NonLactose Fermentors Salmonella Shigella Proteus Salmonella 1 Salmonella

Non-Lactose Fermentors Salmonella Shigella Proteus

Salmonella

1. Salmonella a. Growth of Salmonella on Mac. Conkey agar: Non-lactose fermenter (colonies pale colorless) b. Growth of Salmonella on SS agar: Non-lactose fermenter with black center due to the production of H 2 S c. Biochemical tests of Salmonella: • Indole: negative • Citrate: positive • TSI: K/A, Gv H 2 S+ • Motility test: motile • Urease test: negative d. Serology test for Salmonella infection : Widal test

Shigella

2. Shigella a. Growth of Shigella on Mac. Conkey agar: Non-lactose fermenter (colonies are pale colorless) b. Growth of Shigella on SS agar: Non-lactose fermenter c. Biochemical tests of Shigella: • Indole: variable • Citrate: negative • TSI: K/A, G- H 2 S • Motility test: non-motile • Urease test: negative

Shigella Salmonella - Motility + Glucose AG (except S. typhi) A Indole + H 2 S + -

3. Proteus a. Growth of Proteus on Mac. Conkey agar: Non-lactose Ø fermenter (colonies are pale colorless) b. Growth of Proteus on blood agar: observe the Ø swarming and smell the fishy odor c. Biochemical tests for Proteus: Ø • Indole: variable Ø • Citrate: variable Ø • TSI: K/A, G- H 2 S+ Ø • Motility test: motile Ø • Urease test: positive Ø • Phenylalanine deaminase test: positive Ø