Zero Trans Start Hot Start Technology DNA Binding
비특이적 증폭 ‘Zero’에 도전 Trans. Start ® Hot Start Technology DNA Binding Protein l Antibody와 chemical 방식 보다 높은 특이성 ü Primer Blocking l No Primer dimer (기존 방식과의 차이점) ü Template Blocking l No Mammalian DNA 오염 (Antibody 방식의 단점) ü Polymerase Blocking l No long denaturing step (Chemical 방식의 단점) Double & Triple Blocking by DNA Binding Protein Primers Polymerase ü Binding protein이 primer에 결합 ü Non-specific binding과 extension 방지 Binding Protein ü초기 변성 단계에서 Binding protein 파괴 ü Primer가 template에 결합 ü PCR 반응 진행 Template Polymerase Taq Polymerase Efficiency & Specificity Fidelity (vs Easy. Taq®) Extension Rate Hot Start 3'-A tail Product Size (Human g. DNA) GC-rich & complex template Master Mix type Others High Fidelity (vs Easy. Taq®) Extension Rate Hot Start 3'-A tail ü Binding protein과 template 결합 ü Polymerase의 template binding 방해 Trans. Fast® Taq Easy. Taq® Trans. Taq® -T Trans. Taq® Hi. Fi Trans. Start® Taq Trans. Start® Top Taq + 1 x 6 kb/min + 1 x 1~2 kb/min ++ 18 x 1~2 kb/min +++++ 18 x 1~ 2 kb/min O (Triple) O ≤ 15 kb ++ X Easy. Pfu Trans. Start® Fast. Pfu Fly 18 x 54 x 108 x 0. 5 kb/min 2~4 kb/min 2~6 kb/min X O (Double) ++++ 18 x 1~2 kb/min O (Double) △ (Genetically modified Hot start) O O O ≤ 4 kb ≤ 8 kb ≤ 15 kb + + ++ + X O O O X Single Taq + pfu (Enzyme Mixture) X X X Product Size (Human g. DNA) ≤ 6 kb ≤ 15 kb Product Size (Plasmid DNA) ≤ 10 kb ≤ 20 kb O O X Master Mix type
DNA Binding Protein & Hot Start PCR DNA Polymerase Series ● 일정한 결과 ● 고품질 ● 편안한 가격 u 세계적 유명 메이커에 완제품 형태로 납품 하는 브랜드로 u 대부분의 제품이 유명 메이커의 엄격한 품질 기준에 부합 v DNA Polymerase Product Name Code Size Price Note Easy. Taq® DNA Polymerase AP 111 -11 500 U 50, 000 2×Easy. Taq® PCR Super. Mix AS 111 -02 5× 1 ml 157, 000 Trans. Taq®-T DNA Polymerase AP 122 -11 250 U 70, 000 2×Trans. Taq®-T PCR Super. Mix AS 122 -02 5× 1 ml 294, 000 Trans. Taq® DNA Polymerase Hi. Fi AP 131 -11 250 U 90, 000 2×Trans. Taq® Hi. Fi PCR Super. Mix I AS 131 -02 5× 1 ml 307, 000 l For Genomic DNA 2×Trans. Taq® Hi. Fi PCR Super. Mix II AS 131 -22 5× 1 ml 307, 000 l For plasmid, λDNA, c. DNA Trans. Start® Taq DNA Polymerase AP 141 -11 250 U 116, 000 l Blended Taq (Taq + pfu) l Double Blocking Hot start Trans. Start® Top. Taq DNA Polymerase AP 151 -11 250 U 141, 000 l Blended Taq (Taq upgrade + pfu) l Triple Blocking Hot start Easy. Pfu DNA Polymerase AP 211 -11 250 U 64, 000 2×Easy. Pfu PCR Super. Mix AS 211 -02 5× 1 ml 294, 000 Trans. Start® Fast. Pfu DNA Polymerase AP 221 -11 250 U 173, 000 2×Trans. Start® AS 221 -02 5× 1 ml 545, 000 AP 231 -11 250 U 231, 000 l General Taq (~ 4 kb) l Blended Taq (Taq + pfu) l Long & Complex (Taq-T + pfu plus) l High fidelity Fast. Pfu PCR Super. Mix Trans. Start® Fast. Pfu Fly DNA Polymerase l Fast High fidelity (2 ~ 4 kb/min) l Double Blocking Hot start l Fast Higher fidelity (2 ~ 6 kb/min) l Double Blocking Hot start v SYBR Green q. PCR Super. Mix Product Name Code Size Trans. Start® Top Green q. PCR Super. Mix AQ 131 -02 5× 1 ml 200, 000 l Double Blocking (Template / Primer) l Trans. Start® Top. Taq DNA Polymerase Trans. Start® Tip Green q. PCR Super. Mix AQ 141 -02 5× 1 ml 230, 000 l Triple Blocking l Trans. Start® Top. Taq DNA Polymerase 부산, 경남지역 전문 대리점 바이올 (Bioall) Price Note 48039 부산광역시 해운대구 반여로 83 Tel 010 -8969 -3176 E-mail Fax bioall 16@naver. com 051 -980 -0680
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