Cell signal transduction diseases moon rover Zhao Mingyao
- Slides: 56
Cell signal transduction & diseases moon rover Zhao Mingyao BMC. ZZU
cell signal transduction signal cell specific response Proliferation Differentiation Metabolism Function Stress Apoptosis or loss disease
1. Signal constitution site feature (1)Extracellu Chemical: lipid-soluble & water-soluble lar ~ Physical: mechanical, light, electrical (2)Intracellul Enzyme, protein, ion, lipid ar ~
(3)Second messenger c. AMP, c. GMP Ca 2+ DAG(1, 2 -diacylglycerol) NO, CO, ? ceramide, phosphocholine allosteric agent
(4) Enzyme components ①Phospholipase(PL) : PLA 2, C, D, SMase (sphingomyelinase) ②Phosphatidylinositol kinase: PI-3 K, PI-4 K, PI-5 K / PTEN ③GP (Tripolymer & Small ) : GP(G ) = GTPase ④Protein kinase & phosphatase: PSTK & TPK or PTK ⑤AC, GC/ cyclic nucleotide phosphodiesterase: phosphatase and tensin homolog deleted on chromosome ten (PTEN)
(5) Receptor and its function 1. ionotropic ~: + neurotransmitter , ion 2. GPCR: metabolism , function modulation 3. ~ TPK: +insulin, GH 4. TPK-linking ~ : cytokine, antigen, some CAM 5. PSTK ~ : TGF-β 6. TNF ~ : apoptosis, NF-k. B 7. Guanylyl Cyclase ~: vasodilation, excreting Na+ urine 8. CAM : communication between cells 9. Nuclear ~: transcription regulatory factor
2. Signal transduction pathway channel Effect protein Transporter PL C E E ? GTP GDP Neucler receptor DNA
Major pathway of cellular signal transduction GP~ TPK~ GC (guanylyl cyclase)~ Nuclear ~
(1) Signal transduction pathway introduced by GP receptor GP DG-PKC PLC β AC IP 3、Ca 2+-Ca. K
β-R α 2 -R,M-R α 1 -R,ET-R Cori, 1947 Gsα Gi Gqα ? AC PLC β + Gilman 1994 c. AMP PIP 2 IP 3 c. GMP? Sutherland 1971 PKA DAG(DG) Murad 1998 gene Pro* Krebs 1992 Edmond H. Fischer PKC glycogenolysis GPCR signal pathway
Mechanism of GP GTP GP(G ) = GTPase GDP off on GEF + GAP - Small GP (G ) guanine-nucleotide exchange factor GTPase activating protein
signal transduction pathway introduced by GP -R β-R α 2 -R,M-R α 1 -R,ET-R Gi Gsα + - AC c. AMP PKA Target Pro phospho Gqα + + PLCβ PIP 2 IP 3 DAG(DG) Ca 2+ released Targetgene transcription PKC Target Pro phospho
Cholera toxin, CTX Cl-、H 2 O GTP CTX leads to Gsαarg 201 ADPribosylation c. AMP GDP AC ATP
Pertussis toxin, PTX Giα PTX leads to Giα ADPribosylation, blocks its activation AC + PLCβ
(2) signal transduction pathway introduced by TPK • Receptor tyrosine protein kinase, RTK (20 types) • PTK-linking receptor
1)Receptor tyrosine protein kinase, RTK (20 types) TPK Ras-MAPK PLC -PIP 2 PI 3 K Proliferation differentiation
GF >50 kinds TPK PI 3 K Grb 2 Sos PKB Target pro phosphorylation MEK ERK PLC PIP 2 Ras DAG Raf PKC IP 3 Ca 2+ Transcriptional factor phosphorylation DNA
Receptor Tyrosine Kinases
2)PTK-linking receptor IL、IFN、erythropoietin(most cytokine) JAK FAK PTK in Src family PTK phosphorylation STAT inducing transcription regulating express gene DNA response element cellular phenotype change JAK-STAT Pathway
(3) Signal transduction pathway introduced by GC
Furchgott
cytokines Furchgott found CO Ca 2+ Ach-R GTP NO synthase arg R GC s. GC c. GMP PKG NO Vascular dilation ? VEC NO VSMC Vascular GC signal transduction system
(4) Signal transduction pathway introduced by nuclear receptor GC, Mineralo~, gonadal H; Steroid hormon-R in cytoplasma except estrogen R; bind to HSP T 3, Vit D, Tretinoin; Thyroxine hormon-R Dimer; in ? bind to pro or DNA ? -R as ligand-dependent transcription factor
Crosstalk one or more components of one signal transduction pathway affect another
3. Pathophysiology of CST Etiology and pathogenesis (1) Structure and expression change of gene (2) Abnormal function of immune (3) Secondary abnormality
(1) Structure and expression change of gene signal pro ( p 53 ) amount : ↓or↑ function : ↓ or↑ structure(mutation) : domain; deactivated; continually activated; dominant negative effect GF-GFR: acromegaly and gigantism
Hormone resistance syndrome A disease caused by target cell reducing or losing its response to the hormone, but the hormone synthesis and secretion in normal level Nephrogenic diabetes insipidus
Constitutive activation Receptor hyperactivation out of control due to gene mutation, also known as the receptor gaining functional mutation
(2) Abnormal function of immune Self-antibody against Signal Pro
v Stimulating Ab to the receptor for thyroid-stimulating hormone (TSH) Hyperthyroidism, proptosis (protrusion of the eyes globes), Graves’ disease v Blocking Ab to the TSHR v Hypothyroidism, myxedema Hashimoto’s thyroiditis
(3) Secondary abnormality • Blood p. H • Ion concentration ·Pulling on single molecules : Nature. . . • ATP
Receptor up-regulation or down-regulation Receptor hypersensitivity or desensitization
4. Abnormal signal transduction and disease One or multiple pathways One or multiple steps
(1) Insulin-resistant diabetes (type II) abnormal receptor, deficiency behind receptor Glucose -carry PTK insulin Glycogen thynthase Cellular proliferation
(2)Malignant tumor Biological features • hyperproliferation • hypodifferentiation • hypoapoptosis • metastasis
Cellular canceration • Proto-oncogene: over-expression, mutation • Tumor depressor gene: mutation, loss, lowexpression • DNA repair gene: mutation, loss, incorrect repair (polβ)
Cellular canceration total features multifactors , multisteps, multigenes Colon cancer as a model
From normal cell to cancer cell
Tumor suppress gene negative signal : keep cell in G 1 phase following specific program to differentiate to be senile to be apoptosis
(3) Autoimmune receptor disease 1) Ab against receptor: structure change ; same antigen 2) Ab against specificity: • Stimulating Ab + TSHR --- Graves disease • Blocking Ab + TSHR --- Hashimoto disease • Blocking Ab + n. Ach. R --- Myasthenia gravis chronic thyroiditis
(4) Inflammation • More cells, factors , complicated net LPS-R TNF-R IL-1 R
TNFa R sphingomyelinase SM SMase PK ceramide + NF- B P 65 P 50 Gene transcription NF- B I B Cytokine, IM I B
Immune in stress β 2 -adr-R β-arrestin 2 inhibit Activation of NF- B
(5) Cardiovascular disease Myocardial Remodeling Myocardial hypertrophy
Ang-II Mechanic stimuli R-TPK integrin Pro * nucleus Gene transcription Mechanism of VSMC or myocardial Hypertrophy
BODYBUILDING Mechanic pressure stimulation
5. Principles for Treatment ①To regulate ligands ②To regulate receptors ③To regulate intracellular messenger and transducers ④To regulate nuclear transcription factors
( end )
- Cell signal transduction
- Cellular communication pogil
- 3 stages of cell communication
- Essential cell biology chapter 3 quiz
- Signal transduction
- Signal transduction
- Which moon phase occurs directly before a new moon
- Which moon phase occurs directly before a new moon
- Moon sister moon calendar
- Home.hiwaay.net/ krcool/astro/moon/moon tides/
- How many days for a moon cycle
- Striate cortex
- Baroreceptors chemoreceptors mechanoreceptors
- Location aware computing
- Transduction in the ear
- Where does transduction occur in the ear
- Dv-2400 video rover
- Pitch in waves
- Sagittiform body shape
- Rover predator fish
- Generalized transduction
- Cajal bodies
- Tata swot
- Dad mom sister brother rover division
- Comedy of manners
- Transduction psychology
- Rover technology
- Hahna alexander
- Melos
- Rover ruckus game elements
- Cadillac escalade rental houston
- Sojourner mars rover
- Transduction psychology
- Olfactory transduction
- Olfactory transduction
- Olfactory transduction
- What are phytochromes
- Do subliminals work
- Olfactory transduction
- Transductant
- Rover.io
- Baseband signal and bandpass signal
- Baseband signal and bandpass signal
- Digital signal as a composite analog signal
- Even part of signal
- Unlv biomedical engineering
- Jennifer wissink cornell
- Guttman effect
- Zhao chun
- Zhao yuqi
- Irene zhao dao
- Jeremy zhao
- Ni zhao
- David zhao md
- Random sampling over joins revisited
- David zhao md
- Zhao qian malaysia