DIRECTIONS to follow ASAP Read pages 830 831

DIRECTIONS to follow ASAP: • Read pages 830 -831 silently • On a new sheet of paper, write the title “Proper Use of the Compound Light Microscope” • Write a PHRASE summarizing each of the 10 steps listed (use bullet points…short and sweet!) • You will have 10 minutes to finish silently!

Body Tube Nosepiece Objectives Ocular lens (Eyepiece) Arm Stage Clips Coarse Adjustment Diaphragm Light Fine Adjustment Base

Focuses Bodyocular Tube lens Rotates Objective Lenses Nosepiece Scanning Objective (4 x) Objectives Where you Ocular lens View the (Eyepiece) Specimen! Arm Low Objective (10 x) Hi Power Objective (40 x) Hold Stage Slide Clips Controls Diaphragm light! Light Stage Moves stage up/down; Coarse Adjustment Use to scan for specimen Finein. Adjustment Use high power for Fine Adjustments Base

Microscope Lenses • Eyepiece – Holds Ocular Lens – Magnification of 10 X • Scanning (red) Objective Lens – Magnification of 4 x – Used for Scanning – Use Coarse Focus Knob • Low Power (yellow) Objective Lens – Magnification of 10 X – Used for Scanning – Use Coarse Focus Knob • High Power (blue) Objective Lens – Magnification of 40 X – Used for Fine Focusing • Seeing the details – Use Fine Focus Knob ONLY • Do NOT use Coarse Focus Knob

The Light Source sends light through the slide. The Iris Diaphragm regulates the amount of light from the light source that passes through the slide. The coarse adjustment should only be used in scanning (4 x) and low power (10 x) objectives. It is used for seeing the slide. You should use the fine adjustment while in the high power objective. It is used for detailed focus.

The stage clips hold the slide and are located on the stage which is moved to view different areas of the slide and specimen. All of the objectives are held on the nosepiece which rotates to change the power of magnification. The scanning lens is usually 4 x magnification and is used for initial scanning and specimen location on the slide.

The low or medium power objective lens is usually 10 x magnification and is also used for scanning. The high power lens is usually 40 x or 100 x and is used for detailed viewing of a specimen.

Brain Pop- Microscopes

Directions • Get out your Ch. 3 -1 Directed reading HW • Study it silently for 5 minutes before the quiz • Finished with Quiz? STAY SEATED…leave dividers UP.

What’s my power? To calculate the power of magnification, MULTIPLY: Ocular Lens Power x Objective Lens Power = total magnification What is the total power of magnification for the Ocular Lens (shown) and the 40 x Objective Lens (shown)? (Write calculations & answer)

Comparing Powers of Magnification We can see better details with higher the powers of magnification, but we cannot see as much of the image. Which of these images would be viewed at a higher power of magnification?

SAFETY: Do’s and Don’ts of Microscope Labs! • USE TWO HANDS – Always carry microscopes with one hand on the ARM and the other UNDER the BASE! BASE

Do’s and Don’ts • NEVER use Coarse Focus Knob with High Power Lens…it Breaks Slides! • “Ooops, I broke a slide!” – Accidents Happen. Don’t FREAK OUT! OUT – Don’t try to clean it up by yourself! – Raise your hand a teacher will help you!

Cleaning UP! • Always do 5 Things! 1. 2. 3. 4. 5. Lower stage Remove slide Wrap cord Replace plastic cover (7 th period) Put microscope back in designated area with arm out

Spooky Cell 4 Stretch me and work me to build more bulk. Soon you will look like the incredible hulk!

Let’s act it out. . . 1 – PRETEND to Turn on the microscope and then rotate the nosepiece to click the (low power) red-banded objective into place. 2 – Place an IMAGINARY slide on the stage and secure it using the stage clips. Use the coarse adjustment knob (large knob) to get it the image into view and then use the fine adjustment knob (small knob) to make it clearer. 3 – Once you have the image in view, rotate the nosepiece to view it under different powers. Be careful with the largest objective! Sometimes there is not enough room and you will not be able to use it! 4 – When you are done, turn off the microscope and put up the slides you used.

Directions… Use the bag of cards and match up the microscope part to its function. Work with the person next to you!