Winning tools Winning Processes Our toolbox Hydrolase Kinetic

Winning tools – Winning Processes Our toolbox Hydrolase – Kinetic resolution of Alcohols, Amine, amides and esters

Our Toolkit - Lipase Screening Reaction check by TLC prior to HPLC Substrate Screening Ø 40 enzymes tested Ø Very selective - Enantiomeric Ratio (E) E>20 suitable for Large scale manufacture (Green)

Winning tools – Winning Processes Our toolbox Hydrolase – Kinetic resolution of alcohols, amines, amides and esters Over 40 -best-in-class enzymes (lipase, protease, hydrolase) all in one screening kit. Screening kits are free of charge (courier charges apply) Collaborations with world leading providers giving optimised cost structure for you:

Winning tools – Winning Processes Our toolbox Ready November Alcohol Dehydrogenase – Reduction of prochiral ketone to chiral alcohol Cofactor is expensive – recycle is required

Winning tools – Winning Processes Our toolbox Ready November Alcohol Dehydrogenase – Reduction of prochiral ketone to chiral alcohol Waste Ketone Eg acetone NAD(P)H Cheap donor alcohol eg Isopropanol In situ recycle of cofactor usingle enzyme NAD(P)+

Winning tools – Winning Processes Our toolbox Ready November Alcohol Dehydrogenase – Reduction of prochiral ketone to chiral alcohol Waste product Gluconate Ethanol CO 2 NAD(P)H Enzyme 2 Many options eg Glucose Pyruvate formate In situ recycle of cofactor using dual enzymes NAD(P)+

Winning tools – Winning Processes Our toolbox Ready December • Nitrilase – Selective hydrolysis of nitriles to acids Racemic Nitrile Chiral Acid • Panel of R and S-selective Nitrilases available • In house enzymes and through collaboration

Winning tools – Winning Processes Our toolbox Ready December Nitrile Hydratase – Selective hydrolysis of nitriles to amides Racemic Nitrile Chiral Amide Panel of R and S-selective Nitrile Hydratases available In house enzymes and through collaboration