Whats magnified Whats magnified Whats magnified Microscopy Microscopes

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What’s magnified?

What’s magnified?

What’s magnified?

What’s magnified?

What’s magnified?

What’s magnified?

Microscopy

Microscopy

Microscopes • “micro” means small! • “scope” means watch/look! Microscopes were first developed as

Microscopes • “micro” means small! • “scope” means watch/look! Microscopes were first developed as entertainment for the rich.

Light Microscopes • Focuses visible light rays • Can be used on live or

Light Microscopes • Focuses visible light rays • Can be used on live or dead specimens • Dyes & fluorescent markers help for viewing • Magnify up to 1000 X on the ones we have! • Compound Light Microscopes

Light Micrographs Plant Human Chromosomes

Light Micrographs Plant Human Chromosomes

The Compound Light Microscope The word compound means to increase! A compound microscope has

The Compound Light Microscope The word compound means to increase! A compound microscope has 2 lenses to increase magnification!

Parts of the Microscope 1. Ocular Lens: eyepiece (10 x magnification), first lens 2.

Parts of the Microscope 1. Ocular Lens: eyepiece (10 x magnification), first lens 2. Arm: connects base & body tube – Must hold arm while carrying microscope! 3. Course Adjustment Knob: roughly focuses object – Used to find specimen

Parts of the Microscope 4. Fine Adjustment Knob: small knob, finely focuses specimen –

Parts of the Microscope 4. Fine Adjustment Knob: small knob, finely focuses specimen – Use after object has been found! 5. Power Cord: must be wrapped around arm after use 6. Nosepiece: holds 3 objective lenses

Parts of the Microscope 7. Objective Lens: second lens – – Scanning Lens (4

Parts of the Microscope 7. Objective Lens: second lens – – Scanning Lens (4 x) Low Power (10 x) High Power (40 x) Oil Immersion (100 x)

Parts of the Microscope 8. Stage Clips: holds slide in place 9. Stage: flat

Parts of the Microscope 8. Stage Clips: holds slide in place 9. Stage: flat plate where slides are held 10. Diaphragm: regulates the amount of light exposed to the specimen

Parts of the Microscope 11. Condenser Lens: focuses light on specimen 12. Power Switch:

Parts of the Microscope 11. Condenser Lens: focuses light on specimen 12. Power Switch: turns light on & off 13. Base: support of the microscope

Field of View • Total area visible through the ocular lens The higher the

Field of View • Total area visible through the ocular lens The higher the magnification, the smaller the field of view.

Total Magnification = ocular lens x objective lens If the 40 x objective is

Total Magnification = ocular lens x objective lens If the 40 x objective is in place… TM = 400 x, because (10 x 40 = 400)

Focusing the Microscope • Locate the slide specimen at scanning power. • Then move

Focusing the Microscope • Locate the slide specimen at scanning power. • Then move the area to be viewed to the center of the field of view. • Make sure the slide is in perfect focus at scanning power before moving up to low power. • Turn your objective without moving the slide or stage. • You will slightly adjust the image at the next objective. • At high power use only the fine focus knob and do not turn it more than 1/2 turn.

Important! Microscope Rules • Always carry with 1 hand on arm & 1 hand

Important! Microscope Rules • Always carry with 1 hand on arm & 1 hand on base! • Use a slide & coverslip. • Clean Up: – Always return to scanning (lowest) power! – Always lower stage all the way! – Unplug, wrap cord, & cover

Intro. Microscope Lab NO HORSEPLAY!! • Follow the directions carefully… don’t skip over things

Intro. Microscope Lab NO HORSEPLAY!! • Follow the directions carefully… don’t skip over things just because you don’t feel like reading!