Where do photons go When they come out
- Slides: 35
Where do photons go When they come out of the beamline and hit my crystal
Elastic scattering
Elastic scattering
Inelastic scattering
Photonuclear absorption
Photonuclear absorption
Photoelectric absorption
Photoelectric absorption e- +
Fluorescence +
Fluorescence e- +
Photoelectric absorption
Photoelectric absorption e- +
Secondary ionization e-
Secondary ionization e- + e-
Secondary ionization
Secondary ionization
Secondary ionization + e-
Secondary ionization e- e- + e- e-
Secondary ionization e- e- + + + e- + + ee-
Secondary ionization e- e- + + + e- + + ee-
Secondary ionization e- e- e- + + + e- + + ee-
Where do photons go? Protein 1 A x-rays Transmitted (98%) beamstop
Where do photons go? Protein 1 A x-rays elastic scattering (6%) Transmitted (98%) beamstop
Where do photons go? Protein 1 A x-rays elastic scattering (6%) Transmitted (98%) beamstop inelastic scattering (7%)
Where do photons go? Protein 1 A x-rays elastic scattering (6%) Transmitted (98%) beamstop inelastic scattering (7%) Re-emitted (99%) Absorbed (~0%)
Where do photons go? Protein 1 A x-rays elastic scattering (6%) Transmitted (98%) beamstop inelastic scattering (7%) Re-emitted (99%) Absorbed (~0%) Photoelectric (87%)
Where do photons go? Protein 1 A x-rays elastic scattering (6%) Transmitted (98%) beamstop inelastic scattering (7%) Re-emitted (99%) Absorbed (~0%) Photoelectric (87%) Re-emitted (~0%) Absorbed (99%)
Where do photons go? Protein 1 A x-rays elastic scattering (6%) Transmitted (98%) beamstop inelastic scattering (7%) Re-emitted (99%) Absorbed (~0%) Photoelectric (87%) Re-emitted (~0%) useful/absorbed energy: 7. 3% Absorbed (99%)
Where do photons go? Seleno. Protein 1 A x-rays elastic scattering (6%) Transmitted (97%) beamstop inelastic scattering (5%) Re-emitted (99%) Absorbed (~0%) Photoelectric (88%) Re-emitted (~0%) useful/absorbed energy: 7. 6% Absorbed (99%)
Where do photons go? Seleno. Protein 0. 92 A x-rays just above the edge elastic scattering (2%) Transmitted (91%) beamstop inelastic scattering (2%) Re-emitted (99%) Absorbed (~0%) Photoelectric (96%) Re-emitted (57%) useful/absorbed energy: 5. 1% Absorbed (44%)
Where do photons go? Seleno. Protein 0. 97 A x-rays far above the edge elastic scattering (2%) Transmitted (90%) beamstop inelastic scattering (2%) Re-emitted (99%) Absorbed (~0%) Photoelectric (96%) Re-emitted (60%) useful/absorbed energy: 5. 1% Absorbed (40%)
Background scattering 14 16 The form-factor of the cryostream 12 4 6 8 10 theoretical 2 0 Electron equivalents measured 20 10 5 4 3 2. 5 2 1. 8 1. 6 Resolution (Ǻ) 1. 4 1. 2 1
Background scattering Photons/s/pixel Se edge with detector at 100 mm 7. 5 3. 8 2. 5 1. 9 Resolution (Ǻ) 1. 5 1. 2 1. 1
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