Simple Lectin Identification Using a Chemical Tongue Lucienne

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Simple Lectin Identification Using a ‘Chemical Tongue’ Lucienne Otten Supervised by: Dr. Matthew Gibson

Simple Lectin Identification Using a ‘Chemical Tongue’ Lucienne Otten Supervised by: Dr. Matthew Gibson

Antibiotic resistance % of S. aureus individuals carrying resistant strains No. of cases of

Antibiotic resistance % of S. aureus individuals carrying resistant strains No. of cases of MDR-TB in thousands CDC

Protein-Carbohydrate Interactions Holgersson et al. Immunol Cell Biol (20

Protein-Carbohydrate Interactions Holgersson et al. Immunol Cell Biol (20

Point of care diagnostics CFG database

Point of care diagnostics CFG database

Mono-/di-saccharides CFG database

Mono-/di-saccharides CFG database

Oligosaccharides CFG database

Oligosaccharides CFG database

Surface Functionalisation Godula, K. and Bertozzi, C. R. J. Am. Chem. So

Surface Functionalisation Godula, K. and Bertozzi, C. R. J. Am. Chem. So

Binding profiles

Binding profiles

Linear Discriminant Analysis

Linear Discriminant Analysis

Linear Discriminant 2 Linear Discriminant 1

Linear Discriminant 2 Linear Discriminant 1

Binding profiles Otten, L and Gibson, MI. Manuscript in

Binding profiles Otten, L and Gibson, MI. Manuscript in

Linear Discriminant Analysis Linear Discriminant 2 DBA PNA RCA 120 SBA Linear Discriminant 1

Linear Discriminant Analysis Linear Discriminant 2 DBA PNA RCA 120 SBA Linear Discriminant 1 Otten, L and Gibson, MI. Manuscript in

Identification of blind samples Otten, L and Gibson, MI. Manuscript in

Identification of blind samples Otten, L and Gibson, MI. Manuscript in

Linear Discriminant 2 Identification of blind samples Sample Predicted U 4 Actual U 1

Linear Discriminant 2 Identification of blind samples Sample Predicted U 4 Actual U 1 RCA 120 U 2 PNA U 3 SBA U 4 DBA U 1 U 3 U 2 Linear Discriminant 1 Otten, L and Gibson, MI. Manuscript in

Bacterial labelling R= biotin

Bacterial labelling R= biotin

Bacterial labelling

Bacterial labelling

Bacterial LDA model Top 10 M. smegmatis K 12

Bacterial LDA model Top 10 M. smegmatis K 12

LDA Predictions Sample Predicted Actual U 1 M. smegmatis U 2 M. smegmatis U

LDA Predictions Sample Predicted Actual U 1 M. smegmatis U 2 M. smegmatis U 3 M. smegmatis U 4 M. smegmatis U 5 Top 10 U 6 Top 10 U 7 Top 10 U 8 Top 10 U 9 K 12 U 10 K 12 U 11 K 12 U 12 K 12

CFG data Fim. H Fli. D Typhoid toxin CFG database

CFG data Fim. H Fli. D Typhoid toxin CFG database

So why not use more sugars? Correct prediction (%) 80 70 60 50 40

So why not use more sugars? Correct prediction (%) 80 70 60 50 40 30 20 10 0 3 4 5 6 7 8 9 10 11 Number of monosaccharides 12 13 14 α-glucose, α-galactosamine, α-mannose, fucose, β-galactose, β-glucose and βgalactosamine CFG database

Conclusions • Linear discriminant analysis has been shown to aid in the identification of

Conclusions • Linear discriminant analysis has been shown to aid in the identification of blind lectin samples • It has also shown to be applicable to identification of bacterial species • Analysis of larger data sets highlighted the need for identifying ‘optimum surfaces’.

Future work • Extend the bacterial LDA analysis to more species of bacteria •

Future work • Extend the bacterial LDA analysis to more species of bacteria • Extend the LDA analysis of the CFG database to beyond monosaccharides.

Acknowledgements Gibson Group Members - Gemma-Louise Davies Yunhua Chen Catherine Cooper Robert Deller -

Acknowledgements Gibson Group Members - Gemma-Louise Davies Yunhua Chen Catherine Cooper Robert Deller - - Caroline Biggs Daniel Phillips Tom Congdon Sarah-Jane Richards - Sang-Ho Won - Laura Wilkins - Robert Keogh Daniel Mitchell Richard Lowery Ben Martyn Lewis Blackman Matthew Gibson

Lectin leftovers 22707 Lectin leftovers Brief review ofLectin leftovers 22707 Lectin leftovers Brief review of
The cartilagespecific lectin Ctype lectin domain family 3The cartilagespecific lectin Ctype lectin domain family 3
HISTOLOGY OF TONGUE TONGUE HISTOLOGY Defn Tongue isHISTOLOGY OF TONGUE TONGUE HISTOLOGY Defn Tongue is
TONGUE ANKYLOGLOSSIATONGUE TIE GEOGRAPHIS TONGUE BLACK HAIRY TONGUETONGUE ANKYLOGLOSSIATONGUE TIE GEOGRAPHIS TONGUE BLACK HAIRY TONGUE
Powerpoint Activity 1 Tongue Twister Practice Tongue TwistersPowerpoint Activity 1 Tongue Twister Practice Tongue Twisters
Tongue The tongue is fleshy movable muscular organTongue The tongue is fleshy movable muscular organ
TONGUE MUSCLES INNERVATIONS INTRODUCTION Tongue is a muscularTONGUE MUSCLES INNERVATIONS INTRODUCTION Tongue is a muscular
The Tongue James 3 1 12 The TongueThe Tongue James 3 1 12 The Tongue
Please keep separate Tongue roll No tongue rollPlease keep separate Tongue roll No tongue roll
FROM THE HEART TO THE TONGUE Tongue aFROM THE HEART TO THE TONGUE Tongue a
INNERVATION OF THE TONGUE NERVE SUPPLY OF TONGUEINNERVATION OF THE TONGUE NERVE SUPPLY OF TONGUE
TONGUE MUSCLES INNERVATIONS INTRODUCTION Tongue is a muscularTONGUE MUSCLES INNERVATIONS INTRODUCTION Tongue is a muscular
The Tongue taste The tongue is the organThe Tongue taste The tongue is the organ
Tongue Tongue Mass of striated muscles covered withTongue Tongue Mass of striated muscles covered with
Taming the Tongue Taming the Tongue Who isTaming the Tongue Taming the Tongue Who is
SIMPLE SUBJECT SIMPLE PREDICATE SIMPLE SUBJECT A simpleSIMPLE SUBJECT SIMPLE PREDICATE SIMPLE SUBJECT A simple
Present Simple Past Simple Future Simple PRESENT SIMPLEPresent Simple Past Simple Future Simple PRESENT SIMPLE
Simple Present Simple Past Simple Future Simple PresentSimple Present Simple Past Simple Future Simple Present
Simple Machines Simple Machines Simple machines are simpleSimple Machines Simple Machines Simple machines are simple
PAST SIMPLE PRESENT SIMPLE FUTURE SIMPLE Past SimplePAST SIMPLE PRESENT SIMPLE FUTURE SIMPLE Past Simple
Simple Chemical Reactions Contents 7 F Simple ChemicalSimple Chemical Reactions Contents 7 F Simple Chemical
Simple Chemical Reactions Contents 7 F Simple ChemicalSimple Chemical Reactions Contents 7 F Simple Chemical