Where do photons go When they come out

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Where do photons go When they come out of the beamline and hit my

Where do photons go When they come out of the beamline and hit my crystal

Elastic scattering

Elastic scattering

Elastic scattering

Elastic scattering

Inelastic scattering

Inelastic scattering

Photonuclear absorption

Photonuclear absorption

Photonuclear absorption

Photonuclear absorption

Photoelectric absorption

Photoelectric absorption

Photoelectric absorption e- +

Photoelectric absorption e- +

Fluorescence +

Fluorescence +

Fluorescence e- +

Fluorescence e- +

Photoelectric absorption

Photoelectric absorption

Photoelectric absorption e- +

Photoelectric absorption e- +

Secondary ionization e-

Secondary ionization e-

Secondary ionization e- + e-

Secondary ionization e- + e-

Secondary ionization

Secondary ionization

Secondary ionization

Secondary ionization

Secondary ionization + e-

Secondary ionization + e-

Secondary ionization e- e- + e- e-

Secondary ionization e- e- + e- e-

Secondary ionization e- e- + + + e- + + ee-

Secondary ionization e- e- + + + e- + + ee-

Secondary ionization e- e- + + + e- + + ee-

Secondary ionization e- e- + + + e- + + ee-

Secondary ionization e- e- e- + + + e- + + ee-

Secondary ionization e- e- e- + + + e- + + ee-

Where do photons go? Protein 1 A x-rays Transmitted (98%) beamstop

Where do photons go? Protein 1 A x-rays Transmitted (98%) beamstop

Where do photons go? Protein 1 A x-rays elastic scattering (6%) Transmitted (98%) beamstop

Where do photons go? Protein 1 A x-rays elastic scattering (6%) Transmitted (98%) beamstop

Where do photons go? Protein 1 A x-rays elastic scattering (6%) Transmitted (98%) beamstop

Where do photons go? Protein 1 A x-rays elastic scattering (6%) Transmitted (98%) beamstop inelastic scattering (7%)

Where do photons go? Protein 1 A x-rays elastic scattering (6%) Transmitted (98%) beamstop

Where do photons go? Protein 1 A x-rays elastic scattering (6%) Transmitted (98%) beamstop inelastic scattering (7%) Re-emitted (99%) Absorbed (~0%)

Where do photons go? Protein 1 A x-rays elastic scattering (6%) Transmitted (98%) beamstop

Where do photons go? Protein 1 A x-rays elastic scattering (6%) Transmitted (98%) beamstop inelastic scattering (7%) Re-emitted (99%) Absorbed (~0%) Photoelectric (87%)

Where do photons go? Protein 1 A x-rays elastic scattering (6%) Transmitted (98%) beamstop

Where do photons go? Protein 1 A x-rays elastic scattering (6%) Transmitted (98%) beamstop inelastic scattering (7%) Re-emitted (99%) Absorbed (~0%) Photoelectric (87%) Re-emitted (~0%) Absorbed (99%)

Where do photons go? Protein 1 A x-rays elastic scattering (6%) Transmitted (98%) beamstop

Where do photons go? Protein 1 A x-rays elastic scattering (6%) Transmitted (98%) beamstop inelastic scattering (7%) Re-emitted (99%) Absorbed (~0%) Photoelectric (87%) Re-emitted (~0%) useful/absorbed energy: 7. 3% Absorbed (99%)

Where do photons go? Seleno. Protein 1 A x-rays elastic scattering (6%) Transmitted (97%)

Where do photons go? Seleno. Protein 1 A x-rays elastic scattering (6%) Transmitted (97%) beamstop inelastic scattering (5%) Re-emitted (99%) Absorbed (~0%) Photoelectric (88%) Re-emitted (~0%) useful/absorbed energy: 7. 6% Absorbed (99%)

Where do photons go? Seleno. Protein 0. 92 A x-rays just above the edge

Where do photons go? Seleno. Protein 0. 92 A x-rays just above the edge elastic scattering (2%) Transmitted (91%) beamstop inelastic scattering (2%) Re-emitted (99%) Absorbed (~0%) Photoelectric (96%) Re-emitted (57%) useful/absorbed energy: 5. 1% Absorbed (44%)

Where do photons go? Seleno. Protein 0. 97 A x-rays far above the edge

Where do photons go? Seleno. Protein 0. 97 A x-rays far above the edge elastic scattering (2%) Transmitted (90%) beamstop inelastic scattering (2%) Re-emitted (99%) Absorbed (~0%) Photoelectric (96%) Re-emitted (60%) useful/absorbed energy: 5. 1% Absorbed (40%)

Background scattering 14 16 The form-factor of the cryostream 12 4 6 8 10

Background scattering 14 16 The form-factor of the cryostream 12 4 6 8 10 theoretical 2 0 Electron equivalents measured 20 10 5 4 3 2. 5 2 1. 8 1. 6 Resolution (Ǻ) 1. 4 1. 2 1

Background scattering Photons/s/pixel Se edge with detector at 100 mm 7. 5 3. 8

Background scattering Photons/s/pixel Se edge with detector at 100 mm 7. 5 3. 8 2. 5 1. 9 Resolution (Ǻ) 1. 5 1. 2 1. 1