XRay Facility Overview IMB XRay Facility IMB XRF
X-Ray Facility Overview IMB X-Ray Facility (IMB XRF) 410 -413 Kasha Laboratory www. sb. fsu. edu/~xray 850 -6448 Feb 18 & 19, 2009 © 2002 -09 Thayumanasamy Somasundaram 1
IMB X-Ray Facility (XRF) o o o IMB: Inter-disciplinary Institute Structural Biology & Computational Biophysics XRF: Core Facility [Est. 1993] Open and Free Access Safety & On-site Training Required Feb 18 & 19, 2009 © 2002 -09 Thayumanasamy Somasundaram 2
What XRF Can Do For You? Images courtesy of Michael Chapman Lab Crystal e- Density Feb 18 & 19, 2009 Diffraction Structure © 2002 -09 Thayumanasamy Somasundaram XRF can help you in Steps 3
X-Ray Generator & Mirror o o o Rigaku RU-H 2 R Cu Rotating Anode Brilliant Beam Osmic Confocal Mirror Wavelength Purity High Flux Feb 18 & 19, 2009 © 2002 -09 Thayumanasamy Somasundaram www. rigaku. com 4
X-Ray Detectors Imaging Plate & CCD o Rigaku R-Axis IIc o Two 19 cm 2 IPs o 8 minute Read-out o Mar. CCD 165 o Single CCD o 3 s Read-out Feb 18 & 19, 2009 © 2002 -09 Thayumanasamy Somasundaram 5
X-Ray (Cryo) Data Collection o Mar. CCD/Crystal. Clear o GUI Automated Analog» Digital o Oxford Cryo. System o o o Collect at 100°K Protects Crystals Sharper e. Density www. oxfordcryosystems. co. uk Feb 18 & 19, 2009 © 2002 -09 Thayumanasamy Somasundaram 6
Cryo. Crystallography o Flash Cool in Stream n n o For Immediate Collection Ease of Handling Flash Cool in Liquid n n For Storage For Shipping & Transport Flash Cool in Stream Flash Cool in Liquid Feb 18 & 19, 2009 © 2002 -09 Thayumanasamy Somasundaram 7
Amenities & Accessories o o o Crystal Room Cold Room Variable T Incubator Digital Imaging Synchrotron Time Feb 18 & 19, 2009 o o o Microscopes Cryo Tools Computer Licenses 3 D Graphics Sample Tools © 2002 -09 Thayumanasamy Somasundaram 8
Crystal & Data Services o o Screening & Crystallization Data Collection Strategy Crystal Storage & Transport Synchrotron Time & Usage Feb 18 & 19, 2009 o o o Data Processing & Visualization Data Interpretation Data Storage & Extraction © 2002 -09 Thayumanasamy Somasundaram 9
XRF-Contact Thayumanasamy “Soma”sundaram 414 Institute of Molecular Biophysics Florida State University Tallahassee, FL 32306 -4380 Phone: 850 -6448 | Fax: 850 -644 -7244 E-mail: tsomasundaram@fsu. edu Web: www. sb. fsu. edu/~xray Feb 18 & 19, 2009 © 2002 -09 Thayumanasamy Somasundaram 10
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