WVU Flow Cytometry Single Cell Core Facility Presents

WVU Flow Cytometry & Single Cell Core Facility Presents: Laser Force Cytology for Rapid Viral Infectivity Measurements and Label-Free Single Cell Analysis Speaker: Dr. Colin Herbert Technical Operations at Luma. Cyte Precise and predictive analytics are fundamental to understanding biological processes, especially those as sophisticated and variable as viral vaccines and cell therapies. Current methods to measure viral infectivity are generally slow, labor intensive, subjective, and difficult to standardize, while autologous cell therapy represents a fundamental shift from “scaling-up” to larger manufacturing vessels as is done with traditional bioprocesses to “scaling-out”, the task of replicating a validated process thousands of times at the individual scale. Thus, there is a need for technologies that interrogate cells in a sensitive and precise manner to research, develop, and optimize complex biological systems and processes such as viral vaccines 1, cancer R&D 2, and cell therapy. Luma. Cyte’s Radiance. TM instrument fulfills this need through the use of Laser Force Cytology (LFC), a combination of advanced optics and microfluidics that analyzes single cells based upon their intrinsic biophysical and biochemical properties without the use of antibodies or fluorescent labels. LFC is sensitive to agents that perturb cellular structures or change biochemical composition. Functional viral infectivity measurements can be made in a fraction of the time as traditional assays or in near real-time throughout an ongoing infection. Phenotypic changes including cell differentiation, activation, apoptosis, and cytoskeletal stiffness can be measured, characterized, and used to identify cell populations. Additional applications of Luma. Cyte’s LFC platform include label-free biomarker development, gene therapy tool development (AAV and lentivirus production and transfection) and pre-clinical drug discovery. When: AUGUST 19, 2019 Time: 1: 00 PM Where: 2094 HSCN Contact: Kathy Brundage for details flowcore@hsc. wvu. edu