2016Impact Factor 2 95 Smart investigator Allergy Asthma

2016∙Impact Factor 2. 95 Smart investigator Allergy, Asthma & Immunology Research July 10, 2017 How to write a scientific paper-2 (Author) Hae-Sim Park MD, Ph. D Department of Allergy and Clinical Immunology Ajou University School of Medicine Suwon, South Korea e-aair. org

Process l On line submission format screening reviewers respond back to authors first/second/third? revisions final decision Decision and comment letter l General l Major comments concerns/ Minor concerns l English editing, reference and statistical methods 2

Before your submission, pl check title, author list and running title Suh DI et al. Allergy Asthma Immunol Res. 2017; 9: 200 -211. 3

Corresponding author information and COI included in the cover letter Suh DI et al. Allergy Asthma Immunol Res. 2017; 9: 200 -211. 4

Immunoblot/cell 사진은 해상도를 높여서 보내 주세요. Kim BG et al. Allergy Asthma Immunol Res. 2017; 9: 257 -264. 5

l Table 내 첫 자는 대문자 l 모든 수치에 단위 포함 l P value 는 소수 세자리까지 (P=0. 000 P<0. 001) Lee SJ et al. Allergy Asthma Immunol Res. 2017; 9: 212 -219. 통일

Discussion 과 references 사이 Suh DI et al. Allergy Asthma Immunol Res. 2017; 9: 200 -211. 7

정확성은 1저자 책임, 교신 저자의 확인 필요 REFERENCE checking 과정, AAIR 최근 2년 논문 포함 부탁 Suh DI et al. Allergy Asthma Immunol Res. 2017; 9: 200 -211. 8

답변서 준비하기 Comment 방안모색 의견 1. Please, use the standard names for non-steroidal anti-inflammatory drugs exacerbated 2. respiratory disease, as published in Allergy. 2011; 66: 818– 29. AERD를 Non-steroidal anti-inflammatory drugs (NSAIDs)-exacerbated respiratory disease (NERD)로 변경하기 　 2 Editors's 2 -1. Details on the method of LTE 4 measurement and its comments: validation should be provided (in supplementary materials if needed). 문의하기 　 3 Editors's comments: 2 -2. Please, consider to use uniform units (pg/m. L and pg/mg creatinine). 단위 변경하기 　 4 Reviewer 1 1. The study included large number of asthmatics with NSAIDs hypersensitivity confirmed by bronchial challenge however some parameters were assessed in smaller groups 2. (urine and serum LTE 4) and it is not clearly stated how many patients had other assessments done like total Ig. E, genetic studies, paranasal sinuses CT, methacholine challenge etc. 각각의 변수에 n수 삽입하기 있다면, guide주기, 왜 metabolite sample이 작은지 설명하기 　 5 Reviewer 1 3. Authors should justify the choice of 7 clinical criteria used for cluster analysis, moreover HLA-DPB 1*0301 is not a clinical criteria. 왜 HLA을 선택하게 되었는지 설명하기 　 6 Reviewer 1 4. According to method section ANOVA was applied for the LTE 4 분석을 비모수로 검증한 것 보여주기, data on LTE 4 measurements, however the number of patients 작은 수로 분석한 제한점 본문에 밝히기 in the clusters was low (n=7) and non-parametric tests should be used. Moreover it should be clearly stated in method section that measurements of urine and serum LTE 4 were assessed only in small percentage of patients from each cluster. 7 Reviewer 1 5. The discussion should be re-written as it neglects the results of the only one cluster analysis of Bochenek et al (JACI 2014) and do not contrast the results of present study with results of Bochenek No From 1 Editors's comments: 8 Reviewer 1 5. There is no discussion of potential limitation of the study. 　 Discussion에서 2014 jaci 논문과 대비하면 기술하지 말 　 아라, 이부분 재작성 제한점 기술하기: Reallife study로 환자의 정보를 역 　 으로 추적하여, 그 특성을 분리하였다. 허나, 이 정보 가 실제 prospective 연구에서도 적합하다고 확인하는 단계가 필요하다. 추가 교수님 의견 받기

Response letter Thank you letter, Speedy, Describe in detail to each comment, English editing if needed Dear Editors and Reviewers, We are greatly thankful to your careful reviews and helpful comments to our manuscript. We have revised and amended the manuscript according to your suggestions and our responses to each comment are provided below. We hope that our revised manuscript is suitable for publication in the AAIR. General comments: 본인의 의견 추가 가능 Reviewer 1 This study investigated autophagy and NET production levels by neutrophils isolated from peripheral blood samples taken from patients with severe asthma or with less severe disease. The effects of NET on in vitro inflammatory functions were also examined in human airway epithelial cells or peripheral blood eosinophils. Overall this is a potentially interesting study but I have a number of queries. 1. My main concern is that from Table 1 30 SA patients and 38 NSA patients were recruited yet in Figure 1 B data from 24 and 22 patients and for 1 D 14 and 8 respectively are presented. The same issue applies to Figure 2 and 3. Was this simply due to lack of cells or was any selection made. If the latter is the case how was this done? à Thank you for your comment. We described the study cohort in the “Materials and Methods” section (Lines 79 -85) and “Results” section (Lines 223 & 232). , We recruited totally 68 asthmatic patients into 2 independent cohorts of with SA (n=30) and NSA (n =38) patients. In the first cohort, we investigated autophagy levels in PBNs isolated from 22 SA and 24 NSA patients using immunoblots. Because of limitations in number of PBNs that could be obtained from each study subject, we further recruited 8 SA and 14 NSA patients to the second cohort to evaluate neutrophil autophagy and NET production levels using confocal microscopy and Pico. Green assay. No special selection was made to divide the subjects into the 2 cohorts. à Reference, 관련 table and figure 추가 가능(list)

2016∙Impact Factor 2. 95 p. ISSN 2092 -7355 e. ISSN 2092 -7363 in SCIe Web of Science® Pl cite the recent AAIR articles published during 2016 -2017 e-aair. org The Korean Academy of Asthma, Allergy and Clinical Immunology The Korean Academy of Pediatric Allergy and Respiratory Disease