-Wavelength is great for labs that: • Purify Protein/DNA complexes • Purify a fluorescently labeled protein • Purify a protein with no signal at 280 nm 12

Outlet – Large Volume Fractionation • Useful for labs doing large volume work • Able to save the “flow through” in one large container rather using the Bio. Frac 13 13

NGCTM system capabilities System Pump Sample Inj. Valve Column Valve Single-WL Detector - 10 ml/min, 25. 2 MPa - No re-plumbing - Up to 5 col. - 255/280 nm - Int. Bypass Conductivity Monitor - Reverse Flow - 0. 01 – 999 m. S/cm - Pre- & Delta Col. Pressure Multi-WL Detector - 100 ml/min, 10 MPa Buffer Inlet Valve (A/B) - Up to 8 Inlets each Buffer Blending Valve - p. H Scouting - Doubles pump flow with gradient Sample Pump - Ideal for Affinity - Integrated - 100 ml/min, 10 MPa - 190 -800 nm (UV/Vis) - Int. Press. Sensor - 2, 5 & 10 mm Path lengths - Up to 4 simultaneous Bio. Frac. TM Fraction Collector - Unlimited Options - 180 x 12 mm tubes - 168 x 1. 5 ml tubes - 120 x 15 mm tubes - 24 x 30 mm tubes - 4 x 96, 48 -, 24 - or 12 position micro-plates Air Sensor p. H Probe & Valve - 20 x unlimited vol. adaptor - Protects Column - In situ calibration -Outlet valve - 14 Up to 8 Inlets (7 Buffers, 1 Sample) - Integrated bypass

Standout features • Point-to-Plumb™ • Pay for only what you need • Infinitely configurable – Tier expansion – Columns mount anywhere – Modules can go anywhere • Free software—for life! • Ten-second module swapping • Highly intuitive software 15