l ACL 9000 l ACL 9000 ACL Futura

목적 l ACL 9000 장비 소개 l ACL 9000 과 ACL Futura plus 장비간 비교

현재 사용중인 혈액응고 검사장비 - ACL Futura plus(IL, Italy) 2대 ACL 3000(IL, Italy) 1대 : PT, APTT, Factor, LAC - STA Compact(STAGO, France)1대 : Antithrombin III, Thrombin Time Protein C, Fibrinogen

ACL 9000 주요특징 True random access § Nephelometric method & Chromogenic method § Stat 검사 가능 § Rotor 전자동 이송장치 § LCD touch screen §

ACL 9000 - 측정원리 1) Nephelometric method -광원: LED, 660 nm(정밀한 광도유지) -90° Light scattering 측정 -1, 100회/1분 측정 -검사항목 : PT-Fib, PTT, 외인계/내인계Factor, LAC, Protein C/S

ACL – 9000 측정원리 2) Chromogenic/Immunological method -광원: Halogen lamp, 405 nm -180° Absorbance측정 -1, 100회/1분 측정 -검사항목 : Antithrombin, Plasminogen Plasmin inhibitor, Heparin. Xa, Protein C/S, v. WF Ag/Activity, D-dimer

ACL 9000 - Reagent Area(1) 8개의 시약 장착가능 -냉장, stirring : 4 position -실온 : 4 position § Original Vials (4, 10, 16 ml) §

ACL 9000 - Sample Tray Area(2) 40 검체 장착가능 (Original tube, Cups사용) § 10개의 부속시약 위치 § Bar-code Reader내장 §

ACL 9000 - Fluidics(3) 1 liter Wash-Reference bottle 사용 (잔여 액위 감지 기능) § 2개의 Piston diluter(Sample/Reagent) §

ACL 9000 - Rotor Exchange(4) l Rotor arm을 통한 전자동 이송

ACL 9000 – Throughput § 처리속도(1검체) - PT/Fib : 4 min 50 sec - APTT : 6 min 50 sec - PT/Fib/APTT : 8 min 30 sec § 처리속도(시간당) - PT/Fib : 175검체 - APTT : 110검체 - PT/Fib/APTT : 60검체

응고 장비별 특징 비교 기종 종류 Futura Plus ACL 9000 ACL 3000 검사원리 Turbidimetric & Chromogenic Random Access ○ ○ X 처리속도 PT-Fib (test/1 hr) PTT 240 175 180 110 최대 검체 장착수 120 검체 가능 40검체 가능 18검체 불가능 Stat 검사 Nephelometric & Chormogenic Chromogenic

Correlation Test (ACL 9000 & Futura Plus) § 대상: 무작위 50검체 (정상/비정상 고르게 분포) § 종목: PT, PTT, Fibrinogen § 실시: 2005. 04

PT Correlation Curve(Sec)

PT Correlation Curve(INR)

Fibrinogen Correlation Curve (mg/dl)

APTT Correlation Curve(Sec)

Accordance (ACL 9000 & Futura Plus) l INR, APTT, Fibrinogen: good accordance (paired t-test, Wilcoxon signed rank test) l PT: <2% difference (mean ratio 0. 986)

Precision test (within run) § 방법 : Normal(Homemade) Low abnormal control High abnormal control - 각각 24회 test § 종목 : PT, PTT, Fibrinogen

Precision test - Result PT(sec) 　 Normal sec INR APTT(sec) FBG(mg/ d. L) Mean 11. 2 0. 869 29. 2 319. 9 SD 0. 1 0. 011 0. 4 11. 2 C. V. 0. 9 1. 3 1. 4 3. 5 1. 68 7. 06 C. V. Acceptable value 1. 30 Mean 23. 1 2. 5 54. 5 123. 7 SD 0. 5 0. 1 0. 5 5. 1 C. V. 2. 0 2. 9 0. 9 4. 1 1. 72 7. 14 Low Abnormal - C. V. Acceptable value 2. 07 Mean 32. 1 4. 1 67. 1 - SD 0. 5 0. 1 0. 7 - C. V. 1. 6 2. 3 1. 1 - 3. 02 - High Abnormal C. V. Acceptable value 2. 07 - -

결론 l Satisfactory correlation l Satisfactory accordance – INR, APTT, Fibrinogen – PT<2% l Satisfactory precision