1 RNA structure 2 RNA structure RNA can
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1 RNA structure
2 RNA structure RNA can be described according to the primary sequence (A), a secondary structure (B) which may contain various structural elements, a super-secondary structure (C) which may contain pseudoknots another tertiary interactions, and a three-dimensional structure (D) wich may interact with proteins (green). Bevilacqua, P. C. , Ritchey, L. E. , Su, Z. & Assmann, S. M. Genome-Wide Analysis of RNA Secondary Structure. Annu Rev Genet 50, 235 -266, doi: 10. 1146/annurev-genet-120215 -035034 (2016).
3 in vivo RNA structure is often predicted by computer algorithms In silico These algorithms can be modified to include restraints or constraints on the structure (places where you know are unpaired or base-paired) Often different structures are predicted if restraints are included (bottom) or excluded (top) In vivo Bevilacqua, P. C. , Ritchey, L. E. , Su, Z. & Assmann, S. M. Genome-Wide Analysis of RNA Secondary Structure. Annu Rev Genet 50, 235 -266, doi: 10. 1146/annurev-genet-120215 -035034 (2016).
4 Genome-wide processes RNA structure and function are often interrelated These relationships are best understood on a genomic scale. Bevilacqua, P. C. , Ritchey, L. E. , Su, Z. & Assmann, S. M. Genome-Wide Analysis of RNA Secondary Structure. Annu Rev Genet 50, 235 -266, doi: 10. 1146/annurev-genet-120215 -035034 (2016).
T G C A Structural determination 5 5’ 5’ 32 P - +
6 New methodology
