WP meeting Leafy Vegetables WP 3 Cadot Valrie
WP meeting Leafy Vegetables WP 3 Cadot Valérie GEVES : Variety and seed, study and control group Groupe d’Etude et de contrôle des Variétés Et des Semences V Cadot, : WP meeting Leafy Vegetables 27 th September 2007
A. Objectives u Evaluation for pests and diseases resistance u Evaluation for quality and abiotic characters u Inclusion in the databases to enable a more sustainable production methods and a better quality product V Cadot, : WP meeting Leafy Vegetables 27 th September 2007
Deliverables Deliverable date WP 3 for WP 3 Deliverable description Status Partner responsible On going P 0, P 1, P 2, P 3, P 4, P 5, P 7, P 8, P 9, P 10, P 12, P 13 Not yet due P 1, P 4, P 7, P 9, P 10, P 13 Year 1 D 16; month 12 Seeds of the selected material of the four crop groups sent to the partners involved Year 4 D 17; month 45 115 accessions of lettuce evaluated D 18; month 45 320 accessions of spinach evaluated Not yet due P 0, P 5 D 19; month 45 265 accessions of chicory evaluated Not yet due P 2, P 8, P 12 D 20; month 45 49 accessions of minor crops evaluated Not yet due P 3, P 5, P 9, P 12 Not yet due P 0, P 1, P 2, P 3, P 4, P 5, P 7, P 8, P 9, P 10, P 12, P 13 D 21; month 45 Evaluation data entered in a fixed format to be sent to the database managers V Cadot, : WP meeting Leafy Vegetables 27 th September 2007
Milestones for WP 3 Milestone date Milestone description Status Partner responsible Material selected for the evaluation activities of the four crop groups (see WP 1) Done (P 12 on going) Pall Not yet due P 0, P 1, P 2, P 4, P 5, P 7, P 8, P 9, P 10, P 12, P 13 Not yet due P 1, P 4, P 7, P 9, P 10, P 13 Year 1 month 12 Year 2 month 18 Evaluations started Year 3 month 36 70 accessions of lettuce evaluated month 36 210 accessions of spinach evaluated Not yet due P 0, P 5 month 36 170 accessions of chicory evaluated Not yet due P 2, P 8, P 12 month 36 30 accessions of minor crops evaluated Not yet due P 3, P 5, P 9, P 12 Year 4 See deliverables V Cadot, : WP meeting Leafy Vegetables 27 th September 2007 Not yet due
1. What is done by who? a) for pests and diseases resistance u Lettuce: 52 evaluations – Bremia lactucae P 4 (CZ), P 10 (GBR) – Root-knot nematodes (Meloidogyne sp. ) u Spinach: 250 evaluations – Downy mildew (Peronospora farinosa) u Chicory: P 13 (SLO) P 0 (NLD) 165 evaluations – Thielaviopsis basicola P 2 (FRA) – Alternaria cichorii P 2 (FRA) – Sclerotinia sclerotiorum P 2 (FRA) V Cadot, : WP meeting Leafy Vegetables 27 th September 2007
1. What is done by who? b) for quality and abiotic characters u u u Lettuce: 55 evaluations – Post harvest discoloration – Winter hardiness – Quality, taste (organoleptic) Spinach: 70 evaluations – Nitrate, oxalic acid, dry matter Chicory: 225 evaluations – Shape, color, taste, history, regionalism – Sesquiterpenes – Internal sensory characteristics Valerianella: 9 evaluations – Winter hardiness, taste Rucola: 40 evaluations – Nitrate, dry matter – Glucosinolate content V Cadot, : WP meeting Leafy Vegetables 27 th September 2007 P 1(GBR) P 7 (AUT) P 7(AUT) ? , P 9 (SLO) P 5 (DAN, SW) P 8 (CHE) P 12 (ITA) P 9 (SLO) P 5 (DAN, SW) P 12 (ITA)
Evaluation for P 0 (CGN) u u What has been done: Start made of discussing the evaluation of spinach accessions with the breeding companies involved. What will be done: Finalize the plans for the evaluation of spinach accessions. V Cadot, : WP meeting Leafy Vegetables 27 th September 2007
Evaluation for P 1 : started (WHRI) What has been done: u The selected gene bank accessions plus controls were assessed for post harvest discolouration following established experimental protocols. u Significant differences were found between the accessions for mean discolouration. The data is still being analysed as subsets for each day post harvest but so far significant differences have been found between accessions for each day analysed. V Cadot, : WP meeting Leafy Vegetables 27 th September 2007
Evaluation for P 2 (GEVES) u u What has been done: it was considered as done for P 2 and the material was sent to P 8. P 12 will ask fresh material from P 8 and if necessary, P 12 could ask seeds to P 2 and P 3 when he starts evaluation. What will be done: Finalize and harmonize with partners involved the scale of notation for evaluation. Coordinate the finalizing of evaluation plans for all the crops with the other leaders of species (P 0, P 1, P 3). V Cadot, : WP meeting Leafy Vegetables 27 th September 2007
Evaluation for P 4 (Palacky) u u u What has been done: Selection of 30 lettuce accessions for characterization, In June: Control of viability and pathogenicity of races of Bremis lactucae, pre-selection of isolates to be used for evaluation in a year 2. What will be done: Evaluation starts in year 2. V Cadot, : WP meeting Leafy Vegetables 27 th September 2007
Evaluation for P 7 (Arche Noah) u u What has been done: First selection of accessions to be tested for winter hardiness. What will be done: Developing criteria for evaluate winter hardiness. V Cadot, : WP meeting Leafy Vegetables 27 th September 2007
Evaluation for P 9 (USOFA) u What has been done: Evaluation for important property and quality for us. V Cadot, : WP meeting Leafy Vegetables 27 th September 2007
Evaluation for P 10 (HDRA) u u What has been done: Material for evaluation (lettuce) already selected (all 17 Heritage Seed Library accessions). What will be done: Nothing planned until year 2 V Cadot, : WP meeting Leafy Vegetables 27 th September 2007
Evaluation for P 12 (University of Bologna) u u The aspect of sesquiterpenes is still open. In fact most of what was published in literature did not remain in practical. Some delay will occur in the starting of the real evaluation phase, but this will affect only the time development of the program and not the global results. Bottlenecks: restudy analytical aspect for sesquiterpenes. V Cadot, : WP meeting Leafy Vegetables 27 th September 2007
Inquiry about the scale of notation for WP 3 *Which scale of notation ? * Which data format will be sent? 1. A note with a level of expression associated. Ex : 1= susceptible ; 9 = resistant 2. A measured note. Ex : 50 cm 3. A file linked to the accession number but not advised to sort out 4. A text included in the database as “Remarks” 5. A combination of these 4 possibilities: Indicate which combination you wish. Ex : 5=1+2+4 V Cadot, : WP meeting Leafy Vegetables 27 th September 2007
Scale for pests and diseases : harmonization on going Species Partners Pests & Diseases Lettuce P 4 (Palacky Univ. ) Ales Bremia lactucae P 10 (HDRA) Margi Which scale of notation? Which data format will be sent * (1 to 5) For the assessment of sporulation intensity on lettuce cotyledons, a scale 0 -3 will be used: 0 = no visible conidiophores on leaves; 1 = limited sporulation, sporadic conidiophores present; 2 = <50% of cotyledon area covered with conidiophores; 3 = >50% of cotyledon area covered with conidiophores. 5 = 1+2+3+4 Bremia lactucae 1=100% infection 9= 0% infection 5=1+4 P 13 (KIS) Vladimir Nematodes R = resistant (GI < 2, Pf < 1) HR = Hypersensitive reaction (GI > 2, Pf < 1) S = Susceptible (GI > 2, Pf > 1) 1 Spinac h P 0 (CGN) Liesbeth Downy mildew, 3 -5 races Chicory P 2 - SNES Valérie Thielaviopsis basicola R = resistant ; S = Susceptible According statistics: Necrotic Root Index: 0= no symptoms to 4=100% necrotics 5=1+4 P 2 - GEVES Valérie Alternaria cichorii R = resistant ; S = Susceptible According statistics: 1=0% infection to 9= 100% infection 5=1+4 P 2 -FNPE Valérie Sclerotinia sclerotiorum R = resistant ; S = Susceptible According statistics: classes of necrosis: 0= no symptoms to 5= Chicon wilt V Cadot, : WP meeting Leafy Vegetables 27 th September 2007 The data will be presented in: 1) Binary form (+=susceptible, -=resistant); 2) Quantitatively (the final value of sporulation intensity (disease intensity) will be expressed as the percentage of the maximum score); 3) Table will be prepared with these data, i. e. 30 accessions screened by 20 different isolates). 5=1+4
Scale for quality and abiotic characters : a few answers 1/2 Species Partners Evaluation Lettuce P 1 (HRI) Post harvest discoloration Dave Which scale of notation? Which data format Winter hardiness 1) 1=no winterhardiness ; 9=complete winter hard 2) % for how much plants survived 3) cm for head measure 4) text for remarks Same scale of notation than P 9 Franziska Taste organoleptic P 7 (Arche Noah) Quality Same scale of notation than P 9 1 Type of lettuce (1 = butterhead, 2 = crisphead-batavia, 3 = crisphead-iceberg, 4 = cutting (leaf), 5 = cos (Roman)) Earliness - time of harvest maturity, evaluation 1 to 9 (3 = early, 5 = medium, 7 = late) Beginning of bolting, evaluation 1 to 9 (3 = late, 5 = medium, 7 = early) Plant height in cm (average of 10 plants) Plant width in cm (average of 10 plants) Plant weight in g (average of 10 plants) Plant colour (1 = yellow, 2 = yellow green, 3 = green, 4 = red) Colour intensity, evaluation 1 to 9 (3 = light, 5 = medium, 7 = dark) Anthocyanin coloration (1[XXX 1] = present, 2 = absent) Head density, evaluation 1 to 9 (3 = dense, 5 = medium, 7 = loose) Leaf blistering, evaluation 1 to 9 (1 = absent or very weak, 5 = medium, 9 = very strong) Marketable part weight in g (average of 10 plants) Marketable yield (t/ha) (average of 3 repetitions of cca 20 plants) Total yield (t/ha) (average of 3 repetitions of cca 20 plants) 1 P 7 (Arche Noah) Franziska P 7 (Arche Noah) 1+2+4 Franziska P 9 (USOFA) and P 13 (KIS) Taste organoleptic Mihaela and Vladimir P 9 (USOFA) and P 13 (KIS) Quality Mihaela and Vladimir V Cadot, : WP meeting Leafy Vegetables 27 th September 2007
Scale for quality and abiotic characters : a few answers 2/2 Species Partners Evaluation Spinach P 5 (NGB) Katarina Nitrate P 5 (NGB) Katarina Oxalic acid P 5 (NGB) Katarina Dry matter P 8 (Pro Specie Rara) Bela Shape, color, taste, history, regionalism 3? P 12 (UNIBO) Filippo Sesquiterpene lactones external 2 P 12 (UNIBO) Filippo Internal sensory characteristics 1 P 9 (USOFA) Mihaela Winter hardiness P 9 (USOFA) Mihaela Taste (organoleptic) P 5 (NGB) Katarina Nitrate P 5 (NGB) Katarina Dry matter P 12 (UNIBO) Filippo Glucosinolate content Chicory Valerianella Rucola V Cadot, : WP meeting Leafy Vegetables 27 th September 2007 Which scale of notation? Which data format? 1
What about dissemination of results? V Cadot, : WP meeting Leafy Vegetables 27 th September 2007
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