Welcome to the Media Cybernetics 2003 Dealer Conference
Welcome to the Media Cybernetics 2003 Dealer Conference Silver Spring, San Diego, Munich and Singapore August/September 2003
Introducing Image-Pro Plus 5. 0! Agenda for 2 -Day Program • Day One – 9: 00 AM Welcome & Opening Remarks – 10: 00 AM-Noon Image Pro Plus Five-0 – Noon-1: 00 PM Lunch – 1: 00 -2: 00 PM Image Pro Plus Five-0 (Cont. ) – 2: 00 -3: 00 PM Sharp. Stack Five-0 – 3: 00 -4: 00 PM 3 D Constructor Five-0 – 4: 00 -5: 00 PM Scope-Pro Five-0 • Day Two – 9: 00 -11: 00 AM What is IQbase? – 11: 00 -Noon How Can WE Demonstrate & Sell IQbase? – Noon-1: 00 PM Lunch – 1: 00 -2: 00 PM Business Meeting – 2: 00 PM Adjourn Confidential - Do not copy or distribute
Image-Pro Plus 5. 0 More Answers on your Images! Image-Pro Plus Five-O
Image-Pro Plus 5. 0 Some Highlights
Introducing Image-Pro Plus 5. 0! Exciting New Features • Alignment Correction • Automatic Tiling and Stitching • Tracking – Automatic and manual – Measurement Display Options Confidential - Do not copy or distribute
Introducing Image-Pro Plus 5. 0! Exciting New Features • Improved Stack Format Support – Handle stacks better and automatically assign colors where applicable. • Color Composite Improvements – Customize Dyes and Colors – Image Set and Existing Image Knowledge • New Basic Capture Dialog Confidential - Do not copy or distribute
Introducing Image-Pro Plus 5. 0! Exciting New Features • New Processing and Display Tools – Despeckle Filter – L*A*B Color – Scale Bar – EDF Test Strips – – Local Zoom Synchronized Playback of Sequences Synchronized Pan and Zoom Surface Plot Overlay from EDF Confidential - Do not copy or distribute
Introducing Image-Pro Plus 5. 0! Exciting New Features • Snap to New Image – Allows users to automatically burn measurement overlays onto an image • Set Manager – Multidimensional viewer - includes aspects of the improved sequence file support • Spatial Calibration Improvements – List Management – System Calibration – Wizard Confidential - Do not copy or distribute
Introducing Image-Pro Plus 5. 0! Exciting New Features • Workspace Context Menu Additions – Local Zoom, dye tints, set capture AOI appear in context menu • Version Information – Helps tech support know which versions of Image. Pro Plus and any plug-ins are running • Default Settings Folder – Location where all settings and environment information is stored for the product. Confidential - Do not copy or distribute
Introducing Image-Pro Plus 5. 0! Exciting New Features • Objective Information File – Important for those that work with microscopes; links to Scope-Pro and AFA • Display Range/Contrast Enhancement Interaction – User interface enhancement to make these tools less independent • Dye Management – Allows for custom dyes and for user to define color by emission wavelength – Pre-Defined Dyes – Add New Dyes – Tint Commands Confidential - Do not copy or distribute
Introducing Image-Pro Plus 5. 0! Exciting New Features • New Graphical User Interface • Toolbar Changes – Toolbar has added functionality • New Menu Structure – Menu is more logical for new users. Confidential - Do not copy or distribute
Introducing Image-Pro Plus 5. 0! Exciting New Features • Very New! Work. Flow Manager – User defined function – Toolbar oriented • Very New! User Identities – Login – Basic, Full, Biological, Industrial defaults Confidential - Do not copy or distribute
Image-Pro Plus 5. 0 Key Selling points Hands On
IPP 5. 0 Alignment Correction • Align images or a sequence in translation, rotation, and/or scaling. • Potential applications would be alignment of: – images taken over time with movement – channels of multiple images from a beam splitter – Serial sections – correcting for offset angle of a Greenough (offaxis) stereo microscope Confidential - Do not copy or distribute
IPP 5. 0 Alignment Correction Confidential - Do not copy or distribute
IPP 5. 0 Alignment Correction Confidential - Do not copy or distribute
IPP 5. 0 Alignment Correction Confidential - Do not copy or distribute
New Tools from the Solutions Zone Other Tools • Synchronized Playback of Sequences • Synchronized Pan and Zoom Confidential - Do not copy or distribute
New Tools from the Solutions Zone EDF Tools • EDF Test Strips and EDF Surface Plot Confidential - Do not copy or distribute
New Tools from the Solutions Zone EDF Tools Confidential - Do not copy or distribute
Automatic Tiling and Stitching • Align and stitch images together using a Fourier transform edge detection algorithm based upon both pattern and contrast matching • Options provide for the insertion of blank images, image order modification, and manual adjustment of pane location • This will be useful for constructing panoramic views or for linking together multiple images taken with a motorized or non-motorized stage. Confidential - Do not copy or distribute
Automatic Tiling and Stitching Confidential - Do not copy or distribute
Automatic Tiling and Stitching Confidential - Do not copy or distribute
Automatic Tiling and Stitching Confidential - Do not copy or distribute
Tracking • Manual Tracking – Click on objects frame by frame • Automatic Tracking – Highlight objects of interest and let Image-Pro do the heavy lifting • Graphs generated automatically – Cumulative and point to point options • Many Measurement Options – Distance, velocity, etc. Confidential - Do not copy or distribute
Tracking Confidential - Do not copy or distribute
Tracking Confidential - Do not copy or distribute
NEW Acquire Dialog! • New Basic Capture Dialog – Improved Nomenclature – Vertically aligned to conserve real estate – Most-used controls in one basic dialog *AND* – Auto. Set button – a one-button solution which: • • Confidential - Do not copy or distribute Resets camera Performs Auto-White Balance (color only) Calculates Auto-Exposure Opens preview pane.
NEW Acquire Dialog! Quickly assign and change settings Lock Preview and Acquire Settings Confidential - Do not copy or distribute Auto. Set!
NEW Acquire Dialog! Confidential - Do not copy or distribute
Spatial Calibration Improvements • System Calibration applied when a new image is captured in Image-Pro, Scope-Pro, or AFA • New Calibration Wizard • Automatic correction for binning in Scope-Pro Confidential - Do not copy or distribute
Spatial Calibration Improvements Confidential - Do not copy or distribute
Spatial Calibration Improvements Confidential - Do not copy or distribute
New Tools from the Solutions Zone Scale Bar • Color and orientation are easily chosen Confidential - Do not copy or distribute
New Tools from the Solutions Zone Local Zoom Confidential - Do not copy or distribute
Measurement Overlay • Allows users to save measurement overlays burned onto an image Press to snap measurements to image Confidential - Do not copy or distribute
Workspace Context Menu Additions - Right-click features • Local Zoom • Dye Tints • Dye Management – Allows for custom dyes and for user to define color by emission wavelength – Pre-Defined Dyes – Add New Dyes – Tint Commands Confidential - Do not copy or distribute
Color Composite Improvements – Customize Dyes and Colors – Preset Dye List – Links to Set and Image Information Confidential - Do not copy or distribute
Color Composite Improvements Press to create new dye Confidential - Do not copy or distribute
Color Composite Improvements Select color by visual inspection or emission wavelength! Confidential - Do not copy or distribute
Settings Improvements • Default Settings Folder – All settings and environment information is stored in this location • Lens Information File – Linked to acquisition in Scope-Pro and AFA Confidential - Do not copy or distribute
New Workflow Manager User-Defined Workflow Toolbar – Use Existing Menu Icons – Create New Icons – Include Self-Contained Macros without External Parameters Confidential - Do not copy or distribute
New Workflow Manager Confidential - Do not copy or distribute
Sharp Stack 5. 0
Sharp Stack 5. 0 • Improved Speed of calculations • Spherical Aberration correction • Included Algorithms – No Neighbor – Nearest Neighbor – Inverse Filtering Confidential - Do not copy or distribute
Sharp Stack 5. 0 Confidential - Do not copy or distribute
Sharp Stack 5. 0 Confidential - Do not copy or distribute
Supplementary Auto. Quant Modules • Sharp. Stack meets the majority market needs • Auto. Deblur Gold WF (widefield fluorescence) – Contains blind (2 D & 3 D) deconvolution methods • Auto. Deblur Gold CF (confocal fluorescence) – Contains confocal deconvolution methods • Auto. Deblur Gold WCF (all of the above) • Auto. Deblur 2 D (only single plane blind deconvolution) Confidential - Do not copy or distribute
3 D-Constructor 5. 0
3 D-Constructor 5. 0 • More Visualization effects • Manual Measurements • Automatic Measurements – 3 D Count/Size – Histogram object selection • 4 D Measurements • More Macros Confidential - Do not copy or distribute
3 D-Pro Series Competition • Very high prices • Small companies with mixed support capability • Mixed distribution strategy • Poor dealer relationships • Poor integration into mainstream 2 D image analysis packages Confidential - Do not copy or distribute
3 D-Pro Series 3 D Constructor: Volume Rendering Confidential - Do not copy or distribute
3 D-Constructor Manual Measurements Confidential - Do not copy or distribute
3 D-Constructor Automatic Volumetric Measurements Confidential - Do not copy or distribute
3 D-Constructor Automatic Volumetric Measurements Over Time 4 D Tracking of objects!! Confidential - Do not copy or distribute
Scope. Pro 5. 0
Scope-Pro 5. 0 Improvements • Improved minimal dialogs to save real estate • Acquire up to 999 Z-stack images • X-Y stage initialization retained between sessions • Calibration Improvements – Light path and magnification changers modify calibrations • Site map for user defined capture regions Confidential - Do not copy or distribute
Scope-Pro 5. 0 Confidential - Do not copy or distribute
Scope-Pro 5. 0 Confidential - Do not copy or distribute
AFA 4. 5 Advanced Fluorescence Acquisition
Advanced Fluorescence Acquisition (AFA) For customers who acquire complex image sets • Multiple color channels (usually fluorescence) or microscope modes • Time-lapse, motion studies • Stage position – Multiwell plates – Area scans – User defined locations • Multiple focal planes (Z-stack) Confidential - Do not copy or distribute
Advanced Fluorescence Acquisition (AFA) Enhanced Capabilities Time-lapse Multi-channel Multiwell plates Z-stack Scan area User-defined Stage Positions Confidential - Do not copy or distribute
Advanced Fluorescence Acquisition (AFA) Interface: First Use Confidential - Do not copy or distribute
Advanced Fluorescence Acquisition (AFA) Interface: Preview Tab Confidential - Do not copy or distribute
Scope-Pro Device Updates • Check our website for device updates Confidential - Do not copy or distribute
Camera Kit Update
Camera Kit Products • • • Cool. SNAPcf Evolution LC Evolution VF (NEW!) Evolution MP Evolution QEi Confidential - Do not copy or distribute
Cool. SNAP-Pro cf Kits • • 1392 X 1040 pixels 12 -bit chip, Cooled 5 C below ambient Available in Bayer color or monochrome or with CRI Micro*Color Faster readout speed (20 Mhz. ) Preview at 10 -12 frames/sec. full resolution mono Significantly Improved Sensitivity! Reduced dark current noise down to <2 electrons! Supports longer integration for fluorescence applications Confidential - Do not copy or distribute Camera List - $4, 999 Dealer Discounts up to 36%!
Evolution LC™ Integrated Camera Kit • Digital alternative to video microscopy • Available in monochrome and color • Features – Fully integrated with your choice of Image-Pro software – Fire. Wire (IEEE 1394) C-mount camera – 1. 3 megapixel array – Up to 14 fps at 1280 x 1024 res. , 30 fps at 640 x 480 – Laptop Kit Confidential - Do not copy or distribute
Evolution VF - cooled & uncooled version - mono & color • Features – Designed for high resolution brightfield scientific and industrial applications. – A progressive scan interline CCD sensor gives a resolution of 1. 4 million pixels in a 12 -bit digital output. – High-speed low noise electronics provide linear digital data – Frame rates of up to 110 fps in region of interest and binning. – The IEEE 1394 Fire. Wire™ digital interface allows ease of use Confidential - Do not copy or distribute
Evolution VF - Specifications CCD Sensor Light Sensitive Pixels 1. 4 million, 1392 x 1040 Binning Modes 2 x 2, 4 x 4, 8 x 8 ROI (Region Of Interest) From 1 x 1 pixels up to full resolution, continuously variable Exposure/Integration Control 12µs to 17. 9 min. in 1µs increments Sensor Type Sony ICX 205 Progressive Scan Interline CCD, Monochrome, or Color Pixel Size 4. 65µm x 4. 65µm Linear Full Well 10, 000 e- Read Noise 12 e- Cooling Available Yes Cooling Type Peltier Thermoel. cooling 25 degrees Celsius below ambient Digital Output 12 -bit Readout Frequency 20, 10, 5, 2. 5 MHz Frame Rate 10 fps full resolution @ 12 -bits, 165 fps maximum with binning and ROI Confidential - Do not copy or distribute
Evolution MP™ Integrated Color Camera Kit • High resolution 5. 5 megapixel color camera • Features – Fully integrated with your choice of Image-Pro software – Fire. Wire (IEEE 1394) C-mount camera – 3 -5 fps at 2560 x 1920(30 -bit); 25 fps at 640 x 480 – Option for cooling – No interpolation or Pixel shifting Confidential - Do not copy or distribute
Evolution MP™ Integrated Color Camera Kit Imaging Chip Type 2/3” Sony ICX-282 2 to 1 interlaced CCD color Resolution 2588 x 1960 Pixel Size 3. 4µm x 3. 4µm Readout Noise 9. 8 electrons Maximum Frame Rate 3 -5 fps at 2560 x 1920 (36 -bit) Shutter Electronic shutter, no moving parts Cooling Type Optional Peltier thermoelectric cooling to 10°C below ambient Physical Weight 710 g Lens mount C-mount, 2/3” optical format for microscope or camera lens, plus a 1/4”-20 tripod mount Interface Operating System Windows® 98 SE, ME, 2000, XP Data Format 10 -bit Interface Type IEEE 1394 Fire. Wire Maximum Data Rate 20 MHz at 8 -bit and 10 MHz at 10 bit Confidential - Do not copy or distribute
Evolution QEi™ Integrated Camera Kit • High Quantum Efficiency camera • Features – Fully integrated with your choice of Image-Pro software – Fire. Wire (IEEE 1394) C-mount camera – 5 fps at 1360 x 1036(12 -bit)10 fps at 1360 x 1036(8 -bit) Confidential - Do not copy or distribute
Evolution QEi™ Integrated Camera Kit Imaging Chip Type 2/3” Sony ICX-285 progressive scan interline CCD monochrome Resolution 1360 x 1036 Light Sensitive Pixels Pixel Size 6. 45µm x 6. 45µm Readout Noise 8 electrons Dark Current 0. 15 electrons/pixel/sec cooled Maximum Frame Rate 10 fps full resolution in 8 -bit, 5 fps in 12 -bit. Higher speed with binning and ROI functions (up to 4 x 4 binning) Shutter Electronic shutter, no moving parts Cooling Type Peltier thermoelectric cooling to 25°C below ambient Physical Lens mount C-mount, 2/3” optical format Interface Operating System Windows® 98 SE, ME, 2000, XP Data Format 12 -bit Interface Type IEEE 1394 Fire. Wire Maximum Data Rate 10 MHz and 20 MHz selectable at 12 -bit Confidential - Do not copy or distribute
IQbase Extracting Information From Images
Image-Pro IQbase • Information and data management software for individuals and companies that need a flexible and powerful database solution Confidential - Do not copy or distribute
Image-Pro IQbase • Object-Oriented Database – More powerful and faster searches than relational databases found in most competitive products – Compatible with SQL, Oracle, and other databases – Can be fully integrated with the world’s leading scientific image analysis software, Image-Pro Plus, which contains numerous input options, measurement and analysis tools, and world-class support Confidential - Do not copy or distribute
Image-Pro IQbase - Versatile • Data can be stored in a variety of locations • Data is organized according to the users needs and desires • Saves Time Confidential - Do not copy or distribute
Image-Pro IQbase - Versatile • Can work with MS-SQL, Oracle and other database types • Data can be made available to web servers for customers/collaborators to view over the world wide web Confidential - Do not copy or distribute
Image-Pro IQbase - Expandable • Suitable for single-user, small workgroup, and enterprise-level deployment • Seats are easily added • Database can handle virtually unlimited entries Confidential - Do not copy or distribute
Image-Pro IQbase - Intelligent • Automatically remembers user settings and preferences • Understands and collects data from within Image-Pro Plus automatically • Reports can be produced in a variety of formats (PDF, HTML, RTF, etc. ) Confidential - Do not copy or distribute
Image-Pro IQbase - Programmable • Includes Visual Basic for Applications® • Create custom forms, tools and plugins from within the product Confidential - Do not copy or distribute
IQbase • Top Features 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. Context Sensitive Searches Easy Addition of files to the database Image-Pro measurements savable and searchable Savable searches Multi-user login with optional passwords Administration Data display Report generator produces a variety of formats Customizable interface features Visual Basic for Applications Offline Storage Confidential - Do not copy or distribute
Who Will Buy IQbase? • New Image-Pro customers (plug-in) – Archive measurements automatically • Existing Image-Pro users – Network installations of IPP • Near-line storage systems • Researchers and organizations that need to improve their efficiency Confidential - Do not copy or distribute
IQbase • Database product that includes programming, reporting, and image analysis features – Built for organizations that want to archive their data and mine it for information at a later time – Perfect for organizations that need to communicate their findings both internally and externally – Ideal for those interested in combining powerful data management with image analysis Confidential - Do not copy or distribute
Future Product Discussions
Business Session
HEADQUARTERS Media Cybernetics, Inc. 8484 Georgia Avenue, Suite 200 Silver Spring, MD 20910 USA Phone: + 1 301 495 3305 Fax: + 1 301 495 5964 Email: info@mediacy. com Web: www. mediacy. com Confidential - Do not copy or distribute
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