Who Ate the Cheese Forensic Science 12814 S
- Slides: 8
Who Ate the Cheese? Forensic Science 1/28/14 S
Drill S What does RFLP stand for? S What are the enzymes that cut DNA called? S How many bands do you expect for each RFLP tested? Why? S HW: Finish RFLP tests
Objectives S IWBAT S Analyze DNA to determine the most likely suspect. S Analyze a RFLP test.
Agenda S Drill S Who Ate the Cheese? S RFLP Practice S HW Review
Who Ate the Cheese? S Work in pairs. Each pair will get: S A description of the case scenario S DNA Evidence Evaluation sheet (you will cut this up) S A sheet of printer or graph paper S A roll of tape
Steps to Who Ate the Cheese? S Read the scenario and the directions. S One partner will prepare the “agarose gel” S One partner will prepare the “samples” (scissors) S Create your finished “gel” and answer the questions on the back of the scenario paper. Use the textbook, if needed. S Staple the “gel” and questions together. Turn in the final product.
Who Ate The Cheese? S After cutting out your fragments, count how many base pairs there are in each fragment – Ms. Bloedorn will demonstrate. You may color-code the fragments, if you like (ex. Suspect 1 = blue, etc. ) S Lay it out horizontally, as Ms. Bloedorn demonstrates. Your range is 0 -31 bps. S You may end up with fragments overlapping on “lanes”. Just fold the fragments over before taping them down.
RFLP Analysis S Get the RFLP Analysis worksheet. Work until everyone finished “Who Ate the Cheese? ” S Let’s review the HW, so far! Ch 13 Rev Qs 1 -23
