Timing KORAY TEKIN Ankara niversitesi Veteriner Fakltesi Reprodksiyon

Timing ? KORAY TEKIN Ankara Üniversitesi, Veteriner Fakültesi Reprodüksiyon ve Suni Tohumlama Anabilim Dalı

Fertile period Fertilisation period is the time that oocytes are fertilisable within the female reproductive tract • Mating trigger ovulation, • Mature secondary oocyte is ovulated and this may survive for two to four days after ovulation • Fertilisation period two to five days after mating; • İncreasing progesterone concentrations close the cervix. • Sperm must introduced at mating • For AI, introduce sperm within days 3 to 4 after injection of drugs or mechanical stimulation of the vagina to cause induction of ovulation. Gary C. W. England, and Marco Russo In Practice 2014; 36: 249 -254

Determining time for insemination • Queens spontaneously cycling, but not ovulating, • Return to pro-oestrus approximately every three weeks. • Ovulation induced by mechanical stimulation or Gn. RH (25 µ/queen), h. CG (100 iu/queen) on day 2 or 3 of oestrus. • AI is best performed on days 3 and 4 after the stimulation

Artificial insemination technique • Ejaculate is deposited into the cranial vagina • Interestingly, semen flow into the uterus in dorsal recumbency and the table tilted 30 degrees. • Transcervical uterine insemination in the queen is problematic «vagina is narrow» • Furthermore, the cervix is positioned on the dorsal wall of the vagina and the cervix is angled craniodorsally, making directing the catheter through the cervical os technically difficult.

Number of inseminations • A single insemination on day 3 or 4 (ie, on day 2 or 3 after ovulation). • Sedate with alpha 2 adrenoreceptor agonist • Two inseminations should be performed on days 2 and 3 after ovulation when cryopreserved semen is used.