Supplementary figure S 1 A Survival Entering a

Supplementary figure S 1 (A) Survival Entering a cage Day 1 2 -DG (or water) Standard diet High-fat diet 5 (B) 6 7 Day 1 Day 8 (Sampling) Entering a cage (weeks of age) 2 -DG (or water) Standard diet High-fat diet 5 6 7 (weeks of age) Experimental protocols to examine the survival rate (A) and obtain the physical parameters and blood samples (B). 2 -DG: 2 -deoxyglucose

Supplementary figure S 2 A B C D E F Standard diet HFD Effects of 2 -DG on b-hydroxybutyric acid (A), lactate (B), base excess (C), p. H (D), hematocrit (E) and potassium ion (F) in the 4 groups of mice. Blood samples were obtained by cardiac puncture under anesthesia at day 8 (n=7 in all groups). HFD: high-fat diet; 2 -DG: 2 -deoxyglucose. Statistical analysis was performed by 2 -way ANOVA followed by Bonferroni’s post hoc test. *p<0. 05, **p<0. 01; ***p<0. 001

Supplementary figure S 3 A B Effects of 2 -DG on glucose (A), and total cholesterol (B) levels in the 4 groups of mice. Blood samples were obtained by cardiac puncture in a fed state under anesthesia at day 8 (n=9 in all groups). A, Control (water) group with standard diet and high fat diet (HFD); 2 -deoxyglucose (2 DG) group with standard diet and HFD. B, Control (water) group with standard diet and HFD; 2 -DG group with standard diet and HFD. Statistical analysis was performed by 2 -way ANOVA followed by Bonferroni’s post hoc test. *p<0. 05, ***p<0. 001