Essential and toxic microelements in grains of Fusariuminfected
- Slides: 30
Essential and toxic microelements in grains of Fusariuminfected maize and wheat genotypes: ICP-MS analysis Zsuzsanna Kolbert, Attila Ördög, Ágnes Vashegyi, László Erdei Two countries, one goal, joint success!
Materials and methods Plant material: • • 2 maize genotypes (two sampling times) 13 wheat genotypes Infection: • Fusarium graminearum • Fusarium culmorum • Fusarium verticillioides Sample preparation: • 500 – 1000 mg from each sample • microwave digestion in nitric acid and hydrogen peroxide • dilution to 200 - 400 X Measurement: • Microelement analysis (Mn, Fe, Co, Ni, Cu, Zn, As, Se, Mo, Cd, Pb) by ICPMS device Two countries, one goal, joint success!
The advantages of an ICP-MS • More than 1000 samples in one week (up to 250 samples/day) • More than 15 000 data from the analysis • Lot of information in a few seconds (less than a second per element) • User-friendly software (Plasma. Lab), which makes the data analysis easier and faster Two countries, one goal, joint success!
Microelement contents in the two maize genotypes GK-Sarolta GK-Boglár Two countries, one goal, joint success!
Mn content in maize genotypes 25 µg/g dry weight 20 15 Control F. g. F. c. 10 F. v. 5 0 1 st sampling Sarolta 2 nd sampling Sarolta 1 st sampling Boglár 2 nd sampling Boglár Two countries, one goal, joint success!
Fe content in maize genotypes 50 µg/g dry weight 40 30 Control F. g. F. c. 20 F. v. 10 0 1 st sampling Sarolta 2 nd sampling Sarolta 1 st sampling Boglár 2 nd sampling Boglár Two countries, one goal, joint success!
Co content in maize genotypes 40 ng/g dry weight 30 Control F. g. 20 F. c. F. v. 10 0 1 st sampling Sarolta 2 nd sampling Sarolta 1 st sampling Boglár 2 nd sampling Boglár Two countries, one goal, joint success!
Ni content in maize genotypes 2500 ng/g dry weight 2000 1500 Control F. g. F. c. 1000 F. v. 500 0 1 st sampling Sarolta 2 nd sampling Sarolta 1 st sampling Boglár 2 nd sampling Boglár Two countries, one goal, joint success!
Cu content in maize genotypes 7 6 µg/g dry weight 5 4 Control F. g. F. c. 3 F. v. 2 1 0 1 st sampling Sarolta 2 nd sampling Sarolta 1 st sampling Boglár 2 nd sampling Boglár Two countries, one goal, joint success!
Zn content in maize genotypes 80 70 60 µg/g dry weight 50 Control F. g. 40 F. c. F. v. 30 20 10 0 1 st sampling Sarolta 2 nd sampling Sarolta 1 st sampling Boglár 2 nd sampling Boglár Two countries, one goal, joint success!
As content in maize genotypes 25 ng/g dry weight 20 15 Control F. g. F. c. 10 F. v. 5 0 1 st sampling Sarolta 2 nd sampling Sarolta 1 st sampling Boglár 2 nd sampling Boglár Two countries, one goal, joint success!
Se content in maize genotypes 1400 1200 ng/g dry weight 1000 800 Control F. g. F. c. 600 F. v. 400 200 0 1 st sampling Sarolta 2 nd sampling Sarolta 1 st sampling Boglár 2 nd sampling Boglár Two countries, one goal, joint success!
Mo content in maize genotypes 1400 1200 ng/g dry weight 1000 800 Control F. g. F. c. 600 F. v. 400 200 0 1 st sampling Sarolta 2 nd sampling Sarolta 1 st sampling Boglár 2 nd sampling Boglár Two countries, one goal, joint success!
Cd content in maize genotypes 30 25 ng/g dry weight 20 Control F. g. 15 F. c. F. v. 10 5 0 1 st sampling Sarolta 2 nd sampling Sarolta 1 st sampling Boglár 2 nd sampling Boglár Two countries, one goal, joint success!
Pb content in maize genotypes 300 250 ng/g dry weight 200 Control F. g. 150 F. c. F. v. 100 50 0 1 st sampling Sarolta 2 nd sampling Sarolta 1 st sampling Boglár 2 nd sampling Boglár Two countries, one goal, joint success!
Microelement contents in 13 different wheat genotypes • • • • Manastur - TM Sanandrei - TM Faget-TM Pietroasa- TM Lovrin -TM Aldesti -TM Dudestii Vechi -TM Horea - Ar Siria - Ar Recas - Ar Semlac - Ar Paulis - Ar Barzava - Ar Two countries, one goal, joint success!
Mn content in different wheat genotypes 100 µg/g dry weight 75 50 Grain Chaff 25 0 Manastur. Sanandrei. Faget-TMPietroasa- Lovrin - Aldesti - Dudestii Horea - Ar Siria - Ar Recas - Ar Semlac - Paulis - Ar. Barzava - TM TM Vechi -TM Ar Ar Two countries, one goal, joint success!
Fe content in different wheat genotypes 250 µg/g dry weight 200 150 Grain Chaff 100 50 0 Manastur. Sanandrei. Faget-TMPietroasa- Lovrin - Aldesti - Dudestii Horea - Ar Siria - Ar Recas - Ar Semlac - Paulis - Ar. Barzava - TM TM Vechi -TM Ar Ar Two countries, one goal, joint success!
Co content in different wheat genotypes 125 ng/g dry weight 100 75 Grain Chaff 50 25 0 Manastur. Sanandrei. Faget-TMPietroasa- Lovrin - Aldesti - Dudestii Horea - Ar Siria - Ar Recas - Ar Semlac - Paulis - Ar. Barzava - TM TM Vechi -TM Ar Ar Two countries, one goal, joint success!
Ni content in different wheat genotypes 700 600 ng/g dry weight 500 400 Grain Chaff 300 200 100 0 Manastur. Sanandrei. Faget-TMPietroasa- Lovrin - Aldesti - Dudestii Horea - Ar Siria - Ar Recas - Ar Semlac - Paulis - Ar. Barzava - TM TM Vechi -TM Ar Ar Two countries, one goal, joint success!
Cu content in different wheat genotypes 16 µg/g dry weight 12 8 Grain Chaff 4 0 Manastur. Sanandrei. Faget-TMPietroasa- Lovrin - Aldesti - Dudestii Horea - Ar Siria - Ar Recas - Ar Semlac - Paulis - Ar Barzava - TM TM Vechi -TM Ar Ar Two countries, one goal, joint success!
Zn content in different wheat genotypes 100 µg/g dry weight 75 50 Grain Chaff 25 0 Manastur. Sanandrei. Faget-TMPietroasa- Lovrin - Aldesti - Dudestii Horea - Ar Siria - Ar Recas - Ar Semlac - Paulis - Ar. Barzava - TM TM Vechi -TM Ar Ar Two countries, one goal, joint success!
As content in different wheat genotypes 250 ng/g dry weight 200 150 Grain Chaff 100 50 0 Manastur. Sanandrei. Faget-TMPietroasa- Lovrin - Aldesti - Dudestii Horea - Ar Siria - Ar Recas - Ar Semlac - Paulis - Ar. Barzava - TM TM Vechi -TM Ar Ar Two countries, one goal, joint success!
Se content in different wheat genotypes 400 ng/g dry weight 300 200 Grain Chaff 100 0 Manastur. Sanandrei. Faget-TMPietroasa- Lovrin - Aldesti - Dudestii Horea - Ar Siria - Ar Recas - Ar Semlac - Paulis - Ar. Barzava - TM TM Vechi -TM Ar Ar Two countries, one goal, joint success!
Mo content in different wheat genotypes 1600 ng/g dry weight 1200 800 Grain Chaff 400 0 Manastur. Sanandrei. Faget-TMPietroasa- Lovrin - Aldesti - Dudestii Horea - Ar Siria - Ar Recas - Ar Semlac - Paulis - Ar. Barzava - TM TM Vechi -TM Ar Ar Two countries, one goal, joint success!
Cd content in different wheat genotypes 100 ng/g dry weight 75 50 Grain Chaff 25 0 Manastur. Sanandrei. Faget-TMPietroasa- Lovrin - Aldesti - Dudestii Horea - Ar Siria - Ar Recas - Ar Semlac - Paulis - Ar. Barzava - TM TM Vechi -TM Ar Ar Two countries, one goal, joint success!
Pb content in different wheat genotypes 7000 6000 ng/g dry weight 5000 4000 Grain Chaff 3000 2000 1000 0 Manastur. Sanandrei. Faget-TMPietroasa- Lovrin - Aldesti - Dudestii Horea - Ar Siria - Ar Recas - Ar Semlac - Paulis - Ar. Barzava - TM TM Vechi -TM Ar Ar Two countries, one goal, joint success!
Pb content in different wheat genotypes 500 ng/g dry weight 400 300 Grain 200 100 0 Manastur. Sanandrei. Faget-TMPietroasa- Lovrin - Aldesti - Dudestii Horea - Ar Siria - Ar Recas - Ar Semlac - Paulis - Ar. Barzava - TM TM Vechi -TM Ar Ar Two countries, one goal, joint success!
Summary Maize genotypes: • Infections by all Fusarium strains increased the concentrations of microelements (Mn, Fe, Ni, Cu) and toxic metals (Cd, Pb) in the grains Wheat genotypes: • Mn and Ni concentration were high in Barzava-Ar • Fe, Co, As content were higher in the chaff compared to grains in all genotypes • In the chaff of Horea-Ar high Pb and As concentrations were measured protection for the grain Two countries, one goal, joint success!
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