HPLC Phytoplankton Pigment Measurements Continuation of Climate Quality

HPLC Phytoplankton Pigment Measurements: Continuation of Climate Quality Data Records (CQDR) in Support of NASA’s Earth Science Enterprise (ESE) C. C. Trees and J. L. Mueller Center for Hydro-Optics and Remote Sensing (CHORS) San Diego State University (ctrees@chors. sdsu. edu) CHORS, SDSU MODIS Science Team Meeting (BWI, MD, 22 -24 Mar 05)

HPLC Phytoplankton Pigment Protocols (Bidigare et al. , 2003; NASA/TM-2003 -211621/Rev 5 - Vol. V) MODIS Data Products Program ~ 4, 800 samples/yr Method Comparison Co-elution of mono- and divinyl Chl a 1. Wright et al. (1991) C 18 Method (SIMBIOS) Channels ratio (436 & 450 nm) Physical separation of mono- and divinyl chl a 1. Goericke and Repeta (1993) C 8 Method 2. **Van Heukelem and Thomas (2001) C 8 with column heater Method CHORS, SDSU MODIS Science Team Meeting (BWI, MD, 22 -24 Mar 05)

Divinyl Chl b Divinyl & Monovinyl Chl a (664 nm) CHORS, SDSU MODIS Science Team Meeting (BWI, MD, 22 -24 Mar 05) Monovinyl Chl a Divinyl and Monovinyl Chl a Separation

CHORS, SDSU Fucoxanthin Chl c 3 MODIS Science Team Meeting (BWI, MD, 22 -24 Mar 05) b. e carotene b. b carotene Monovinyl Chl a Culture Mixture (450 nm) Monovinyl Chls a’, a” Monovinyl Chl b Zeaxanthin Alloxanthin Diatoxanthin Diadinoxanthin Prasinoxanthin 19’-Hexanoyloxyfucoxanthin Peridinin Chl c 2 Injection Spike Mixture of Pigments from Cultures

HPLC Samples Processed/Submitted CHORS, SDSU MODIS Science Team Meeting (BWI, MD, 22 -24 Mar 05)

CHORS Pigment Database Total Accessory = 1. 082 Total Chl a 0. 906 R 2 = 0. 956 n = 20, 297 CHORS, SDSU MODIS Science Team Meeting (BWI, MD, 22 -24 Mar 05)