Supplementary Figures Supplementary Figure 1 AD Principal coordinate

  • Slides: 9
Download presentation
Supplementary Figures

Supplementary Figures

Supplementary Figure 1 A-D. Principal coordinate analysis (PCo. A) plots comparing microbiota composition based

Supplementary Figure 1 A-D. Principal coordinate analysis (PCo. A) plots comparing microbiota composition based on maternal BMI, glucose tolerance status, mode of delivery and ethnicity (weighted Uni. Frac distance metric). (A) (C) (B) (D)

Figure S 1 -E (E)

Figure S 1 -E (E)

Supplementary Figure 2 A-D. Principal coordinate analysis (PCo. A) plots comparing microbiota composition based

Supplementary Figure 2 A-D. Principal coordinate analysis (PCo. A) plots comparing microbiota composition based on maternal BMI, glucose tolerance status, mode of delivery and ethnicity Bray-Curtis dissimilarity metric). (A) (B) (C) (D)

Figure S 2 -E (E)

Figure S 2 -E (E)

Supplementary Figure 3. Relative abundance of top 20 KEGG pathways across all mother’s milk

Supplementary Figure 3. Relative abundance of top 20 KEGG pathways across all mother’s milk samples.

Supplementary Figure 4. Significant association observed between KEGG pathway ‘Biosynthesis of secondary metabolites’ and

Supplementary Figure 4. Significant association observed between KEGG pathway ‘Biosynthesis of secondary metabolites’ and BMI (obese sub-category)

Supplementary Figure 5. Principal coordinate analysis (PCo. A) plots (A) Weighted Uni. Frac distances

Supplementary Figure 5. Principal coordinate analysis (PCo. A) plots (A) Weighted Uni. Frac distances comparing microbiota composition based on negative controls and milk samples (B) Bray Curtis dissimilarity comparing microbiota composition based on negative controls and milk samples (A) (B) R 2= 0. 07, p-value= 0. 0001 R 2= 0. 10, p-value= 0. 0001

Supplementary Figure 6. Consort Statement. Flow diagram of subject participation at each time point.

Supplementary Figure 6. Consort Statement. Flow diagram of subject participation at each time point. Potential pregnancies followed annually at Mount Sinai hospital (n= >7, 000) Baseline visit completed (n= 216) Women who donated at least one milk sample (n= 170) Women who did not or could not provide a milk sample (n= 46) Women who where unable to donate a mature milk sample at 3 months post-partum (n= 35) Women who did not donate due to breastfeeding discontinuation (n= 14) Women who declined to provide a sample but continued breastfeeding (n= 4) Women who provided mature milk sample at 3 months post-partum (n= 117) Milk samples with inadequate volume (n= 4) Samples remaining for microbiota analyses (n= 113) Samples removed during rarefying and filtering (n= 4) Samples remaining after filtering and rarefying data (n= 109)