ZILOStk November 2005 Hamilton Thorne Biosciences ZILOStk Zona

ZILOS-tk- November 2005 Hamilton Thorne Biosciences ZILOS-tk Zona Infrared Laser Optical System Version 5. 2

ZILOS-tk- November 2005 Introducing the ZILOS-tk § Integrated into 50 x objective – compact and portable § Installs on all current & older models of inverted microscopes § Turret rotates easily to use other objectives § Leaves both the fluorescent and filter cube ports open § Compatible with Spindleview® and all micromanipulators 2

ZILOS-tk- November 2005 Applications of ZILOS-tk § Laser Assisted Hatching* § Embryo Biopsy for PGD § ICSI § Polar Body Biopsy * Only cleared indication in US 3

ZILOS-tk- November 2005 Precision and Control of the ZILOS-tk § “The zona effect that is reached is precise, consistent, and controllable… inadvertent use is virtually impossible. ” Josiane Van der Elst, Ph. D. , Infertility Centre, Ghent University Hospital § “The ZILOS-tk system has proven itself to me and my colleagues time and again as the easiest and most effective tool for breaching the zona pellucida. Long-term experience within our labs has shown clearly that it is not only fast and user-friendly, but also "embryofriendly. ” Michael Tucker, Ph. D. , Scientific Director, Georgia Reproductive Specialists and IVF Lab Director, Shady Grove Fertility RCS § “The precision of the ZILOS-tk allowed Sydney IVF to move to day 5 trophectoderm biopsy, as the routine biopsy procedure. We are the only laboratory in the world able to make this claim. Kylie de Boer, Ph. D. , PGD Director, Deputy Director of Embryology, Sydney IVF 4

ZILOS-tk- November 2005 Take a Closer Look § 1480 nm, Class 1 Laser § Specially designed 50 x objective – functions in both infrared and visible wavelengths § Completely closed system for user safety § Power @ focus = 300 m. W 5

ZILOS-tk- November 2005 Easy Installation § Install turret adapter with hex key § Attach ZILOS-tk to adapter § Tighten lock nut § Connect controller 6

ZILOS-tk- November 2005 No Laser Alignment Needed § Laser aligned at factory and LOCKED in place § Allows easy transfer between microscopes § Simply align target to laser and you are ready to go 7

ZILOS-tk- November 2005 Software Interface 8

ZILOS-tk- November 2005 Overview of ZILOS-tk Software Features § § § § § Choice of laser modes Unlimited image capture with thumbnail preview Auto-capture on laser fire; Capture overlay Three target types: Isotherm Rings, Circle, or Arrow Real-time video capture Measurement utility Calibrate and store multiple objectives Digital zoom / pan Built-in patient reports 9

ZILOS-tk- November 2005 Simple to Use § User-friendly interface for quick learning § Easy to understand setup screens § All actions accessed from control panel • • No need to continuously open menus No need to remember shortcut keys 10

ZILOS-tk- November 2005 Clinical Mode § Three preset power and pulse combinations • • • Low Medium High § Automatic laser shutoff timer for safety § Choice of arrow, circle or Isotherm targets 11

ZILOS-tk- November 2005 Validation Mode § Adjustable pulse duration (1 µsec – 3000 µsec) § Adjustable power range (0 – 100%) Validation Mode not for use with human embryos 12

ZILOS-tk- November 2005 About the Isotherm Rings (Patent Pending) § Indicate the peak temperature down to 50°C (purple) § Show approximate drill hole size (orange) § Interactive – Isotherm Ring sizes change based on pulse duration § Select which rings to view 13

ZILOS-tk- November 2005 Bovine Oocyte: Drill Hole Sizes Power: 300 m. W Duration: Variable 1200 µs 1100 µs 1000 µs 200 µs 900 µs 300 µs 400 µs 700 µs 500 µs 600 µs 100 µm 700 µs

ZILOS-tk- November 2005 Thumbnail Images § Capture up to 15 thumbnail images § One-click “Save All” and “Delete All” § Print individual images or entire thumbnail gallery § Objective calibration stored with image, allowing accurate measuring upon recall 15

ZILOS-tk- November 2005 Video Recording § Record up to 1000 seconds continuous video § Set maximum record time to save disk space § One-touch start record / stop record § Automtic or manual file naming options § Recall and play video within ZILOS-tk 16

ZILOS-tk- November 2005 Measurement Utility § Store 5 separate sets of measurements § Automatically calculates mean and standard deviation § Transferred to report at touch of a button § Dimensions shown on image; may be saved with measurements 17

ZILOS-tk- November 2005 Built-in Data Management § Storage of individual images § Integrated report generation § Add pre- and post-treatment images to report § Choose 2 or 4 image format § Export data to ASCII (. txt and. mer) § Compatible with IDS, Baby. Sentry and Recdate 18

ZILOS-tk- November 2005 What You Get § ZILOS-tk laser - 50 x objective combination and laser control module § Three turret adapters § CCD camera with c-mount adapter § Full function software § High speed PC system – desktop or laptop 19

ZILOS-tk- November 2005 The Results Speak for Themselves § 270% increase in successful FET rates (frozen embryo transfer), from 15% to 41% (under 38 age group) Harley Street Fertility Centre § Pregnancy rate of 35% achieved in most difficult group (40+ and repeat failures) Assisted Conception Unit, Glasgow Nuffield Hospital 20

ZILOS-tk- November 2005 Hamilton Thorne Contact Information § Phone: 978 -921 -2050, 800 -323 -0503 § Fax: 978 -921 -0250 § E-mail: info@hamiltonthorne. com § Web: www. hamiltonthorne. com § 100 Cummings Center, Suite 465 E Beverly, MA 01915 USA 21