XRay Facility Overview BCH 4053 IMB XRay Facility

X-Ray Facility Overview [BCH 4053] IMB X-Ray Facility 410 -413 Kasha Laboratory www. sb. fsu. edu/~xray 850 -6448 March 26 & 27, 2007 © 2002 -2007 Thayumanasamy Somasundaram 1

IMB X-Ray Facility o o o Inter-disciplinary Institute Structural Biology Program Core Facility [Estd: 1993] No charge for use Open Access March 26 & 27, 2007 © 2002 -2007 Thayumanasamy Somasundaram 2

What XRF Can Do For You? Images courtesy of Michael Chapman Lab Crystal E Density March 26 & 27, 2007 Diffraction Structure © 2002 -2007 Thayumanasamy Somasundaram XRF can help you in Steps 3

X-Ray Generators o o Rigaku RU-H 2 R Cu Rotating Anode Single Wavelength Brilliant Beam www. rigaku. com March 26 & 27, 2007 © 2002 -2007 Thayumanasamy Somasundaram 4

X-Ray Detector 1 o o Imaging Plate (IP) Rigaku R-Axis IIc Two 19 cm 2 IPs 8 minute Read-out Variable Distance www. rigaku. com March 26 & 27, 2007 © 2002 -2007 Thayumanasamy Somasundaram 5

X-Ray Detector-2 Charge Coupled Device o o Mar. CCD 165 Single CCD 3 s Read-out Variable Distance www. mar-usa. com March 26 & 27, 2007 © 2002 -2007 Thayumanasamy Somasundaram 6

X-Ray Detector-3 Multiwire o o Xentronics X-1000 Gas-filled Accurate X-Ray Scattering www. bruker-axs. de March 26 & 27, 2007 © 2002 -2007 Thayumanasamy Somasundaram 7

X-Ray Monochromatization o o o Multi-layer Mirrors Osmic Confocal Wavelength Purity High Flux www. rigaku. com March 26 & 27, 2007 © 2002 -2007 Thayumanasamy Somasundaram 8

X-Ray Data Collection o o Cryo Coolers Oxford Cryo. System Now at 100°K Before at RT To Protect Crystals www. oxfordcryosystems. co. uk March 26 & 27, 2007 © 2002 -2007 Thayumanasamy Somasundaram 9

X-Ray Data Control Linux/Windows o o Mar. CCD/Crystal. Clear GUI Automated Analog» Digital March 26 & 27, 2007 © 2002 -2007 Thayumanasamy Somasundaram 10

Accessories Computers Cryo March 26 & 27, 2007 Software Hardware © 2002 -2007 Thayumanasamy Somasundaram 11

Amenities o o o Crystal Room Cold Room Variable T Incubator Digital Imaging Synchrotron Time Software Licenses Coldroom March 26 & 27, 2007 © 2002 -2007 Thayumanasamy Somasundaram 12

Crystal Services o o o o Crystal Screening Crystallization Data Collection Strategy Crystal Storage Crystal Transport Synchrotron Help March 26 & 27, 2007 © 2002 -2007 Thayumanasamy Somasundaram 13

Data Services o o o Data Data Processing Visualization Interpretation Archives Storage Extraction March 26 & 27, 2007 © 2002 -2007 Thayumanasamy Somasundaram 14

XRF-Contact Thayumanasamy “Soma”sundaram 414 Institute of Molecular Biophysics Florida State University Tallahassee, FL 32306 -4380 Phone: 850 -6448 | Fax: 850 -644 -7244 E-mail: soma@sb. fsu. edu Web: www. sb. fsu. edu/~soma March 26 & 27, 2007 © 2002 -2007 Thayumanasamy Somasundaram 15